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NEUROD1 Polyclonal antibody
NEUROD1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
human, mouse, rat
WB, IP, IHC, IF, ELISA
Cat no : 12081-1-AP
Validation Data Gallery
|Positive WB detected in||Y79 cells|
|Positive IP detected in||Y79 cells|
|Positive IHC detected in||rat brain tissue, human pancreas cancer tissue, mouse brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||mouse brain tissue|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
|KD/KO||See 1 publications below|
|WB||See 7 publications below|
|IHC||See 4 publications below|
|IF||See 2 publications below|
12081-1-AP targets NEUROD1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||NEUROD1 fusion protein Ag2713|
|Full Name||neurogenic differentiation 1|
|Calculated molecular weight||356 aa, 40 kDa|
|Observed molecular weight||50 kDa|
|GenBank accession number||BC009046|
|Gene ID (NCBI)||4760|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
NeuroD is a member of the basic helix-loop-helix (bHLH) family of transcription factors. The basic helix-loop-helix (bHLH) proteins are transcription factors that are required for several aspects of development, including cell type determination, terminal differentiation and sex determination. Members of the myogenic determination family, MyoD, myf5, myogenin and MRF4, all have bHLH domains.These proteins function by forming heterodimers with E-proteins and binding to the canonical E-box sequence CANNTG. Neuro D is expressed transiently in a subset of neurons in the central and peripheral nervous systems at the time of their terminal differentiation into mature neurons. Moreover, ectopic expression of Neuro D in Xenopus embryos induces premature differentiation of neuronal precursors and Neuro D can convert presumptive epidermal cells into neurons.The lack of NeuroD in the brain results in severe defects in development. Human mutations have been linked to a number of types of diabetes including type I diabetes mellitus and maturity-onset diabetes of the young. The calculated molecular weight of NEUROD1 is 39 kDa, but the modified NEUROD1 protein is about 45-50 kDa.
|Product Specific Protocols|
|WB protocol for NEUROD1 antibody 12081-1-AP||Download protocol|
|IHC protocol for NEUROD1 antibody 12081-1-AP||Download protocol|
|IF protocol for NEUROD1 antibody 12081-1-AP||Download protocol|
|IP protocol for NEUROD1 antibody 12081-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Prototypical oncogene family Myc defines unappreciated distinct lineage states of small cell lung cancer.
Neurons burdened by DNA double-strand breaks incite microglia activation through antiviral-like signaling in neurodegeneration
Three-dimensional cell-culture platform based on hydrogel with tunable microenvironmental properties to improve insulin-secreting function of MIN6 cells.
Proc Natl Acad Sci U S A
CHD8 dosage regulates transcription in pluripotency and early murine neural differentiation.
Centrioles are amplified in cycling progenitors of olfactory sensory neurons.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Tanusree (Verified Customer) (12-03-2019)
This antibody works good in western blotting analysis using mouse tissues.