NDFIP2 Polyclonal antibody

• 

  WB analysis using 32043-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32043-1-AP (NDFIP2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

NDFIP2 Polyclonal Antibody for WB, ELISA

Cat No. 32043-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Putative MAPK-activating protein PM04/PM05/PM06/PM07, NEDD4 WW domain-binding protein 5A, NEDD4 family-interacting protein 2, Nedd4 Family Interacting Protein 2, N4WBP5A

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Tested Applications

• Positive WB detected in: A431 cells, K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

32043-1-AP targets NDFIP2 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NDFIP2 fusion protein Ag36480
• Full Name: Nedd4 family interacting protein 2
• Calculated Molecular Weight: 36kDa，336aa
• Observed Molecular Weight: 39 kDa
• GenBank Accession Number: NM_019080.2
• Gene Symbol: NDFIP2
• Gene ID (NCBI): 54602
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q9NV92
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NDFIP2 (Nedd4 family interacting protein 2) is a gene encoding a membrane-anchored adapter protein involved in a variety of cellular processes. It regulates protein ubiquitination and degradation by binding to the Nedd4 family of E3 ubiquitin ligases.NDFIP2 has a wide intracellular distribution, including the Golgi, mitochondria, and perinuclear region of the cytoplasm. Its function involves negative regulation of gene expression, negative regulation of protein transport, and positive regulation of protein ubiquitination. In addition, NDFIP2 is associated with a variety of diseases, such as supratentorial ventricular meningiomas in children. In the cardiovascular field, NDFIP2 affects cardiac electrophysiologic function by regulating the degradation of hERG potassium channels. It is also involved in the immune response, affecting T cell function by regulating cytokine signaling pathways. In oncology, NDFIP2 has been found to affect the antiviral response by targeting IFITM3.