• Featured Product
  • KD/KO Validated

NRF2, NFE2L2 Polyclonal antibody

NRF2, NFE2L2 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (8)

Applications

WB, IP, IHC, IF, FC, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 16396-1-AP

Synonyms

HEBP1, NF E2 related factor 2, NFE2 related factor 2, NFE2L2, NRF2, NRF2, NFE2L2



Tested Applications

Positive WB detected inA549 cells, DMSO treated HeLa cells, rat liver tissue, HepG2 cells
Positive IP detected inmouse kidney tissue
Positive IHC detected inhuman ovary tumor tissue, human breast cancer tissue, human colon cancer tissue, human kidney tissue, human liver cancer tissue, human renal cell carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

16396-1-AP targets NRF2, NFE2L2 in WB, IP, IHC, IF, FC, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, Bovine, canine, chicken, ducks, hamster, monkey, pig, rabbit
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NRF2, NFE2L2 fusion protein Ag9489
Full Name nuclear factor (erythroid-derived 2)-like 2
Calculated molecular weight 605 aa, 68 kDa
Observed molecular weight 110 kDa, 68 kDa
GenBank accession numberBC011558
Gene symbol NFE2L2
Gene ID (NCBI) 4780
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NRF2, also named as NFE2L2, belongs to the bZIP family and CNC subfamily. It is a transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. NRF2 is important for the coordinated up-regulation of genes in response to oxidative stress. It may be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region. Nrf2 is a key player in the regulation of genes encoding for many antioxidative response enzymes.The expression of NRF2 may be induced under oxidative stress (PMID:14567983).In lung cancer, Nrf2 activation in malignant cells has been associated with tumor progression and chemotherapy resistance(PMID:20534738). Identifying patients with abnormal NRF2 expression may be important for selection for chemotherapy in NSCLC. As new investigators break into the emerging field of Nrf2 research, confusion regarding the correct migratory pattern of Nrf2 is causing doubts about the accuracy and reproducibility of published results. This letter provides solid evidence that the actually observed molecular weight of Nrf2 is about 70kDa and 95-110 kDa. (PMID: 22703241).

Protocols

Product Specific Protocols
WB protocol for NRF2, NFE2L2 antibody 16396-1-APDownload protocol
IHC protocol for NRF2, NFE2L2 antibody 16396-1-APDownload protocol
IF protocol for NRF2, NFE2L2 antibody 16396-1-APDownload protocol
IP protocol for NRF2, NFE2L2 antibody 16396-1-APDownload protocol
FC protocol for NRF2, NFE2L2 antibody 16396-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IF

Nature

A plant-derived natural photosynthetic system for improving cell anabolism

Authors - Pengfei Chen
humanWB

Nature

Structure of the nutrient-sensing hub GATOR2.

Authors - Max L Valenstein
humanWB, IP

Cancer Cell

iASPP Is an Antioxidative Factor and Drives Cancer Growth and Drug Resistance by Competing with Nrf2 for Keap1 Binding.

Authors - Wenjie Ge
  • KD Validated
human

Cell Metab

Transsulfuration Activity Can Support Cell Growth upon Extracellular Cysteine Limitation.

Authors - Jiajun Zhu
mouseWB

Cell Metab

Exposure to Static Magnetic and Electric Fields Treats Type 2 Diabetes.

Authors - Calvin S Carter
humanWB

Nat Cell Biol

Mitochondrial fission links ECM mechanotransduction to metabolic redox homeostasis and metastatic chemotherapy resistance.

Authors - Patrizia Romani
  • KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Guillermo (Verified Customer) (09-03-2022)

Very good product, great spots.

  • Applications: Western Blot
FH

balawant (Verified Customer) (08-06-2022)

for westerblotting I have used this Antibody. this antibody is excellent and I will recommend if you are working with this protein

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:4000
  • Cell Tissue Type: colon tissue and colon cancer cells
FH

Georgina (Verified Customer) (07-04-2022)

This is a good antibody - it gives some background signal but the band of interest is clear. Sometimes need to use a higher concentration than recommended for cells with lower expression of NRF2.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1 in 500 or 1 in 1000
  • Cell Tissue Type: Human HepG2 cells
FH

Iram (Verified Customer) (03-11-2022)

Gave good bands around 100 kda in colon cancer cell lysates

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: Colon cancer cells
FH

Giovanni (Verified Customer) (01-11-2022)

the antiboby worked very well at dilution 1:1000 in WB using Htr8/SVneo cell line lysates. It gave a specific band at around 100 kDa with very low background.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Htr8/SVneo
NRF2, NFE2L2 Antibody Western Blot validation (1:1000 dilution) in Htr8/SVneo (Cat no:16396-1-AP)
FH

Xiaoping (Verified Customer) (10-14-2021)

Gave good bands around 100 kda in renal cell lysates

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:800
  • Cell Tissue Type: Kidney
FH

Razia Sultana (Verified Customer) (08-20-2020)

The antibody gives good band at around 100KD(100 KD in HK2 cells and 90KD in kidney tissue lysate) along with some non specific bands at around 40-50KD. The trial size gave a band at 130KD which is unusual and lot of non specific bands,but the full size antibody is good. We are satisfied with the purchase

  • Applications: Western Blot, Immunohistochemistry
  • Primary Antibody Dilution: 1:1000 in 5% milk
  • Cell Tissue Type: Rat and mouse kidney tissue lysate and HK2 cell lysate
FH

JIM (Verified Customer) (03-26-2019)

Strong Nrf2 signal can be observed in macrophages treated with LPS and D3T by western blots.

  • Applications: Western Blot,