NONO Monoclonal antibody

NONO Monoclonal Antibody for WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

2A2B10

Cat no : 66361-1-Ig

Synonyms

55 kDa nuclear protein, NONO, NonO protein, NRB54, P54, p54(nrb), P54NRB

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Validation Data Gallery

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  WB analysis of C2C12 using 66361-1-Ig

  C2C12 cell were subjected to SDS PAGE followed by western blot with 66361-1-Ig (NONO Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of ROS1728 using 66361-1-Ig

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 66361-1-Ig (NONO Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 66361-1-Ig

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66361-1-Ig (NONO Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: C2C12 cell, mouse skeletal muscle tissue, ROS1728 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

66361-1-Ig targets NONO in WB, ELISA applications and shows reactivity with human, rat, mouse samples.

• Tested Reactivity: human, rat, mouse
• Cited Reactivity: mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: NONO fusion protein Ag17609
• Full Name: non-POU domain containing, octamer-binding
• Calculated Molecular Weight: 54 kDa
• Observed Molecular Weight: 54 kDa
• GenBank Accession Number: BC010049
• Gene Symbol: NONO
• Gene ID (NCBI): 4841
• RRID: AB_2881741
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Non-pou domain-containing octamer-binding protein(NONO), other named p54nrb, belongs to DBHS family, whose protein are predominately nuclear and involves in transcriptional and posttranscriptional gene regulatory functions as well as DNA repair. This family has roles in a wide range of biological processes, such as the regulation of circadian rhythm, carcinogenesis and progression of cancer. NONO forms heterodimer with SFPQ, and has been implicated in nuclear retention of A- to I-edited RNA as paraspeckle components, pre-mRAN 3'-end formation, cAMP cycling and transcriptional activation.

Publications

Species	Application	Title
mouse	WB	Nat Commun; Circular RNA circTmem241 drives group III innate lymphoid cell differentiation via initiation of Elk3 transcription; Authors - Nian Liu