NOP16 Polyclonal antibody

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  WB analysis using 31969-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31969-1-AP (NOP16 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

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  IF Staining of A431 using 31969-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using NOP16 antibody (31969-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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  IF Staining of MCF-7 using 31969-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using NOP16 antibody (31969-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

NOP16 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 31969-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

NOP16 Nucleolar Protein, Hypothetical Protein HSPC111, HSPC185, HSPC111, HBV pre-S2 trans-regulated protein 3

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Tested Applications

• Positive WB detected in: A431 cells, Hela cells, K-562 cells, MCF-7 cells, Raji cells
• Positive IF/ICC detected in: A431 cells, MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31969-1-AP targets NOP16 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NOP16 fusion protein Ag36714
• Full Name: NOP16 nucleolar protein homolog (yeast)
• Observed Molecular Weight: 23 kDa
• GenBank Accession Number: BC040106
• Gene Symbol: NOP16
• Gene ID (NCBI): 51491
• RRID: AB_3670160
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q9Y3C1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NOP16, also known as HSPC111, is found in the intracellular protein complex. It is a ribosomal protein that is transcriptionally regulated by the oncogene c-Myc. NOP16 may be involved in the completion of ribosome synthesis, therefore, reduced NOP16 expression in cancer cells may block many biological processes. Studies indicate that NOP16 is highly expressed in more than 20 types of cancers, including colorectal, breast, and hepatocellular carcinoma, and is an important target for clinical treatment of related diseases.