Tested Applications
| Positive WB detected in | mouse brain tissue, rat brain tissue |
| Positive IP detected in | rat brain tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
28525-1-AP targets NMDAR2A/GRIN2A in WB, IHC, IF, IP, ELISA applications and shows reactivity with mouse, rat samples.
| Tested Reactivity | mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag29101 Product name: Recombinant human NMDAR2A/GRIN2A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 933-1127 aa of NM_000833 Sequence: DMVSDKGNLMYSDNRSFQGKESIFGDNMNELQTFVANRQKDNLNNYVFQGQHPLTLNESNPNTVEVAVSTESKANSRPRQLWKKSVDSIRQDSLSQNPVSQRDEATAENRTHSLKSPRYLPEEMAHSDISETSNRATCHREPDNSKNHKTKDNFKRSVASKYPKDCSEVERTYLKTKSSSPRDKIYTIDGEKEPG Predict reactive species |
| Full Name | glutamate receptor, ionotropic, N-methyl D-aspartate 2A |
| Calculated Molecular Weight | 165 kDa |
| Observed Molecular Weight | 160-180 kDa |
| GenBank Accession Number | NM_000833 |
| Gene Symbol | GRIN2A |
| Gene ID (NCBI) | 2903 |
| RRID | AB_2881163 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q12879 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
GRIN2A (glutamate ionotropic receptor NMDA type subunit 2A), also known as NMDAR2A. And its molecular weight is 165 kDa. GRIN2A is located in cell projection, dendritic spine, cell membrane, synapse, postsynaptic cell membrae, cytolamic vesicle membrane, which is expressed in many tissues, highest expression in brain and heart. This gene encodes a member of the glutamate-gated ion channel protein family. The encoded protein is an N-methyl-D-aspartate (NMDA) receptor subunit. NMDA receptors are both ligand-gated and voltage-dependent, and are involved in long-term potentiation, an activity-dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. These receptors are permeable to calcium ions, and activation results in a calcium influx into post-synaptic cells, which results in the activation of several signaling cascades. Disruption of this gene is associated with focal epilepsy and speech disorder with or without cognitive disability. Alternative splicing results in multiple transcript variants.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for NMDAR2A/GRIN2A antibody 28525-1-AP | Download protocol |
| IP protocol for NMDAR2A/GRIN2A antibody 28525-1-AP | Download protocol |
| WB protocol for NMDAR2A/GRIN2A antibody 28525-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The NMDAR2A/GRIN2A Polyclonal antibody (Cat# 28525-1-AP) has 26 citations across journals including Molecular Neurodegeneration, Experimental & Molecular Medicine, Theranostics, Advanced Science, Molecular Psychiatry, Translational Psychiatry, Journal of Medicinal Chemistry, Journal of Agricultural and Food Chemistry, Cells, Journal of Affective Disorders, International Immunopharmacology, Neurochemistry International, Frontiers in Pharmacology, Bioorganic Chemistry, Psychiatry Research, Progress in Neurobiology, Frontiers in Cellular Neuroscience, Journal of Neuroscience, Andrology, Translational Cancer Research, CNS & Neurological Sciences – Therapeutics, and Journal of Hazardous Materials. Research fields center on neuroscience: Alzheimer's disease, depression, epilepsy, stroke, synaptic plasticity, and NMDA receptor signaling.
| Species | Application | Title |
|---|---|---|
Mol Neurodegener Increased expression of mesencephalic astrocyte-derived neurotrophic factor (MANF) contributes to synapse loss in Alzheimer's disease | ||
Exp Mol Med HMGCS2-dependent β-OHB/H3K9bhb ameliorates synaptic plasticity and cognition in Alzheimer's disease. | ||
Theranostics Alisol A ameliorates vascular cognitive impairment via AMPK/NAMPT/SIRT1-mediated regulation of cholesterol and autophagy. | ||
Adv Sci (Weinh) Single-Nucleus RNA Sequencing Reveals that Decorin Expression in the Amygdala Regulates Perineuronal Nets Expression and Fear Conditioning Response after Traumatic Brain Injury. | ||
Mol Psychiatry Downregulation of integrin α3 in ADHD mirrored in mutant mouse model by dopamine-dependent hippocampal AMPAR expression. | ||
Mol Psychiatry Effects of esketamine on electrophysiology and metabolic reprogramming in brain organoids: insights into antidepressant mechanisms |
