NDUFS3 Polyclonal antibody

NDUFS3 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 15066-1-AP

Synonyms

CI 30kD, Complex I 30kD, NDUFS3



Tested Applications

Positive WB detected inHEK-293T cells, HepG2 cells, mouse heart tissue
Positive IP detected inmouse heart tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

15066-1-AP targets NDUFS3 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, pig, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NDUFS3 fusion protein Ag7072
Full Name NADH dehydrogenase (ubiquinone) Fe-S protein 3, 30kDa (NADH-coenzyme Q reductase)
Calculated molecular weight 30 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC000617
Gene symbol NDUFS3
Gene ID (NCBI) 4722
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NDUFS3(NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial) is also named as CI-30kD and belongs to the complex I 30 kDa subunit family. It catalyzes the oxidation of NADH, with the concomitant reduction of ubiquinone and ejection of protons out of the mitochondria(PMID:10967146). NDUFS3 can be used as a mitochondrial matrix control(PMID:17344420). The full length has a transit peptide with 36 amino acids.

Protocols

Product Specific Protocols
WB protocol for NDUFS3 antibody 15066-1-APDownload protocol
IHC protocol for NDUFS3 antibody 15066-1-APDownload protocol
IF protocol for NDUFS3 antibody 15066-1-APDownload protocol
IP protocol for NDUFS3 antibody 15066-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Biol Chem

The cellular stress proteins CHCHD10 and MNRR1 (CHCHD2): Partners in mitochondrial and nuclear function and dysfunction.

Authors - Neeraja Purandare
humanWB

JCI Insight

Extensive skeletal muscle cell mitochondriopathy distinguishes critical limb ischemia patients from claudicants.

Authors - Terence E Ryan
ratIHC

Exp Biol Med (Maywood)

Gene expression profiling in glomeruli of diabetic nephropathy rat.

Authors - Zhang Qian Q
humanWB

Life Sci

SR18292 exerts potent antitumor effects in multiple myeloma via inhibition of oxidative phosphorylation.

Authors - Yu Xiang
humanWB

Invest Ophthalmol Vis Sci

Leber's Hereditary Optic Neuropathy Arising From the Synergy Between ND1 3635G>A Mutation and Mitochondrial YARS2 Mutations.

Authors - Xiaofen Jin
mouseWB

FEBS Lett

Apoptosis-inducing factor is a target gene of C/EBPα and participates in adipocyte differentiation.

Authors - Shen Shao-Ming SM