NFATC3 Polyclonal antibody

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  WB analysis using 18222-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18222-1-AP (NFATC3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 18222-1-AP

  IP result of anti-NFATC3 (IP:18222-1-AP, 4ug; Detection:18222-1-AP 1:1000) with K-562 cells lysate 1760 ug.

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  IHC staining of human ovary using 18222-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 18222-1-AP (NFATC3 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human testis using 18222-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18222-1-AP (NFATC3 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 18222-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18222-1-AP (NFATC3 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human ovary using 18222-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 18222-1-AP (NFATC3 antibody) at dilution of 1:100 (under 10x lens).

• Featured Product
• KD/KO Validated

NFATC3 Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 18222-1-AP

Publications(31)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF, IP, chIP, ELISA

Synonyms

NF AT4, NF ATc3, NFAT4, NFATC3, NFATX

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Tested Applications

• Positive WB detected in: Jurkat cells, K-562 cells
• Positive IP detected in: K-562 cells
• Positive IHC detected in: human ovary tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 22 publications below
• IHC: See 2 publications below
• IF: See 7 publications below
• IP: See 1 publications below
• ChIP: See 4 publications below

Product Information

18222-1-AP targets NFATC3 in WB, IHC, IF, IP, chIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NFATC3 fusion protein Ag12985
• Full Name: nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 3
• Calculated Molecular Weight: 116 kDa
• Observed Molecular Weight: 130-170 kDa
• GenBank Accession Number: BC001050
• Gene Symbol: NFATC3
• Gene ID (NCBI): 4775
• RRID: AB_2152773
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q12968
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NFAT (nuclear factors of activated T cells) proteins are a family of transcription factors originally identified as mediators of activation of cytokine genes in response to antigenic stimulation of T cells. NFAT proteins also play varied roles in cells outside of the immune system [PMID:11877454]. NFATc3 has specifically been implicated in vasculature development, regulation of smooth muscle contractile phenotype, and modulation of vascular smooth muscle contractility [PMID:11439183,17148444]. The calculated molecular weight of NFATC3 is 115 kDa, but the post-modified protein is about 130-170 kDa (PMID: 15728531, PMID: 15857835)

Publications

Species	Application	Title
	ChIP	Immunity; Metformin inhibition of mitochondrial ATP and DNA synthesis abrogates NLRP3 inflammasome activation and pulmonary inflammation.; Authors - Hongxu Xian
human	WB	Gastroenterology; Regulator of Calcineurin 1 Gene Isoform 4, Downregulated in Hepatocellular Carcinoma, Prevents Proliferation, Migration, and Invasive Activity of Cancer Cells and Growth of Orthotopic Tumors by Inhibiting Nuclear Translocation of NFAT1.; Authors - Haojie Jin
mouse	WB,IP	Cell Death Differ; Trim39 regulates neuronal apoptosis by acting as a SUMO-targeted E3 ubiquitin-ligase for the transcription factor NFATc3.; Authors - Meenakshi Basu-Shrivastava
human	WB	Oncogene; Regulator of calcineurin 1 gene isoform 4 in pancreatic ductal adenocarcinoma regulates the progression of tumor cells.; Authors - Mengyi Lao
human	WB	Cancer Lett; TRPC3 promotes tumorigenesis of gastric cancer via the CNB2/GSK3β/NFATc2 signaling pathway.; Authors - Da-Cen Lin
human	WB	Cell Biol Toxicol; HNRNPA2B1 stabilizes NFATC3 levels to potentiate its combined actions with FOSL1 to mediate vasculogenic mimicry in GBM cells; Authors - Hanting Wang