NMDAR2A/GRIN2A Polyclonal antibody

NMDAR2A/GRIN2A Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 28525-1-AP

Synonyms

GRIN2A, hNR2A, NMDAR2A/GRIN2A, NR2A



Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IP detected inrat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

28525-1-AP targets NMDAR2A/GRIN2A in WB, IP, IHC, IF, ELISA applications and shows reactivity with mouse, rat samples.

Tested Reactivity mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NMDAR2A/GRIN2A fusion protein Ag29101
Full Name glutamate receptor, ionotropic, N-methyl D-aspartate 2A
Calculated molecular weight 165 kDa
Observed molecular weight160-180 kDa
GenBank accession numberNM_000833
Gene symbol GRIN2A
Gene ID (NCBI) 2903
RRIDAB_2881163
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GRIN2A (glutamate ionotropic receptor NMDA type subunit 2A), also known as NMDAR2A. And its molecular weight is 165 kDa. GRIN2A is located in cell projection, dendritic spine, cell membrane, synapse, postsynaptic cell membrae, cytolamic vesicle membrane, which is expressed in many tissues, highest expression in brain and heart. This gene encodes a member of the glutamate-gated ion channel protein family. The encoded protein is an N-methyl-D-aspartate (NMDA) receptor subunit. NMDA receptors are both ligand-gated and voltage-dependent, and are involved in long-term potentiation, an activity-dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. These receptors are permeable to calcium ions, and activation results in a calcium influx into post-synaptic cells, which results in the activation of several signaling cascades. Disruption of this gene is associated with focal epilepsy and speech disorder with or without cognitive disability. Alternative splicing results in multiple transcript variants.

Protocols

Product Specific Protocols
WB protocol for NMDAR2A/GRIN2A antibody 28525-1-APDownload protocol
IHC protocol for NMDAR2A/GRIN2A antibody 28525-1-APDownload protocol
IP protocol for NMDAR2A/GRIN2A antibody 28525-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Adv Sci (Weinh)

Single-Nucleus RNA Sequencing Reveals that Decorin Expression in the Amygdala Regulates Perineuronal Nets Expression and Fear Conditioning Response after Traumatic Brain Injury.

Authors - Yingwu Shi
mouseWB,IF

Transl Psychiatry

Reduction of p11 in dorsal raphe nucleus serotonergic neurons mediates depression-like behaviors

Authors - Wei Li
ratWB

J Affect Disord

Dysregulation of striatal dopamine D2/D3 receptor-mediated by hypocretin induces depressive behaviors in rats

Authors - Bing Chen
humanWB

Andrology

Catch-up fat in male adults induces low testosterone and consequently promotes metabolic abnormalities and cognitive impairment.

Authors - XiaoHuan Liu
ratWB

Psychiatry Res

The role of reelin in the pathological mechanism of depression from clinical to rodents

Authors - Kangyu Jin
ratWB

Front Pharmacol

Characterization of three naturally occurring lignans, sesamol, sesamolin, and sesamin, as potent inhibitors of human cytochrome P450 46A1: Implications for treating excitatory neurotoxicity

Authors - Jie Du