NBCn1 Polyclonal antibody

• 

  WB analysis using 29442-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29442-1-AP (NBCn1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

NBCn1 Polyclonal Antibody for WB, ELISA

Cat No. 29442-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

WB, ELISA

Synonyms

Bicarbonate transporter, BT, DKFZp686H168, NBC2, NBC2B, NBC3, NBCn1, SBC2, SLC4A6, SLC4A7

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Tested Applications

• Positive WB detected in: BGC-823 cells, HEK-293 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

29442-1-AP targets NBCn1 in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NBCn1 fusion protein Ag30545
• Full Name: solute carrier family 4, sodium bicarbonate cotransporter, member 7
• Calculated Molecular Weight: 136 kDa
• Observed Molecular Weight: 136 kDa
• GenBank Accession Number: NM_003615
• Gene Symbol: NBCn1/SLC4A7
• Gene ID (NCBI): 9497
• RRID: AB_3086133
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9Y6M7
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SLC4A7 also known as NBCn1, NBC3, NBC2, is an electroneutral Na(+)/HCO(3)(-) cotransporter (PMID: 14736710). SLC4A7 mediates the coupled movement of sodium and bicarbonate ions across the plasma membrane. SLC4A7 plays a key role in macrophage acidification, mediating bicarbonate import into the cytoplasm which is crucial for net acid extrusion and maintenance of cytoplasmic pH during phagocytosis (PubMed:29779931). SLC4A7 is expressed in testis, spleen, and retina. SLC4A7 may be a major regulator of extracellular pH of retina where light stimulation produces an extracellular alkalization and may contribute as a solute transporter to the prevention of retinal detachment(PMID: 9610397).

Publications

Species	Application	Title
human	WB	Cell Rep; Carbonic anhydrase 2 facilitates sorafenib resistance by counteracting MCT4-mediated intracellular pH dysregulation in HCC; Authors - Hui Lu