|Positive WB detected in||NCCIT cells, rat brain tissue, mouse brain tissue, mouse embryo tissue|
|Positive IF detected in||human embronic stem cells|
|Positive FC detected in||NCCIT cells|
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
14295-1-AP targets NANOG in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, pig, marmoset|
|Host / Isotype||Rabbit / IgG|
|Immunogen||NANOG fusion protein Ag5645|
|Full Name||Nanog homeobox|
|Calculated molecular weight||35 kDa|
|Observed molecular weight||35-40 kDa|
|GenBank accession number||BC160187|
|Gene ID (NCBI)||79923|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Nanog is a member of the homeobox family of DNA binding transcription factors and has been shown to maintain embryonic stem (ES) cell self-renewal independently of leukemia inhibitory factor (LIF)/Stat3. Nanog mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Functionally, Nanog works together with other key pluripotent factors (Oct4, Sox2, and Lin28) to reprogram human fibroblasts and generate induced pluripotent stem (iPS) cells. These key factors form a regulatory network to support or limit each other's expression level, which maintains the properties of ES cells. Affinity purified rabbit anti-Nanog can be used to demonstrate pluripotency of ES and IPS cells. There are two kinds of variants could recognized by NANOG, one is normal form (~39kd), the other is post-translation modified form (~48kd) (21136380 ). Nanog exists two isoforms with molecular weight 34.4 kDa and 31.9 kDa. (PMID: 21969378)
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Fatemeh (Verified Customer) (05-22-2023)
50 ug of protein lysate was used for WB. Blot was blocked with 5% milk and incubated with 1-1000 primary antibody overnight at 4C.
Udesh (Verified Customer) (02-24-2023)
Worked well at 1:1000 in 5% BSA O/N at 4 degrees
Ying (Verified Customer) (08-19-2019)
very good antibody.