NFATC2 Polyclonal antibody

• Featured Product
• KD/KO Validated

NFATC2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Publications

22

Cat no : 22023-1-AP

Synonyms

NFAT1, NFATP, NF-ATc2, NFAT pre-existing subunit, NFAT pre existing subunit

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Validation Data Gallery

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  WB analysis of Jurkat using 22023-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 22023-1-AP (NFATC2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Sp2/0 using 22023-1-AP

  Sp2/0 cells were subjected to SDS PAGE followed by western blot with 22023-1-AP (NFATC2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Jurkat using 22023-1-AP

  IP result of anti-NFATC2 (IP:22023-1-AP, 4ug; Detection:22023-1-AP 1:1000) with Jurkat cells lysate 3200ug.

• 

  IHC staining of human breast cancer using 22023-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22023-1-AP (NFATC2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 22023-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22023-1-AP (NFATC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 22023-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22023-1-AP (NFATC2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 22023-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22023-1-AP (NFATC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 22023-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 22023-1-AP (NFATC2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 22023-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 22023-1-AP (NFATC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of U-251 using 22023-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U-251 cells using NFATC2 antibody (22023-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of Jurkat using 22023-1-AP

  1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human NFATC2 (22023-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: Jurkat cells
• Positive IP detected in: Jurkat cells
• Positive IHC detected in: human breast cancer tissue, human colon cancer tissue, human lymphoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: U-251 cells
• Positive FC (Intra) detected in: Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 15 publications below
• IHC: See 3 publications below
• IF: See 10 publications below

Product Information

22023-1-AP targets NFATC2 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NFATC2 fusion protein Ag16849
• Full Name: nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2
• Calculated Molecular Weight: 925 aa, 100 kDa
• Observed Molecular Weight: 135 kDa
• GenBank Accession Number: BC136418
• Gene Symbol: NFATC2
• Gene ID (NCBI): 4773
• RRID: AB_2878973
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Nuclear factor of activated T-cells, cytoplasmic 2 (NFATC2), also named NFAT1, or NFATP, is a 925 amino acid protein, which is expressed in thymus, spleen, heart, testis, brain, placenta, muscle and pancreas. Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals. The subcellular localization of NFATC plays a key role in the regulation of gene transcription. NFATC2 plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2, IL-3, IL-4, TNF-alpha or GM-CSF. NFATC2 promotes invasive migration through the activation of GPC6 expression and WNT5A signaling pathway. The calculated molecular weight of NFATC2 is about 97-100 kDa, but the modified NFATC2 protein is about 135 kDa.

Publications

Species	Application	Title
mouse	WB, IF	Biomaterials; Targeted regulation of lymphocytic ER stress response with an overall immunosuppression to alleviate allograft rejection.; Authors - Yingying Shi
human	WB,IF	Breast Cancer Res; Calcium-sensing stromal interaction molecule 2 upregulates nuclear factor of activated T cells 1 and transforming growth factor-β signaling to promote breast cancer metastasis.; Authors - Yutian Miao ; KD Validated
human	WB,IHC	Cell Mol Gastroenterol Hepatol; ANKRD22 Drives Rapid Proliferation of Lgr5+ Cells and Acts as a Promising Therapeutic Target in Gastric Mucosal Injury.; Authors - Jingwen Liu
	IF	Mucosal Immunol; IL-33/ST2/IL-9/IL-9R signaling disrupts ocular surface barrier in allergic inflammation.; Authors - Jiaoyue Hu
mouse	WB	J Neuroinflammation; Human PMSCs-derived small extracellular vesicles alleviate neuropathic pain through miR-26a-5p/Wnt5a in SNI mice model; Authors - Yitian Lu
mouse	WB	CNS Neurosci Ther; miR-26a-5p alleviates CFA-induced chronic inflammatory hyperalgesia through Wnt5a/CaMKII/NFAT signaling in mice; Authors - Yitian Lu