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NAMPT/PBEF Monoclonal antibody

NAMPT/PBEF Monoclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

CloneNo.

3D4D8

Cat no : 66385-1-Ig

Synonyms

1110035O14Rik, NAmPRTase, NAMPT, PBEF, PBEF1, Pre B cell enhancing factor, VF, VISFATIN



Tested Applications

Positive WB detected inLNCaP cells, human heart tissue, human skeletal muscle tissue, rat heart tissue, mouse skeletal muscle tissue, Neuro-2a cells, HeLa cells, HepG2 cells, K-562 cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells, Jurkat cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

66385-1-Ig targets NAMPT/PBEF in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen NAMPT/PBEF fusion protein Ag2434
Full Name nicotinamide phosphoribosyltransferase
Calculated molecular weight 52 kDa
Observed molecular weight 52 kDa
GenBank accession numberBC020691
Gene symbol NAMPT
Gene ID (NCBI) 10135
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Nicotinamide phosphoribosyltransferase(NAMPT) has two usual synonyms termed Visfatin and PBEF. Its primary role is to catalyze the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD, which is the rate limiting component in the mammalian NAD biosynthesis pathway. NAMPT is localized in cytoplasm and expressed in large amounts in bone marrow, liver tissue, and muscle tissues. NAMPT inhibits neutrophil apoptosis in experimental inflammation and clinical sepsis. NAMPT levels are altered in plasma of patients with type 2 diabetes mellitus (T2DM), and it is now evidenced that NAMPT may plays a role in lipid metabolism.

Protocols

Product Specific Protocols
WB protocol for NAMPT/PBEF antibody 66385-1-IgDownload protocol
IHC protocol for NAMPT/PBEF antibody 66385-1-IgDownload protocol
IF protocol for NAMPT/PBEF antibody 66385-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

J Cell Physiol

Nicotinamide phosphoribosyl transferase regulates cell growth via the Sirt1/P53 signaling pathway and is a prognosis marker in colorectal cancer.

Authors - Jing-Hua Pan
  • KD Validated