NMDAR2A/GRIN2A Polyclonal antibody

NMDAR2A/GRIN2A Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 28525-1-AP

Synonyms

GRIN2A, hNR2A, NMDAR2A/GRIN2A, NR2A

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Validation Data Gallery

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  WB analysis using 28525-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28525-1-AP (NMDAR2A/GRIN2A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of rat brain using 28525-1-AP

  IP result of anti-NMDAR2A/GRIN2A (IP:28525-1-AP, 4ug; Detection:28525-1-AP 1:4000) with rat brain tissue lysate 1120 ug.

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  IHC staining of mouse brain using 28525-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28525-1-AP (NMDAR2A/GRIN2A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 28525-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28525-1-AP (NMDAR2A/GRIN2A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IP detected in: rat brain tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 6 publications below
• IF: See 1 publications below

Product Information

28525-1-AP targets NMDAR2A/GRIN2A in WB, IP, IHC, IF, ELISA applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NMDAR2A/GRIN2A fusion protein Ag29101
• Full Name: glutamate receptor, ionotropic, N-methyl D-aspartate 2A
• Calculated Molecular Weight: 165 kDa
• Observed Molecular Weight: 160-180 kDa
• GenBank Accession Number: NM_000833
• Gene Symbol: GRIN2A
• Gene ID (NCBI): 2903
• RRID: AB_2881163
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GRIN2A (glutamate ionotropic receptor NMDA type subunit 2A), also known as NMDAR2A. And its molecular weight is 165 kDa. GRIN2A is located in cell projection, dendritic spine, cell membrane, synapse, postsynaptic cell membrae, cytolamic vesicle membrane, which is expressed in many tissues, highest expression in brain and heart. This gene encodes a member of the glutamate-gated ion channel protein family. The encoded protein is an N-methyl-D-aspartate (NMDA) receptor subunit. NMDA receptors are both ligand-gated and voltage-dependent, and are involved in long-term potentiation, an activity-dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. These receptors are permeable to calcium ions, and activation results in a calcium influx into post-synaptic cells, which results in the activation of several signaling cascades. Disruption of this gene is associated with focal epilepsy and speech disorder with or without cognitive disability. Alternative splicing results in multiple transcript variants.

Publications

Species	Application	Title
mouse	WB	Adv Sci (Weinh); Single-Nucleus RNA Sequencing Reveals that Decorin Expression in the Amygdala Regulates Perineuronal Nets Expression and Fear Conditioning Response after Traumatic Brain Injury.; Authors - Yingwu Shi
mouse	WB,IF	Transl Psychiatry; Reduction of p11 in dorsal raphe nucleus serotonergic neurons mediates depression-like behaviors; Authors - Wei Li
rat	WB	J Affect Disord; Dysregulation of striatal dopamine D2/D3 receptor-mediated by hypocretin induces depressive behaviors in rats; Authors - Bing Chen
human	WB	Andrology; Catch-up fat in male adults induces low testosterone and consequently promotes metabolic abnormalities and cognitive impairment.; Authors - XiaoHuan Liu
rat	WB	Psychiatry Res; The role of reelin in the pathological mechanism of depression from clinical to rodents; Authors - Kangyu Jin
rat	WB	Front Pharmacol; Characterization of three naturally occurring lignans, sesamol, sesamolin, and sesamin, as potent inhibitors of human cytochrome P450 46A1: Implications for treating excitatory neurotoxicity; Authors - Jie Du