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NFATC3 Polyclonal antibody
NFATC3 Polyclonal Antibody for IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
WB, IP, IHC, IF, chIP,ELISA
Conjugate
Unconjugated
Cat no : 18222-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse thymus tissue, mouse skeletal muscle tissue |
Positive IP detected in | mouse skeletal muscle tissue |
Positive IHC detected in | human ovary tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:200-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 2 publications below |
WB | See 9 publications below |
IHC | See 1 publications below |
IF | See 3 publications below |
ChIP | See 1 publications below |
Product Information
18222-1-AP targets NFATC3 in WB, IP, IHC, IF, chIP,ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | NFATC3 fusion protein Ag12985 |
Full Name | nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 3 |
Calculated molecular weight | 116 kDa |
Observed molecular weight | 130-170 kDa |
GenBank accession number | BC001050 |
Gene symbol | NFATC3 |
Gene ID (NCBI) | 4775 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
NFAT (nuclear factors of activated T cells) proteins are a family of transcription factors originally identified as mediators of activation of cytokine genes in response to antigenic stimulation of T cells. NFAT proteins also play varied roles in cells outside of the immune system [PMID:11877454]. NFATc3 has specifically been implicated in vasculature development, regulation of smooth muscle contractile phenotype, and modulation of vascular smooth muscle contractility [PMID:11439183,17148444]. The calculated molecular weight of NFATC3 is 115 kDa, but the post-modified protein is about 130-170 kDa (PMID: 15728531, PMID: 15857835)
Protocols
Product Specific Protocols | |
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WB protocol for NFATC3 antibody 18222-1-AP | Download protocol |
IHC protocol for NFATC3 antibody 18222-1-AP | Download protocol |
IP protocol for NFATC3 antibody 18222-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
J Diabetes Res Protective Role of Tangshen Formula on the Progression of Renal Damage in db/db Mice by TRPC6/Talin1 Pathway in Podocytes. | ||
JBMR Plus Methionine Enkephalin Suppresses Osteocyte Apoptosis Induced by Compressive Force through Regulation of Nuclear Translocation of NFATc1. | ||
Eur J Pharmacol Stachydrine hydrochloride suppresses phenylephrine-induced pathological cardiac hypertrophy by inhibiting the calcineurin/nuclear factor of activated T-cell signalling pathway. | ||
Cell Signal Blocking NFATc3 ameliorates azoxymethane/dextran sulfate sodium induced colitis-associated colorectal cancer in mice via the inhibition of inflammatory responses and epithelial-mesenchymal transition. | ||
Biochim Biophys Acta Mol Cell Res The calcium pump PMCA4 prevents epithelial-mesenchymal transition by inhibiting NFATc1-ZEB1 pathway in gastric cancer. | ||
Front Pharmacol Tsantan Sumtang Restored Right Ventricular Function in Chronic Hypoxia-Induced Pulmonary Hypertension Rats. |