• Featured Product
  • KD/KO Validated

NRF2, NFE2L2 Antibody

NRF2, NFE2L2 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (8)

Applications

FC, IF, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Publications

260

Cat no : 16396-1-AP

Synonyms

HEBP1, NF E2 related factor 2, NFE2 related factor 2, NFE2L2, NRF2



Tested Applications

Positive WB detected inHeLa cells, DMSO treated HeLa cells, rat liver tissue
Positive IP detected inmouse kidney tissue
Positive IHC detected inhuman ovary tumor tissue, human breast cancer tissue, human colon cancer tissue, human kidney tissue, human liver cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

16396-1-AP targets NRF2, NFE2L2 in FC, IF, IHC, IP, WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Reactivity human, mouse, rat
Cited Species African green monkey, bird, Bovine, calf, Codonopsis lanceolata, hamsters, human, mouse, ostrich chick, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NRF2, NFE2L2 fusion protein Ag9489
Full Name nuclear factor (erythroid-derived 2)-like 2
Calculated molecular weight 605aa,68 kDa
Observed molecular weight 110, 68 kDa
GenBank accession number BC011558
Gene symbol NFE2L2
Gene ID (NCBI) 4780
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20oC. Aliquoting is unnecessary for -20oC storage.

Background Information

NRF2, also named as NFE2L2, belongs to the bZIP family and CNC subfamily. It is a transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. NRF2 is important for the coordinated up-regulation of genes in response to oxidative stress. It may be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region.Nrf2 is a key player in the regulation of genes encoding for many antioxidative response enzymes.The expression of NRF2 may be induced under oxidative stress (PMID:14567983).In lung cancer, Nrf2 activation in malignant cells has been associated with tumor progression and chemotherapy resistance(PMID:20534738). Identifying patients with abnormal NRF2 expression may be important for selection for chemotherapy in NSCLC. As new investigators break into the emerging field of Nrf2 research, confusion regarding the correct migratory pattern of Nrf2 is causing doubts about the accuracy and reproducibility of published results. This letter provides solid evidence that the biologically relevant molecular weight of Nrf2 is ~95-110 kDa and not the predicted ~55-65 kDa based on its 2-kilobase open reading frame. The data discussed and presented here will hopefully lead to a uniform acceptance that future experiments and publications should be designed around detecting Nrf2 at the correct molecular weight of ~95-120 kDa (PMID: 22703241).

Protocols

Product Specific Protocols
WB protocol for NRF2, NFE2L2 antibody 16396-1-APDownload protocol
IHC protocol for NRF2, NFE2L2 antibody 16396-1-APDownload protocol
IF protocol for NRF2, NFE2L2 antibody 16396-1-APDownload protocol
IP protocol for NRF2, NFE2L2 antibody 16396-1-APDownload protocol
FC protocol for NRF2, NFE2L2 antibody 16396-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Mol Nutr Food Res

Sulforaphane Improves Lipid Metabolism by Enhancing Mitochondrial Function and Biogenesis in vivo and vitro.

Authors - Peng Lei
ratWB,IHC

Toxicol Appl Pharmacol

Dexmedetomidine attenuates lipopolysaccharide-induced liver oxidative stress and cell apoptosis in rats by increasing GSK-3β/MKP-1/Nrf2 pathway activity via the α2 adrenergic receptor.

Authors - Jichen Sha
ratWB,IHC

Toxicol Appl Pharmacol

Dexmedetomidine attenuates lipopolysaccharide-induced liver oxidative stress and cell apoptosis in rats by increasing GSK-3β/MKP-1/Nrf2 pathway activity via the α2 adrenergic receptor.

Authors - Jichen Sha
ratWB

Reprod Toxicol

N-acetylcysteine alleviates fluoride-induced testicular apoptosis by modulating IRE1α/JNK signaling and nuclear Nrf2 activation.

Authors - Yazhen Hu
mouseWB

Int Immunopharmacol

Hepatoprotective effects and structure-activity relationship of five flavonoids against lipopolysaccharide/d-galactosamine induced acute liver failure in mice.

Authors - Yutong He
ratWB

Transl Neurosci

Fangchinoline Ameliorates the Expressions of Angiogenic Molecule in Cerebral Ischemia Induced Neuronal Degeneration in Neonatal Rats.

Authors - Han Daicheng

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

JIM YEN (03-26-2019)

Strong Nrf2 signal can be observed in macrophages treated with LPS and D3T by western blots.

  • Applications: Western Blot,
No image submitted with review