|Positive WB detected in||Jurkat cells, Sp2/0 cells|
|Positive IP detected in||Jurkat cells|
|Positive IHC detected in||human testis tissue, human stomach tissue, mouse brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
The immunogen of 22023-1-AP is NFATC2 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||NFATC2 fusion protein Ag16849|
|Full Name||nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2|
|Calculated molecular weight||925 aa, 100 kDa|
|Observed molecular weight||135 kDa|
|GenBank accession number||BC136418|
|Gene ID (NCBI)||4773|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Nuclear factor of activated T-cells, cytoplasmic 2 (NFATC2), also named NFAT1, or NFATP, is a 925 amino acid protein, which is expressed in thymus, spleen, heart, testis, brain, placenta, muscle and pancreas. Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals. The subcellular localization of NFATC plays a key role in the regulation of gene transcription. NFATC2 plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2, IL-3, IL-4, TNF-alpha or GM-CSF. NFATC2 promotes invasive migration through the activation of GPC6 expression and WNT5A signaling pathway. The calculated molecular weight of NFATC2 is about 97-100 kDa, but the modified NFATC2 protein is about 135 kDa.
|Product Specific Protocols|
|WB protocol for NFATC2 antibody 22023-1-AP||Download protocol|
|IHC protocol for NFATC2 antibody 22023-1-AP||Download protocol|
|IF protocol for NFATC2 antibody 22023-1-AP||Download protocol|
|IP protocol for NFATC2 antibody 22023-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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