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  • KD/KO Validated

NAMPT/PBEF Polyclonal antibody

NAMPT/PBEF Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat, zebrafish





Cat no : 11776-1-AP


1110035O14Rik, NAmPRTase, NAMPT, NAMPT/PBEF, PBEF, PBEF1, Pre B cell enhancing factor, VF, VISFATIN

Tested Applications

Positive WB detected inrat liver tissue, Ramos cells, mouse heart tissue, HL-60 cells, RAW 264.7 cells, mouse liver tissue, mouse skeletal muscle tissue
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells, HeLa cells, U-87 MG cells

Recommended dilution

Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11776-1-AP targets NAMPT/PBEF in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat, zebrafish samples.

Tested Reactivity human, mouse, rat, zebrafish
Cited Reactivityhuman, mouse, rat, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NAMPT/PBEF fusion protein Ag2434
Full Name nicotinamide phosphoribosyltransferase
Calculated Molecular Weight 52 kDa
Observed Molecular Weight 50-55 kDa
GenBank Accession NumberBC020691
Gene Symbol NAMPT
Gene ID (NCBI) 10135
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Nicotinamide phosphoribosyltransferase(NAMPT) has two usual synonyms termed Visfatin and PBEF. Its primary role is to catalyze the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD, which is the rate limiting component in the mammalian NAD biosynthesis pathway. NAMPT is expressed in large amounts in bone marrow, liver tissue, and muscle tissues. NAMPT inhibits neutrophil apoptosis in experimental inflammation and clinical sepsis. NAMPT levels are altered in plasma of patients with type 2 diabetes mellitus (T2DM), and it is now evidenced that NAMPT may plays a role in lipid metabolism.


Product Specific Protocols
WB protocol for NAMPT/PBEF antibody 11776-1-APDownload protocol
IHC protocol for NAMPT/PBEF antibody 11776-1-APDownload protocol
IF protocol for NAMPT/PBEF antibody 11776-1-APDownload protocol
IP protocol for NAMPT/PBEF antibody 11776-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Cancer Cell

Inhibition of De Novo NAD(+) Synthesis by Oncogenic URI Causes Liver Tumorigenesis through DNA Damage.

Authors - Krishna S Tummala

Cell Metab

NAD+ Metabolism Maintains Inducible PD-L1 Expression to Drive Tumor Immune Evasion.

Authors - Hongwei Lv
  • KD Validated

Cell Death Dis

Astrocyte-derived exosomal nicotinamide phosphoribosyltransferase (Nampt) ameliorates ischemic stroke injury by targeting AMPK/mTOR signaling to induce autophagy

Authors - Yang Deng

Int J Mol Sci

NAD+ Anabolism Disturbance Causes Glomerular Mesangial Cell Injury in Diabetic Nephropathy.

Authors - Xue Li


Hyperglycemia-reduced NAD+ biosynthesis impairs corneal epithelial wound healing in diabetic mice.

Authors - Ya Li
  • KD Validated


Inhibition of nicotinamide phosphoribosyltransferase (NAMPT) with OT-82 induces DNA damage, cell death, and suppression of tumor growth in preclinical models of Ewing sarcoma.

Authors - Anna E Gibson
  • KD Validated


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


DAN (Verified Customer) (03-11-2022)

Very effective in getting targeted efficacy.

  • Applications: Western Blot
  • Cell Tissue Type: Human, Mouse, Rat

kishu (Verified Customer) (06-10-2021)

this Ab works well for WB (1:100) and IHC (1:200)

  • Applications: Western Blot, Immunohistochemistry
  • Primary Antibody Dilution: 1;1000
  • Cell Tissue Type: mouse liver tissues and rat liver

Iru (Verified Customer) (05-30-2019)

This antibody works really well.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: fallopian tube epithelial cells