NAMPT/PBEF Polyclonal antibody

• 

  WB analysis using 11776-1-AP

  Rat liver lysates were subjected to SDS PAGE followed by western blot with 11776-1-AP (NAMPT/PBEF antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Ramos using 11776-1-AP

  Ramos cells were subjected to SDS PAGE followed by western blot with 11776-1-AP (NAMPT/PBEF antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Ramos using 11776-1-AP

  Ramos cells were subjected to SDS PAGE followed by western blot with 11776-1-AP (NAMPT/PBEF antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 11776-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11776-1-AP (NAMPT/PBEF antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 11776-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11776-1-AP (NAMPT/PBEF antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 11776-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 11776-1-AP (NAMPT/PBEF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 11776-1-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 11776-1-AP (NAMPT/PBEF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 11776-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 11776-1-AP (NAMPT/PBEF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of zebrafish using 11776-1-AP

  zebrafish tissue were subjected to SDS PAGE followed by western blot with 11776-1-AP (NAMPT/PBEF antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse heart using 11776-1-AP

  IP result of anti-NAMPT/PBEF (IP:11776-1-AP, 4ug; Detection:11776-1-AP 1:1000) with mouse heart tissue lysate 3720 ug.

• 

  IP experiment of mouse heart using 11776-1-AP

  IP result of anti-NAMPT/PBEF (IP:11776-1-AP, 4ug; Detection:11776-1-AP 1:4000) with mouse heart tissue lysate 1560 ug.

• 

  IHC staining of human lymphoma using 11776-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11776-1-AP (NAMPT/PBEF antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 11776-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11776-1-AP (NAMPT/PBEF antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HEK-293 using 11776-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using NAMPT/PBEF antibody (11776-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  IF Staining of U-87 MG using 11776-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U-87 MG cells using NAMPT/PBEF antibody (11776-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 11776-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using NAMPT/PBEF antibody (11776-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

NAMPT/PBEF Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 11776-1-AP

Publications(53)

Reviews (4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, zebrafish

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Nampt, Visfatin, Pre B cell enhancing factor, PBEF1, PBEF

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Tested Applications

• Positive WB detected in: rat liver tissue, Ramos cells, mouse heart tissue, HL-60 cells, RAW 264.7 cells, mouse liver tissue, mouse skeletal muscle tissue
• Positive IP detected in: mouse heart tissue
• Positive IHC detected in: human lymphoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HEK-293 cells, HeLa cells, U-87 MG cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 8 publications below
• WB: See 48 publications below
• IHC: See 12 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Product Information

11776-1-AP targets NAMPT/PBEF in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, zebrafish samples.

• Tested Reactivity: human, mouse, rat, zebrafish
• Cited Reactivity: human, mouse, rat, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NAMPT/PBEF fusion protein Ag2434
• Full Name: nicotinamide phosphoribosyltransferase
• Calculated Molecular Weight: 52 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: BC020691
• Gene Symbol: NAMPT/PBEF
• Gene ID (NCBI): 10135
• RRID: AB_2298317
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P43490
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Nicotinamide phosphoribosyltransferase(NAMPT) has two usual synonyms termed Visfatin and PBEF. Its primary role is to catalyze the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD, which is the rate limiting component in the mammalian NAD biosynthesis pathway. NAMPT is expressed in large amounts in bone marrow, liver tissue, and muscle tissues. NAMPT inhibits neutrophil apoptosis in experimental inflammation and clinical sepsis. NAMPT levels are altered in plasma of patients with type 2 diabetes mellitus (T2DM), and it is now evidenced that NAMPT may plays a role in lipid metabolism.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Cancer Cell; Inhibition of De Novo NAD(+) Synthesis by Oncogenic URI Causes Liver Tumorigenesis through DNA Damage.; Authors - Krishna S Tummala
human,mouse	IHC,WB	Cell Metab; NAD+ Metabolism Maintains Inducible PD-L1 Expression to Drive Tumor Immune Evasion.; Authors - Hongwei Lv ; KD Validated
mouse	WB,IHC	Cell Death Dis; Astrocyte-derived exosomal nicotinamide phosphoribosyltransferase (Nampt) ameliorates ischemic stroke injury by targeting AMPK/mTOR signaling to induce autophagy; Authors - Yang Deng
human,mouse	WB,IHC	Int J Mol Sci; NAD+ Anabolism Disturbance Causes Glomerular Mesangial Cell Injury in Diabetic Nephropathy.; Authors - Xue Li
human,mouse	IF,WB	Metabolism; Hyperglycemia-reduced NAD+ biosynthesis impairs corneal epithelial wound healing in diabetic mice.; Authors - Ya Li ; KD Validated
human	WB	Oncogenesis; Inhibition of nicotinamide phosphoribosyltransferase (NAMPT) with OT-82 induces DNA damage, cell death, and suppression of tumor growth in preclinical models of Ewing sarcoma.; Authors - Anna E Gibson ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Lenie (Verified Customer) (07-04-2025)

Included in WB (mitochondrial muscle preparation, dry blotting, used 5% milk in TBST as blocking, jad a lot of background binding) and IF (on a freeze dried sample on muscles)

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1/200 (IF) and 1/1000 (WB)
• Cell Tissue Type: Zebrafish

NAMPT/PBEF Antibody Western Blot,Immunofluorescence validation (1/200 (IF) and 1/1000 (WB) dilution) in Zebrafish (Cat no:11776-1-AP)

FH

DAN (Verified Customer) (03-11-2022)

Very effective in getting targeted efficacy.

• Applications: Western Blot
• Cell Tissue Type: Human, Mouse, Rat

FH

kishu (Verified Customer) (06-10-2021)

this Ab works well for WB (1:100) and IHC (1:200)

• Applications: Western Blot, Immunohistochemistry
• Primary Antibody Dilution: 1;1000
• Cell Tissue Type: mouse liver tissues and rat liver

FH

Iru (Verified Customer) (05-30-2019)

This antibody works really well.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: fallopian tube epithelial cells