NFKB1 Polyclonal antibody

NFKB1 Polyclonal Antibody for IF, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat and More (4)

Applications

WB, RIP, IP, IHC, IF, ChIP

Conjugate

Unconjugated

Publications

126

Cat no : 14220-1-AP

Synonyms

DNA binding factor KBF1, EBP 1, KBF1, NF kappa B, NFKB p105, NFKB p50, NFKB1, NFKB1,p105, NFKB1,p105,p50, nfkb1a, NF-κB, NF-κB 1, p105, p50

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Validation Data Gallery

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  IF Staining of HepG2 using 14220-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 14220-1-AP (NF-κB antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  WB analysis of HeLa using 14220-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14220-1-AP (NF-κB antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 14220-1-AP

  IP Result of anti-NF-κB (IP:14220-1-AP, 3ug; Detection:14220-1-AP 1:500) with Jurkat cells lysate 1800ug.

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Tested Applications

• Positive WB detected in: HeLa cells, Jurkat cells
• Positive IP detected in: Jurkat cells
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 110 publications below
• IHC: See 8 publications below
• IF: See 15 publications below
• IP: See 3 publications below
• ChIP: See 1 publications below
• RIP: See 1 publications below

Product Information

14220-1-AP targets NFKB1 in WB, RIP, IP, IHC, IF, ChIP applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Cited Reactivity: bovine, CHICKEN, ducks, human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NFKB1 fusion protein Ag5458
• Full Name: nuclear factor of kappa light polypeptide gene enhancer in B-cells 1
• Calculated molecular weight: 105 kDa
• Observed molecular weight: 50 kDa and 105 kDa
• GenBank accession number: BC051765
• Gene symbol: NFKB1
• Gene ID (NCBI): 4790
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

NFkB is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NFkB is activated by various intra- and extracellular stimuli such as cytokines, oxidant free radicals, ultraviolet irradiation, and bacterial or viral products. NFkB is a family of transcription factors that consists of homo- and heterodimers of NFkB1/p50 and RelA/p65 subunits, and controls a variety of cellular events including development and immune responses. All members share a conserved amino terminus domain that includes dimerization, nuclear localization, and DNA binding regions, and a carboxy terminal transactivation domain. Serines 529 and 536 in the transactivation domain of RelA/p65 are phosphorylated in response to several stimuli including phorbol ester, IL1 alpha and TNF alpha as mediated by IkB kinase and p38 MAPK. Phosphorylation of serines 529 and 536 is critical for RelA/p65 transcriptional activity. Activated NFkB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFkB has been associated with a number of inflammatory diseases while persistent inhibition of NFkB leads to inappropriate immune cell development or delayed cell growth. NFKB1 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p105 and generation of p50 by a cotranslational processing. This antibody can bind both p105 and p50 isoforms of NFKB1.

Publications

Species	Application	Title
human	WB	Food Nutr Res; Pretreatment with Lactobacillus reuteri F-9-35 attenuates ethanol-induced gastric injury in rats.; Authors - Mao-Cheng Sun
rat	WB	Iran J Basic Med Sci; Tempol relieves lung injury in a rat model of chronic intermittent hypoxia via suppression of inflammation and oxidative stress.; Authors - Yeying Wang
human	WB	Molecules; Effects of Betaine on LPS-Stimulated Activation of Microglial M1/M2 Phenotypes by Suppressing TLR4/NF-κB Pathways in N9 Cells.; Authors - Hui Shi
bovine	WB	J Appl Toxicol; Pharmacological inhibition of TLR4/NF-κB with TLR4-IN-C34 attenuated microcystin-leucine arginine toxicity in bovine Sertoli cells.; Authors - Elikanah Olusayo Adegoke
human	WB	Oncol Lett; Paeonol-mediated apoptosis of hepatocellular carcinoma cells by NF-κB pathway.; Authors - Qiang Li
rat	IF	Int Immunopharmacol; Polydatin alleviates traumatic spinal cord injury by reducing microglial inflammation via regulation of iNOS and NLRP3 inflammasome pathway.; Authors - Runxiao Lv

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