NDNL2 Polyclonal antibody

NDNL2 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG


Human and More (1)





Cat no : 27488-1-AP


HCA4, MAGE G1 antigen, MAGEG1, MAGEL3, Melanoma associated antigen G1, NDNL2, necdin like 2, Necdin like protein 2, NSE3, NSMCE3

Tested Applications

Positive WB detected inHepG2 cells, L02 cells, HeLa cells, human placenta tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

27488-1-AP targets NDNL2 in WB, IHC, IF, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NDNL2 fusion protein Ag26663
Full Name necdin-like 2
Calculated Molecular Weight 34 kDa
Observed Molecular Weight 38 kDa
GenBank Accession NumberBC053999
Gene Symbol NDNL2
Gene ID (NCBI) 56160
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NDNL2 (also known as NSMCE3 or MAGEG1) is a part of the SMC5-6 protein complex that is essential for DNA damage response and chromosome segregation (PMID: 18086888; 27427983). The gene of human NDNL2 maps to chromosome 15q13.1. Northern blot analysis detected expression of a 1.9-kb transcript in all human tissues tested, with highest expression in testis (PMID: 11782285). Missense mutations in NSMCE3 have been associated with an autosomal recessive chromosome breakage syndrome that leads to defective T and B cell function and acute respiratory distress syndrome in early childhood (PMID: 27427983).


Product Specific Protocols
WB protocol for NDNL2 antibody 27488-1-APDownload protocol
IHC protocol for NDNL2 antibody 27488-1-APDownload protocol
IF protocol for NDNL2 antibody 27488-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Cell Rep

Epstein-Barr virus BNRF1 destabilizes SMC5/6 cohesin complexes to evade its restriction of replication compartments.

Authors - Stephanie Pei Tung Yiu