- Featured Product
- KD/KO Validated
NDUFS3 Polyclonal antibody
NDUFS3 Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Cat no : 15066-1-AP
|Positive WB detected in
|HEK-293T cells, HepG2 cells, mouse brain tissue, mouse heart tissue, rat heart tissue, rat brain tissue, NIH/3T3 cells
|Positive IP detected in
|mouse heart tissue
|Positive IHC detected in
|mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|Western Blot (WB)
|WB : 1:2000-1:10000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:50-1:500
|IF : 1:50-1:500
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
15066-1-AP targets NDUFS3 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|human, mouse, rat
|human, mouse, rat, pig
|Host / Isotype
|Rabbit / IgG
|NDUFS3 fusion protein Ag7072
|NADH dehydrogenase (ubiquinone) Fe-S protein 3, 30kDa (NADH-coenzyme Q reductase)
|Calculated molecular weight
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
NDUFS3(NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial) is also named as CI-30kD and belongs to the complex I 30 kDa subunit family. It catalyzes the oxidation of NADH, with the concomitant reduction of ubiquinone and ejection of protons out of the mitochondria(PMID:10967146). NDUFS3 can be used as a mitochondrial matrix control(PMID:17344420). The full length has a transit peptide with 36 amino acids.
|Product Specific Protocols
|WB protocol for NDUFS3 antibody 15066-1-AP
|IHC protocol for NDUFS3 antibody 15066-1-AP
|IF protocol for NDUFS3 antibody 15066-1-AP
|IP protocol for NDUFS3 antibody 15066-1-AP
|Click here to view our Standard Protocols
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