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- KD/KO Validated
NRF2, NFE2L2 Polyclonal antibody
NRF2, NFE2L2 Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA
Cat no : 16396-1-AP
|Positive WB detected in||HeLa cells, DMSO treated HeLa cells, rat liver tissue|
|Positive IP detected in||mouse kidney tissue|
|Positive IHC detected in||human ovary tumor tissue, human breast cancer tissue, human colon cancer tissue, human kidney tissue, human liver cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Positive FC detected in||MCF-7 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
16396-1-AP targets NRF2, NFE2L2 in WB, IP, IHC, IF, FC, CoIP, ChIP,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||Bovine, chicken, Codonopsis lanceolata, hamster, human, monkey, mouse, pig, rat, Seiurus aurocapilla|
|Host / Isotype||Rabbit / IgG|
|Immunogen||NRF2, NFE2L2 fusion protein Ag9489|
|Full Name||nuclear factor (erythroid-derived 2)-like 2|
|Calculated molecular weight||605 aa, 68 kDa|
|Observed molecular weight||110 kDa, 68 kDa|
|GenBank accession number||BC011558|
|Gene ID (NCBI)||4780|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
NRF2, also named as NFE2L2, belongs to the bZIP family and CNC subfamily. It is a transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. NRF2 is important for the coordinated up-regulation of genes in response to oxidative stress. It may be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region.Nrf2 is a key player in the regulation of genes encoding for many antioxidative response enzymes.The expression of NRF2 may be induced under oxidative stress (PMID:14567983).In lung cancer, Nrf2 activation in malignant cells has been associated with tumor progression and chemotherapy resistance(PMID:20534738). Identifying patients with abnormal NRF2 expression may be important for selection for chemotherapy in NSCLC. As new investigators break into the emerging field of Nrf2 research, confusion regarding the correct migratory pattern of Nrf2 is causing doubts about the accuracy and reproducibility of published results. This letter provides solid evidence that the biologically relevant molecular weight of Nrf2 is ~95-110 kDa and not the predicted ~55-65 kDa based on its 2-kilobase open reading frame. The data discussed and presented here will hopefully lead to a uniform acceptance that future experiments and publications should be designed around detecting Nrf2 at the correct molecular weight of ~95-120 kDa (PMID: 22703241).
|Product Specific Protocols|
|WB protocol for NRF2, NFE2L2 antibody 16396-1-AP||Download protocol|
|IHC protocol for NRF2, NFE2L2 antibody 16396-1-AP||Download protocol|
|IF protocol for NRF2, NFE2L2 antibody 16396-1-AP||Download protocol|
|IP protocol for NRF2, NFE2L2 antibody 16396-1-AP||Download protocol|
|FC protocol for NRF2, NFE2L2 antibody 16396-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Razia Sultana (Verified Customer) (08-20-2020)
The antibody gives good band at around 100KD(100 KD in HK2 cells and 90KD in kidney tissue lysate) along with some non specific bands at around 40-50KD. The trial size gave a band at 130KD which is unusual and lot of non specific bands,but the full size antibody is good. We are satisfied with the purchase
JIM (Verified Customer) (03-26-2019)
Strong Nrf2 signal can be observed in macrophages treated with LPS and D3T by western blots.