NFATC3 Polyclonal antibody

• Featured Product
• KD/KO Validated

NFATC3 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IHC, IF, chIP, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 18222-1-AP

Synonyms

NF AT4, NF ATc3, NFAT4, NFATC3, NFATX

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Validation Data Gallery

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  WB analysis of mouse thymus using 18222-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 18222-1-AP (NFATC3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary using 18222-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 18222-1-AP (NFATC3 antibody) at dilution of 1:100 (under 40x lens).

• 

  IP experiment of mouse skeletal muscle using 18222-1-AP

  IP Result of anti-NFATC3 (IP:18222-1-AP, 5ug; Detection:18222-1-AP 1:500) with mouse skeletal muscle tissue lysate 2000ug.

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Tested Applications

• Positive WB detected in: mouse thymus tissue, mouse skeletal muscle tissue
• Positive IP detected in: mouse skeletal muscle tissue
• Positive IHC detected in: human ovary tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• ChIP: See 1 publications below

Product Information

18222-1-AP targets NFATC3 in WB, IP, IHC, IF, chIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NFATC3 fusion protein Ag12985
• Full Name: nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 3
• Calculated molecular weight: 116 kDa
• Observed molecular weight: 130-170 kDa
• GenBank accession number: BC001050
• Gene symbol: NFATC3
• Gene ID (NCBI): 4775
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

NFAT (nuclear factors of activated T cells) proteins are a family of transcription factors originally identified as mediators of activation of cytokine genes in response to antigenic stimulation of T cells. NFAT proteins also play varied roles in cells outside of the immune system [PMID:11877454]. NFATc3 has specifically been implicated in vasculature development, regulation of smooth muscle contractile phenotype, and modulation of vascular smooth muscle contractility [PMID:11439183,17148444]. The calculated molecular weight of NFATC3 is 115 kDa, but the post-modified protein is about 130-170 kDa (PMID: 15728531, PMID: 15857835)

Publications

Species	Application	Title
mouse	IHC	JBMR Plus; Methionine Enkephalin Suppresses Osteocyte Apoptosis Induced by Compressive Force through Regulation of Nuclear Translocation of NFATc1.; Authors - Chisumi Sogi
mouse	WB	Eur J Pharmacol; Stachydrine hydrochloride suppresses phenylephrine-induced pathological cardiac hypertrophy by inhibiting the calcineurin/nuclear factor of activated T-cell signalling pathway.; Authors - Jiali Zheng
mouse	WB	Cell Signal; Blocking NFATc3 ameliorates azoxymethane/dextran sulfate sodium induced colitis-associated colorectal cancer in mice via the inhibition of inflammatory responses and epithelial-mesenchymal transition.; Authors - Yan Lin
human	WB,chIP	Cell Commun Signal; TRPV2-induced Ca2+-calcineurin-NFAT signaling regulates differentiation of osteoclast in multiple myeloma.; Authors - Hua Bai
human	WB	Mol Biol Cell; Nuclear factor of activated T-cell c3 inhibition of mammalian target of rapamycin signaling through induction of regulated in development and DNA damage response 1 in human intestinal cells.; Authors - Zhou Yuning Y ; KD Validated
rat	IF	Cell Physiol Biochem; Calcineurin/NFAT Signaling Modulates Pulmonary Artery Smooth Muscle Cell Proliferation, Migration and Apoptosis in Monocrotaline-Induced Pulmonary Arterial Hypertension Rats.; Authors - Rui-Lan He