NANOG Antibody 13 Publications

Rabbit Polyclonal| Catalog number: 14295-1-AP

Featured Product KD/KO validated

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Freight/Packing

Con: 42 μg/150 μl

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Species specificity:
human, mouse, rat

Positive WB detected in:
rat brain tissue, human brain tissue, mouse brain tissue

Positive IHC detected in:
human lung cancer tissue

Positive IF detected in:
human embronic stem cells

Recommended dilution:
WB : 1:1000-1:4000
IHC : 1:50-1:500
IF : 1:20-1:200

Product Information


Source:
Rabbit

Purification method:
Antigen affinity purification

Isotype:
IgG

Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
Nanog homeobox

Calculated molecular weight:
35 kDa

Observed molecular weight:
43-48 kDa

GenBank accession number:

Gene ID (NCBI):

Gene symbol
NANOG

Synonyms
hNanog, Homeobox protein NANOG, NANOG, Nanog homeobox
Background

Nanog is a member of the homeobox family of DNA binding transcription factors and has been shown to maintain embryonic stem (ES) cell self-renewal independently of leukemia inhibitory factor (LIF)/Stat3. Nanog mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Functionally, Nanog works together with other key pluripotent factors (Oct4, Sox2, and Lin28) to reprogram human fibroblasts and generate induced pluripotent stem (iPS) cells. These key factors form a regulatory network to support or limit each other's expression level, which maintains the properties of ES cells. Affinity purified rabbit anti-Nanog can be used to demonstrate pluripotency of ES and IPS cells. There are two kinds of variants could recognized by NANOG, one is normal form (~39kd), the other is PTD form (~48kd) (21136380 )


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