NDEL1 Polyclonal antibody

NDEL1 Polyclonal Antibody for IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF,ELISA

Conjugate

Unconjugated

Cat no : 17262-1-AP

Synonyms

EOPA, MITAP1, Mitosin associated protein 1, NDEL1, NUDEL, Protein Nudel



Tested Applications

Positive WB detected inhuman brain tissue, HepG2 cells
Positive IP detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Sample-dependent, check data in validation data gallery

Product Information

17262-1-AP targets NDEL1 in WB, IP, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NDEL1 fusion protein Ag10975
Full Name nudE nuclear distribution gene E homolog (A. nidulans)-like 1
Calculated molecular weight 345 aa, 38 kDa, 41 kDa
Observed molecular weight 38-42 kDa
GenBank accession numberBC026101
Gene symbol NDEL1
Gene ID (NCBI) 81565
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Nuclear distribution factor E-homolog 1 (NDE1), NDE-like 1 (NDEL1), and Lissencephaly 1 (LIS1) together involve in essential neurodevelopmental processes, including neuronal precursor proliferation and differentiation, neurite outgrowth, and neuronal migration. All three bind directly to Disrupted in Schizophrenia 1 (DISC1), a scaffold protein critical to neuronal proliferation, integration, migration, and synaptic function within the developing and adult brain.[PMID: 18469341] Nuclear distribution element-like 1 (NDEL1) was firstly identified as a regulator of the cytoskeleton in microtubule and intermediate fliament dynamics and microtubule-based transport [PMID:21948775]. It is required for organization of the cellular microtubule array and microtubule anchoring at the centrosome, which is in part by targeting the microtubule severing protein KATNA1 to the centrosome[PMID:15728732].

Protocols

Product Specific Protocols
WB protocol for NDEL1 antibody 17262-1-APDownload protocol
IP protocol for NDEL1 antibody 17262-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Ann Neurol

Extraciliary roles of the ciliopathy protein JBTS17 in mitosis and neurogenesis.

Authors - Hyowon Hong
humanWB,IF

Sci Rep

Regulation of the actin cytoskeleton by the Ndel1-Tara complex is critical for cell migration.

Authors - Ji-Ho Hong
mouseIP, WB

Elife

Sequential phosphorylation of NDEL1 by the DYRK2-GSK3β complex is critical for neuronal morphogenesis.

Authors - Youngsik Woo
mouseWB,IF

J Cell Biol

Lis1 is essential for cortical microtubule organization and desmosome stability in the epidermis.

Authors - Sumigray Kaelyn D KD