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NAMPT/PBEF Polyclonal antibody
NAMPT/PBEF Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Cat no : 11776-1-AP
Validation Data Gallery
|Positive WB detected in||rat liver tissue, Ramos cells, mouse heart tissue, HL-60 cells, RAW 264.7 cells, mouse liver tissue, mouse skeletal muscle tissue|
|Positive IP detected in||mouse heart tissue|
|Positive IHC detected in||human lymphoma tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HEK-293 cells, HeLa cells, U-87 MG cells|
|Western Blot (WB)||WB : 1:2000-1:10000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:1000-1:8000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:200-1:800|
|Sample-dependent, check data in validation data gallery|
11776-1-AP targets NAMPT/PBEF in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat, zebrafish samples.
|Tested Reactivity||human, mouse, rat, zebrafish|
|Cited Reactivity||human, mouse, rat, zebrafish|
|Host / Isotype||Rabbit / IgG|
|Immunogen||NAMPT/PBEF fusion protein Ag2434|
|Full Name||nicotinamide phosphoribosyltransferase|
|Calculated molecular weight||52 kDa|
|Observed molecular weight||50-55 kDa|
|GenBank accession number||BC020691|
|Gene ID (NCBI)||10135|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Nicotinamide phosphoribosyltransferase(NAMPT) has two usual synonyms termed Visfatin and PBEF. Its primary role is to catalyze the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD, which is the rate limiting component in the mammalian NAD biosynthesis pathway. NAMPT is expressed in large amounts in bone marrow, liver tissue, and muscle tissues. NAMPT inhibits neutrophil apoptosis in experimental inflammation and clinical sepsis. NAMPT levels are altered in plasma of patients with type 2 diabetes mellitus (T2DM), and it is now evidenced that NAMPT may plays a role in lipid metabolism.
|Product Specific Protocols|
|WB protocol for NAMPT/PBEF antibody 11776-1-AP||Download protocol|
|IHC protocol for NAMPT/PBEF antibody 11776-1-AP||Download protocol|
|IF protocol for NAMPT/PBEF antibody 11776-1-AP||Download protocol|
|IP protocol for NAMPT/PBEF antibody 11776-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Inhibition of De Novo NAD(+) Synthesis by Oncogenic URI Causes Liver Tumorigenesis through DNA Damage.
NAD+ Metabolism Maintains Inducible PD-L1 Expression to Drive Tumor Immune Evasion.
Cell Death Dis
Astrocyte-derived exosomal nicotinamide phosphoribosyltransferase (Nampt) ameliorates ischemic stroke injury by targeting AMPK/mTOR signaling to induce autophagy
Int J Mol Sci
NAD+ Anabolism Disturbance Causes Glomerular Mesangial Cell Injury in Diabetic Nephropathy.
Hyperglycemia-reduced NAD+ biosynthesis impairs corneal epithelial wound healing in diabetic mice.
Inhibition of nicotinamide phosphoribosyltransferase (NAMPT) with OT-82 induces DNA damage, cell death, and suppression of tumor growth in preclinical models of Ewing sarcoma.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
DAN (Verified Customer) (03-11-2022)
Very effective in getting targeted efficacy.
kishu (Verified Customer) (06-10-2021)
this Ab works well for WB (1:100) and IHC (1:200)
Iru (Verified Customer) (05-30-2019)
This antibody works really well.