NGFR/TNFRSF16 Polyclonal antibody

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  WB analysis using 32505-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32505-1-AP (NGFR/TNFRSF16 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of rat brain using 32505-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 32505-1-AP (NGFR/TNFRSF16 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

NGFR/TNFRSF16 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 32505-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, ELISA

Synonyms

CD271, Low affinity neurotrophin receptor p75NTR, Low-affinity nerve growth factor receptor, Low-affinity nerve growth factor receptor p75NGFR, Low-affinity nerve growth factor receptor p75NGR

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Tested Applications

• Positive WB detected in: PC-12 cells
• Positive IHC detected in: rat brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

32505-1-AP targets NGFR/TNFRSF16 in WB, IHC, ELISA applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NGFR/TNFRSF16 fusion protein Eg2651
• Full Name: nerve growth factor receptor (TNFR superfamily, member 16)
• Calculated Molecular Weight: 46 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: NM_033217.3
• Gene Symbol: Ngfr
• Gene ID (NCBI): 18053
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q9Z0W1-1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NGFR, also named as TNFRSF16, Gp80-LNGFR, p75 ICD and CD271, is a low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. It mediates cell survival as well as cell death of neural cells. The antibody is specific to NGFR.