|Positive WB detected in||human brain tissue, HepG2 cells|
|Positive IP detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Sample-dependent, check data in validation data gallery|
17262-1-AP targets NDEL1 in WB, IP, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||NDEL1 fusion protein Ag10975|
|Full Name||nudE nuclear distribution gene E homolog (A. nidulans)-like 1|
|Calculated molecular weight||345 aa, 38 kDa, 41 kDa|
|Observed molecular weight||38-42 kDa|
|GenBank accession number||BC026101|
|Gene ID (NCBI)||81565|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Nuclear distribution factor E-homolog 1 (NDE1), NDE-like 1 (NDEL1), and Lissencephaly 1 (LIS1) together involve in essential neurodevelopmental processes, including neuronal precursor proliferation and differentiation, neurite outgrowth, and neuronal migration. All three bind directly to Disrupted in Schizophrenia 1 (DISC1), a scaffold protein critical to neuronal proliferation, integration, migration, and synaptic function within the developing and adult brain.[PMID: 18469341] Nuclear distribution element-like 1 (NDEL1) was firstly identified as a regulator of the cytoskeleton in microtubule and intermediate fliament dynamics and microtubule-based transport [PMID:21948775]. It is required for organization of the cellular microtubule array and microtubule anchoring at the centrosome, which is in part by targeting the microtubule severing protein KATNA1 to the centrosome[PMID:15728732].
Extraciliary roles of the ciliopathy protein JBTS17 in mitosis and neurogenesis.
Regulation of the actin cytoskeleton by the Ndel1-Tara complex is critical for cell migration.
Sequential phosphorylation of NDEL1 by the DYRK2-GSK3β complex is critical for neuronal morphogenesis.
J Cell Biol
Lis1 is essential for cortical microtubule organization and desmosome stability in the epidermis.