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NAMPT/PBEF Polyclonal antibody
NAMPT/PBEF Polyclonal Antibody for IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat, zebrafish
Applications
WB, IP, IHC, IF,ELISA
Conjugate
Unconjugated
Cat no : 11776-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | rat liver tissue, mouse skeletal muscle tissue, zebrafish tissue, HL-60 cells, RAW 264.7 cells, Ramos cells, mouse liver tissue, mouse heart tissue |
| Positive IP detected in | mouse heart tissue |
| Positive IHC detected in | human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 4 publications below |
| WB | See 15 publications below |
| IHC | See 4 publications below |
| IF | See 2 publications below |
Product Information
11776-1-AP targets NAMPT/PBEF in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat, zebrafish samples.
| Tested Reactivity | human, mouse, rat, zebrafish |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | NAMPT/PBEF fusion protein Ag2434 |
| Full Name | nicotinamide phosphoribosyltransferase |
| Calculated molecular weight | 52 kDa |
| Observed molecular weight | 50-55 kDa |
| GenBank accession number | BC020691 |
| Gene symbol | NAMPT |
| Gene ID (NCBI) | 10135 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Nicotinamide phosphoribosyltransferase(NAMPT) has two usual synonyms termed Visfatin and PBEF. Its primary role is to catalyze the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD, which is the rate limiting component in the mammalian NAD biosynthesis pathway. NAMPT is expressed in large amounts in bone marrow, liver tissue, and muscle tissues. NAMPT inhibits neutrophil apoptosis in experimental inflammation and clinical sepsis. NAMPT levels are altered in plasma of patients with type 2 diabetes mellitus (T2DM), and it is now evidenced that NAMPT may plays a role in lipid metabolism.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NAMPT/PBEF antibody 11776-1-AP | Download protocol |
| IHC protocol for NAMPT/PBEF antibody 11776-1-AP | Download protocol |
| IP protocol for NAMPT/PBEF antibody 11776-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Front Oncol NAMPT and NAPRT, Key Enzymes in NAD Salvage Synthesis Pathway, Are of Negative Prognostic Value in Colorectal Cancer. | ||
Antioxidants (Basel) Dietary Antioxidant Trans-Cinnamaldehyde Reduced Visfatin-Induced Breast Cancer Progression: In Vivo and In Vitro Study. | ||
J Mol Med (Berl) Exogenous H2S switches cardiac energy substrate metabolism by regulating SIRT3 expression in db/db mice. | ||
Acta Cir Bras Effect of curcumin on visfatin and zinc-α2-glycoprotein in a rat model of non-alcoholic fatty liver disease. | ||
Hum Exp Toxicol Nicotinamide improves NAD+ levels to protect against acetaminophen-induced acute liver injury in mice. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH kishu (Verified Customer) (06-10-2021) | this Ab works well for WB (1:100) and IHC (1:200)
|
FH Iru (Verified Customer) (05-30-2019) | This antibody works really well.
|
