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  • KD/KO Validated

NFATC2 Polyclonal antibody

NFATC2 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 22023-1-AP

Synonyms

NF ATc2, NF ATp, NFAT pre existing subunit, NFAT1, NFATC2, NFATP



Tested Applications

Positive WB detected inJurkat cells, Sp2/0 cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman testis tissue, human stomach tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 22023-1-AP is NFATC2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NFATC2 fusion protein Ag16849
Full Name nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2
Calculated molecular weight 925 aa, 100 kDa
Observed molecular weight 135 kDa
GenBank accession numberBC136418
Gene symbol NFATC2
Gene ID (NCBI) 4773
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Nuclear factor of activated T-cells, cytoplasmic 2 (NFATC2), also named NFAT1, or NFATP, is a 925 amino acid protein, which is expressed in thymus, spleen, heart, testis, brain, placenta, muscle and pancreas. Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals. The subcellular localization of NFATC plays a key role in the regulation of gene transcription. NFATC2 plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2, IL-3, IL-4, TNF-alpha or GM-CSF. NFATC2 promotes invasive migration through the activation of GPC6 expression and WNT5A signaling pathway. The calculated molecular weight of NFATC2 is about 97-100 kDa, but the modified NFATC2 protein is about 135 kDa.

Protocols

Product Specific Protocols
WB protocol for NFATC2 antibody 22023-1-APDownload protocol
IHC protocol for NFATC2 antibody 22023-1-APDownload protocol
IF protocol for NFATC2 antibody 22023-1-APDownload protocol
IP protocol for NFATC2 antibody 22023-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Breast Cancer Res

Calcium-sensing stromal interaction molecule 2 upregulates nuclear factor of activated T cells 1 and transforming growth factor-β signaling to promote breast cancer metastasis.

Authors - Yutian Miao
  • KD Validated
mouseIF

J Diabetes Res

Protective Role of Tangshen Formula on the Progression of Renal Damage in db/db Mice by TRPC6/Talin1 Pathway in Podocytes.

Authors - Qian Wang
humanWB

Reprod Sci

NFAT Overexpression Correlates with CA72-4 and Poor Prognosis of Ovarian Clear-Cell Carcinoma Subtype.

Authors - Bing Xin
humanWB

Biochem Biophys Res Commun

Inhibition of MCF-7 breast cancer cell proliferation by a synthetic peptide derived from the C-terminal sequence of Orai channel.

Authors - Shan Li
humanIF

Life Sci

Inhibition of RELM-β prevents hypoxia-induced overproliferation of human pulmonary artery smooth muscle cells by reversing PLC-mediated KCNK3 decline.

Authors - Linlin Han
human,mouseWB

Liver Int

Disruption of Plin5 degradation by CMA causes lipid homeostasis imbalance in NAFLD.

Authors - Shuo Y Ma