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NAMPT/PBEF Polyclonal antibody

NAMPT/PBEF Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, zebrafish

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11776-1-AP

Synonyms

1110035O14Rik, NAmPRTase, NAMPT, PBEF, PBEF1, Pre B cell enhancing factor, VF, VISFATIN



Tested Applications

Positive WB detected inrat liver tissue, mouse skeletal muscle tissue, zebrafish tissue, HL-60 cells, RAW 264.7 cells, Ramos cells, mouse liver tissue, mouse heart tissue
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

11776-1-AP targets NAMPT/PBEF in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat, zebrafish samples.

Tested Reactivity human, mouse, rat, zebrafish
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NAMPT/PBEF fusion protein Ag2434
Full Name nicotinamide phosphoribosyltransferase
Calculated molecular weight 52 kDa
Observed molecular weight 50-55 kDa
GenBank accession numberBC020691
Gene symbol NAMPT
Gene ID (NCBI) 10135
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Nicotinamide phosphoribosyltransferase(NAMPT) has two usual synonyms termed Visfatin and PBEF. Its primary role is to catalyze the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD, which is the rate limiting component in the mammalian NAD biosynthesis pathway. NAMPT is expressed in large amounts in bone marrow, liver tissue, and muscle tissues. NAMPT inhibits neutrophil apoptosis in experimental inflammation and clinical sepsis. NAMPT levels are altered in plasma of patients with type 2 diabetes mellitus (T2DM), and it is now evidenced that NAMPT may plays a role in lipid metabolism.

Protocols

Product Specific Protocols
WB protocol for NAMPT/PBEF antibody 11776-1-APDownload protocol
IHC protocol for NAMPT/PBEF antibody 11776-1-APDownload protocol
IP protocol for NAMPT/PBEF antibody 11776-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

PLoS One

NAMPT overexpression alleviates alcohol-induced hepatic steatosis in mice.

Authors - Xiwen Xiong
humanWB,IHC

Front Oncol

NAMPT and NAPRT, Key Enzymes in NAD Salvage Synthesis Pathway, Are of Negative Prognostic Value in Colorectal Cancer.

Authors - Xiao-Qin Li
humanWB

Antioxidants (Basel)

Dietary Antioxidant Trans-Cinnamaldehyde Reduced Visfatin-Induced Breast Cancer Progression: In Vivo and In Vitro Study.

Authors - Yi-Fen Chiang
mouseWB

J Mol Med (Berl)

Exogenous H2S switches cardiac energy substrate metabolism by regulating SIRT3 expression in db/db mice.

Authors - Yu Sun
ratWB

Acta Cir Bras

Effect of curcumin on visfatin and zinc-α2-glycoprotein in a rat model of non-alcoholic fatty liver disease.

Authors - Changping Li
mouseWB

Hum Exp Toxicol

Nicotinamide improves NAD+ levels to protect against acetaminophen-induced acute liver injury in mice.

Authors - J Xu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

kishu (Verified Customer) (06-10-2021)

this Ab works well for WB (1:100) and IHC (1:200)

  • Applications: Western Blot,Immunohistochemistry
  • Primary Antibody Dilution: 1;1000
  • Cell Tissue Type: mouse liver tissues and rat liver
FH

Iru (Verified Customer) (05-30-2019)

This antibody works really well.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: fallopian tube epithelial cells