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NANOG Polyclonal antibody

NANOG Polyclonal Antibody for WB, IF, FC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (2)





Cat no : 14295-1-AP


hNanog, Homeobox protein NANOG, NANOG, Nanog homeobox

Tested Applications

Positive WB detected inNCCIT cells, rat brain tissue, mouse brain tissue, mouse embryo tissue
Positive IF detected inhuman embronic stem cells
Positive FC detected inNICCT

Recommended dilution

Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)IF : 1:20-1:200
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14295-1-AP targets NANOG in WB, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig, marmoset
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NANOG fusion protein Ag5645
Full Name Nanog homeobox
Calculated Molecular Weight 35 kDa
Observed Molecular Weight35-40 kDa
GenBank Accession NumberBC160187
Gene Symbol NANOG
Gene ID (NCBI) 79923
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Nanog is a member of the homeobox family of DNA binding transcription factors and has been shown to maintain embryonic stem (ES) cell self-renewal independently of leukemia inhibitory factor (LIF)/Stat3. Nanog mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Functionally, Nanog works together with other key pluripotent factors (Oct4, Sox2, and Lin28) to reprogram human fibroblasts and generate induced pluripotent stem (iPS) cells. These key factors form a regulatory network to support or limit each other's expression level, which maintains the properties of ES cells. Affinity purified rabbit anti-Nanog can be used to demonstrate pluripotency of ES and IPS cells. There are two kinds of variants could recognized by NANOG, one is normal form (~39kd), the other is post-translation modified form (~48kd) (21136380 ). Nanog exists two isoforms with molecular weight 34.4 kDa and 31.9 kDa. (PMID: 21969378)


Product Specific Protocols
WB protocol for NANOG antibody 14295-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Fatemeh (Verified Customer) (05-22-2023)

50 ug of protein lysate was used for WB. Blot was blocked with 5% milk and incubated with 1-1000 primary antibody overnight at 4C.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1-1000
  • Cell Tissue Type: mESCs
NANOG Antibody Western Blot validation (1-1000 dilution) in mESCs (Cat no:14295-1-AP)

Udesh (Verified Customer) (02-24-2023)

Worked well at 1:1000 in 5% BSA O/N at 4 degrees

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: U2OS Osteosarcoma cells
NANOG Antibody Western Blot validation (1:1000 dilution) in U2OS Osteosarcoma cells (Cat no:14295-1-AP)

Ying (Verified Customer) (08-19-2019)

very good antibody.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1000
  • Cell Tissue Type: mouse primary cardiomyocytes