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NFATC3 Antibody 4 Publications

Rabbit Polyclonal| Catalog number: 18222-1-AP

Featured Product KD/KO validated

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Con: 51 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, chIP, ELISA
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Images See all

NFATC3 Antibody WB mouse thymus tissue 18222-1-AP

mouse thymus tissue were subjected to SDS PAGE followed by western blot with 18222-1-AP( NFATC3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours

NFATC3 Antibody IHC human ovary tissue 18222-1-AP

Immunohistochemical of paraffin-embedded human ovary using 18222-1-AP(NFATC3 antibody) at dilution of 1:100 (under 40x lens)

NFATC3 Antibody IP mouse skeletal muscle tissue 18222-1-AP

IP Result of anti-NFATC3 (IP:18222-1-AP, 5ug; Detection:18222-1-AP 1:500) with mouse skeletal muscle tissue lysate 2000ug.

Species specificity:
human, mouse, rat
Positive WB detected in:
mouse thymus tissue, mouse skeletal muscle tissue
Positive IP detected in:
mouse skeletal muscle tissue
Positive IHC detected in:
human ovary tissue, human testis tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution:
WB : 1:200-1:1000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:20-1:200

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
NFATC3 fusion protein Ag12985
Full name:
nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 3
Calculated molecular weight:
116 kDa
Observed molecular weight:
130-170 kDa
GenBank accession number:
BC001050
Gene ID (NCBI):
4775
Gene symbol
NFATC3
Synonyms
NF AT4, NF ATc3, NFAT4, NFATC3, NFATX

Background

NFAT (nuclear factors of activated T cells) proteins are a family of transcription factors originally identified as mediators of activation of cytokine genes in response to antigenic stimulation of T cells. NFAT proteins also play varied roles in cells outside of the immune system [PMID:11877454]. NFATc3 has specifically been implicated in vasculature development, regulation of smooth muscle contractile phenotype, and modulation of vascular smooth muscle contractility [PMID:11439183,17148444]. The calculated molecular weight of NFATC3 is 115 kDa, but the post-modified protein is about 130-170 kDa (PMID: 15728531, PMID: 15857835)


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