NEUROD1 Polyclonal antibody

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  WB analysis using 12081-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12081-1-AP (NEUROD1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of Y79 using 12081-1-AP

  IP result of anti-NEUROD1 (IP:12081-1-AP, 3ug; Detection:12081-1-AP 1:200) with Y79 cells lysate 2000ug.

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  IHC staining of rat brain using 12081-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 12081-1-AP (NEUROD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 12081-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12081-1-AP (NEUROD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 12081-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12081-1-AP (NEUROD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 12081-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 12081-1-AP (NEUROD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 12081-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12081-1-AP (NEUROD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 12081-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12081-1-AP (NEUROD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 12081-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 12081-1-AP (NEUROD1 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse brain using 12081-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 12081-1-AP (NEUROD1 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

NEUROD1 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

Cat No. 12081-1-AP

Publications(17)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, IP, ELISA

Synonyms

NEUROD, Class A basic helix-loop-helix protein 3, bHLHa3

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Tested Applications

• Positive WB detected in: Y79 cells, mouse pancreas tissue, rat pancreas tissue
• Positive IP detected in: Y79 cells
• Positive IHC detected in: rat brain tissue, human pancreas cancer tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 9 publications below
• IHC: See 6 publications below
• IF: See 3 publications below

Product Information

12081-1-AP targets NEUROD1 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NEUROD1 fusion protein Ag2713
• Full Name: neurogenic differentiation 1
• Calculated Molecular Weight: 356 aa, 40 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC009046
• Gene Symbol: NEUROD1
• Gene ID (NCBI): 4760
• RRID: AB_2877823
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q13562
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NeuroD is a member of the basic helix-loop-helix (bHLH) family of transcription factors. The basic helix-loop-helix (bHLH) proteins are transcription factors that are required for several aspects of development, including cell type determination, terminal differentiation and sex determination. Members of the myogenic determination family, MyoD, myf5, myogenin and MRF4, all have bHLH domains.These proteins function by forming heterodimers with E-proteins and binding to the canonical E-box sequence CANNTG. Neuro D is expressed transiently in a subset of neurons in the central and peripheral nervous systems at the time of their terminal differentiation into mature neurons. Moreover, ectopic expression of Neuro D in Xenopus embryos induces premature differentiation of neuronal precursors and Neuro D can convert presumptive epidermal cells into neurons.The lack of NeuroD in the brain results in severe defects in development. Human mutations have been linked to a number of types of diabetes including type I diabetes mellitus and maturity-onset diabetes of the young. The calculated molecular weight of NEUROD1 is 39 kDa, but the modified NEUROD1 protein is about 45-50 kDa.

Publications

Species	Application	Title
human	WB	Sci Adv; Prototypical oncogene family Myc defines unappreciated distinct lineage states of small cell lung cancer.; Authors - Ayushi S Patel
mouse	IF	Sci Adv; Neurons burdened by DNA double-strand breaks incite microglia activation through antiviral-like signaling in neurodegeneration; Authors - Gwyneth M Welch
mouse	WB	Biomaterials; Three-dimensional cell-culture platform based on hydrogel with tunable microenvironmental properties to improve insulin-secreting function of MIN6 cells.; Authors - Miao Zhang
mouse	WB	Proc Natl Acad Sci U S A; CHD8 dosage regulates transcription in pluripotency and early murine neural differentiation.; Authors - Sabina Sood
mouse	IHC	Cell Rep; PMID: 31189116; Authors - Franziska Böttger
mouse	IF	PLoS Biol; Centrioles are amplified in cycling progenitors of olfactory sensory neurons.; Authors - Kaitlin Ching

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tanusree (Verified Customer) (12-03-2019)

This antibody works good in western blotting analysis using mouse tissues.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse brain