NONO Polyclonal antibody

• Featured Product
• KD/KO Validated

NONO Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, IP, CoIP, ChIP, RIP, ELISA

Conjugate

Unconjugated

Publications

43

Reviews (3)

Cat no : 11058-1-AP

Synonyms

P54, NRB54, Non-POU domain-containing octamer-binding protein, NonO protein, 55 kDa nuclear protein

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Validation Data Gallery

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  WB analysis using 11058-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11058-1-AP (NONO antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 11058-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11058-1-AP (NONO antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 11058-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 11058-1-AP (NONO antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 11058-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 11058-1-AP (NONO Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 11058-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11058-1-AP (NONO antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human pancreas using 11058-1-AP

  human pancreas tissue were subjected to SDS PAGE followed by western blot with 11058-1-AP (NONO antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human skeletal muscle using 11058-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11058-1-AP (NONO antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 11058-1-AP

  IP result of anti-NONO (IP:11058-1-AP, 4ug; Detection:11058-1-AP 1:1000) with HEK-293 cells lysate 2800ug.

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  IHC staining of human colon cancer using 11058-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11058-1-AP (NONO antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 11058-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11058-1-AP (NONO antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HEK-293 using 11058-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using NONO antibody (11058-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HEK-293 using 11058-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using NONO antibody (11058-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HCT 116 using 11058-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HCT 116 cells using NONO antibody (11058-1-AP) at dilution of 1:400 and CoraLite®647-conjugated AffiniPure F(ab, CL555-Phalloidin (orange), CoraLite® Plus 488 Alpha Tubulin antibody (CL488-66031, Clone: 1E4C11, green).

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Tested Applications

• Positive WB detected in: PC-3 cells, human pancreas tissue, human skeletal muscle tissue, MCF-7 cells, mouse pancreas tissue, HEK-293 cells, PC-12 cells, NIH/3T3 cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human colon cancer tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HEK-293 cells, HCT 116 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 8 publications below
• WB: See 33 publications below
• IHC: See 4 publications below
• IF: See 12 publications below
• IP: See 5 publications below
• CoIP: See 1 publications below
• ChIP: See 1 publications below
• RIP: See 3 publications below

Product Information

11058-1-AP targets NONO in WB, IHC, IF/ICC, IP, CoIP, ChIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, zebrafish, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NONO fusion protein Ag1512
• Full Name: non-POU domain containing, octamer-binding
• Calculated Molecular Weight: 54 kDa
• Observed Molecular Weight: 54 kDa
• GenBank Accession Number: BC010049
• Gene Symbol: NONO
• Gene ID (NCBI): 4841
• RRID: AB_2152167
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Non-pou domain-containing octamer-binding protein(NONO), other named p54nrb, belongs to DBHS family, whose protein are predominately nuclear and involves in transcriptional and posttranscriptional gene regulatory functions as well as DNA repair. This family has roles in a wide range of biological processes, such as the regulation of circadian rhythm, carcinogenesis and progression of cancer. NONO forms heterodimer with SFPQ, and has been implicated in nuclear retention of A- to I-edited RNA as paraspeckle components, pre-mRAN 3'-end formation, cAMP cycling and transcriptional activation.

Publications

Species	Application	Title
human	WB	Cell Res; Comparison of viral RNA-host protein interactomes across pathogenic RNA viruses informs rapid antiviral drug discovery for SARS-CoV-2.; Authors - Shaojun Zhang
human	WB	Nat Commun; K235 acetylation couples with PSPC1 to regulate the m6A demethylation activity of ALKBH5 and tumorigenesis; Authors - Xiao-Lan Zhang
human	IF	Mol Cell; Condensates induced by transcription inhibition localize active chromatin to nucleoli.; Authors - Takaaki Yasuhara
human	WB,RIP	Adv Sci (Weinh); The Conserved LncRNA DIO3OS Restricts Hepatocellular Carcinoma Stemness by Interfering with NONO-Mediated Nuclear Export of ZEB1 mRNA; Authors - Ya-Rui Hou ; KD Validated
human	IF	Hepatology; Splicing Regulator p54nrb /NONO Enhances Carcinogenesis Through Oncogenic Isoform Switch of BIN1 in Hepatocellular Carcinoma.; Authors - Zhixiang Hu
human	WB,IHC,IF	Sci Adv; Interfering MSN-NONO complex-activated CREB signaling serves as a therapeutic strategy for triple-negative breast cancer.; Authors - Yuanyuan Qin ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Roy (Verified Customer) (06-28-2024)

Perfect antibody to detect NONO by WB (Overnight incubation at 4°C, 1/1000 dilution)

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HeLa and Jurkat cells

FH

Xingyuan (Verified Customer) (07-17-2023)

The band quality was decent.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: 293T

NONO Antibody Western Blot validation (1:2000 dilution) in 293T (Cat no:11058-1-AP)

FH

Tatyana (Verified Customer) (05-14-2023)

ICC with antibody diluted in 5% goat serum in PBST, with 2 h incubation at RT. Good nuclear staining

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HeLa

NONO Antibody Immunofluorescence validation (1:1000 dilution) in HeLa (Cat no:11058-1-AP)

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