NAMPT/PBEF Polyclonal antibody

• Featured Product
• KD/KO Validated

NAMPT/PBEF Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, zebrafish

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

20

Reviews (3)

Cat no : 11776-1-AP

Synonyms

1110035O14Rik, NAmPRTase, NAMPT, PBEF, PBEF1, Pre B cell enhancing factor, VF, VISFATIN

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Validation Data Gallery

• 

  WB analysis using 11776-1-AP

  Rat liver lysates were subjected to SDS PAGE followed by western blot with 11776-1-AP (NAMPT/PBEF antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 11776-1-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 11776-1-AP (NAMPT/PBEF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 11776-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 11776-1-AP (NAMPT/PBEF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 11776-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 11776-1-AP (NAMPT/PBEF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Ramos using 11776-1-AP

  Ramos cells were subjected to SDS PAGE followed by western blot with 11776-1-AP (NAMPT/PBEF antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 11776-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11776-1-AP (NAMPT/PBEF antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of zebrafish using 11776-1-AP

  zebrafish tissue were subjected to SDS PAGE followed by western blot with 11776-1-AP (NAMPT/PBEF antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 11776-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11776-1-AP (NAMPT/PBEF antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse heart using 11776-1-AP

  IP result of anti-NAMPT/PBEF (IP:11776-1-AP, 4ug; Detection:11776-1-AP 1:1000) with mouse heart tissue lysate 3720 ug.

• 

  IHC staining of human lymphoma using 11776-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11776-1-AP (NAMPT/PBEF antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 11776-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11776-1-AP (NAMPT/PBEF antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: rat liver tissue, mouse skeletal muscle tissue, zebrafish tissue, HL-60 cells, RAW 264.7 cells, Ramos cells, mouse liver tissue, mouse heart tissue
• Positive IP detected in: mouse heart tissue
• Positive IHC detected in: human lymphoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
• Immunohistochemistry (IHC): IHC : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 4 publications below
• WB: See 18 publications below
• IHC: See 5 publications below
• IF: See 2 publications below

Product Information

11776-1-AP targets NAMPT/PBEF in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat, zebrafish samples.

• Tested Reactivity: human, mouse, rat, zebrafish
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NAMPT/PBEF fusion protein Ag2434
• Full Name: nicotinamide phosphoribosyltransferase
• Calculated molecular weight: 52 kDa
• Observed molecular weight: 50-55 kDa
• GenBank accession number: BC020691
• Gene symbol: NAMPT
• Gene ID (NCBI): 10135
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Nicotinamide phosphoribosyltransferase(NAMPT) has two usual synonyms termed Visfatin and PBEF. Its primary role is to catalyze the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD, which is the rate limiting component in the mammalian NAD biosynthesis pathway. NAMPT is expressed in large amounts in bone marrow, liver tissue, and muscle tissues. NAMPT inhibits neutrophil apoptosis in experimental inflammation and clinical sepsis. NAMPT levels are altered in plasma of patients with type 2 diabetes mellitus (T2DM), and it is now evidenced that NAMPT may plays a role in lipid metabolism.

Publications

Species	Application	Title
mouse	WB	PLoS One; NAMPT overexpression alleviates alcohol-induced hepatic steatosis in mice.; Authors - Xiwen Xiong
human	WB,IHC	Front Oncol; NAMPT and NAPRT, Key Enzymes in NAD Salvage Synthesis Pathway, Are of Negative Prognostic Value in Colorectal Cancer.; Authors - Xiao-Qin Li
human	WB	Antioxidants (Basel); Dietary Antioxidant Trans-Cinnamaldehyde Reduced Visfatin-Induced Breast Cancer Progression: In Vivo and In Vitro Study.; Authors - Yi-Fen Chiang
mouse	WB	J Mol Med (Berl); Exogenous H2S switches cardiac energy substrate metabolism by regulating SIRT3 expression in db/db mice.; Authors - Yu Sun
human,mouse	WB,IHC	Int J Mol Sci; NAD+ Anabolism Disturbance Causes Glomerular Mesangial Cell Injury in Diabetic Nephropathy.; Authors - Xue Li
rat	WB	Acta Cir Bras; Effect of curcumin on visfatin and zinc-α2-glycoprotein in a rat model of non-alcoholic fatty liver disease.; Authors - Changping Li

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

DAN (Verified Customer) (03-11-2022)

Very effective in getting targeted efficacy.

• Applications: Western Blot
• Cell Tissue Type: Human, Mouse, Rat

FH

kishu (Verified Customer) (06-10-2021)

this Ab works well for WB (1:100) and IHC (1:200)

• Applications: Western Blot, Immunohistochemistry
• Primary Antibody Dilution: 1;1000
• Cell Tissue Type: mouse liver tissues and rat liver

FH

Iru (Verified Customer) (05-30-2019)

This antibody works really well.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: fallopian tube epithelial cells

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