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NF-M Antibody 1 Publications

Rabbit Polyclonal| Catalog number: 25805-1-AP

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Con: 32 μg/150 μl

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Tested Applications

Applications:
WB, IHC, IF, ELISA
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Images See all

NF-M Antibody IHC mouse cerebellum tissue 25805-1-AP

Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 25805-1-AP (NF-M antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

NF-M Antibody IF mouse brain tissue 25805-1-AP

Immunofluorescent analysis of ( 4% PFA) fixed mouse brain tissue using 25805-1-AP (NF-M antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

NF-M Antibody IHC mouse cerebellum tissue 25805-1-AP

Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 25805-1-AP (NF-M antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

NF-M Antibody WB mouse brain tissue 25805-1-AP

mouse brain tissue were subjected to SDS PAGE followed by western blot with 25805-1-AP( NEFM Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

Species specificity:
human, rat, mouse
Positive WB detected in:
mouse brain tissue, rat brain tissue
Positive IHC detected in:
mouse cerebellum tissue, human brain tissue, human colon tissue, mouse brain tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
mouse brain tissue
Recommended dilution:
WB : 1:500-1:2000
IHC : 1:50-1:500
IF : 1:50-1:500
Sample-dependent, check data in Validation tab

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
NF-M fusion protein Ag22709
Full name:
neurofilament, medium polypeptide
Calculated molecular weight:
102 kDa
Observed molecular weight:
140 kDa
GenBank accession number:
BC002421
Gene ID (NCBI):
4741
Gene symbol
NEFM
Synonyms
160 kDa neurofilament protein, NEF3, NEFM, Neurofilament 3, NF M, NFM

Background

NEFM, also named as NEF3 and NFM, belongs to the intermediate filament family. Neurofilaments are the 10nm intermediate filaments found specifically in neurons. They are a major component of the cell's cytoskeleton, and provide support for normal axonal radial growth. Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. The names given to the three major neurofilament subunits are based upon the apparent molecular weight of the mammalian subunits on SDS-PAGE:NF-L, 65-68kd; NF-M,145-160kd and NF-H, 200-220kd. This antibody recognizes endogenous NF-M protein.


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