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NOTCH1 Polyclonal antibody
NOTCH1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Cat no : 20687-1-AP
Validation Data Gallery
|Positive WB detected in
|HEK-293 cells, A2780 cells, HeLa cells, HepG2 cells, Jurkat cells
|Positive IP detected in
|Positive IHC detected in
|human breast cancer tissue, human ovary tumor tissue, mouse brain tissue, human lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|Western Blot (WB)
|WB : 1:500-1:1000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:50-1:500
|IF : 1:200-1:800
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
20687-1-AP targets NOTCH1 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse samples.
|human, mouse, rat, mink, zebrafish, pig
|Host / Isotype
|Rabbit / IgG
|Notch homolog 1, translocation-associated (Drosophila)
|Calculated molecular weight
|Observed molecular weight
|273-300 kDa, 120 kDa
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
NOTCH1, also named as TAN1, belongs to the NOTCH family. NOTCH1 functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBP-J kappa and activates genes of the enhancer of split locus. NOTCH1 affects the implementation of differentiation, proliferation and apoptotic programs. It may be important for normal lymphocyte function. In altered form, may contribute to transformation or progression in some T-cell neoplasms. NOTCH1 is involved in the maturation of both CD4+ and CD8+ cells in the thymus. May be important for follicular differentiation and possibly cell fate selection within the follicle. During cerebellar development, may function as a receptor for neuronal DNER and may be involved in the differentiation of Bergmann glia. Defects in NOTCH1 are a cause of bicuspid aortic valve (BAV).
Notch is synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase (S1 cleavage) in the trans-golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase (S3 cleavage) to release the intracellular domain (NICD) from the membrane. The antibody is specific to NOTCH1. It can recognize the full length NOTCH1(270 kDa) and cleaved NOTCH1 form (120 kDa).
|Product Specific Protocols
|WB protocol for NOTCH1 antibody 20687-1-AP
|IHC protocol for NOTCH1 antibody 20687-1-AP
|IF protocol for NOTCH1 antibody 20687-1-AP
|IP protocol for NOTCH1 antibody 20687-1-AP
|Click here to view our Standard Protocols
Divergent transcriptional regulation of astrocyte reactivity across disorders.
Mitochondrial Dynamics Is Critical for the Full Pluripotency and Embryonic Developmental Potential of Pluripotent Stem Cells.
Medial prefrontal cortex Notch1 signalling mediates methamphetamine-induced psychosis via Hes1-dependent suppression of GABAB1 receptor expression.
Immunological profiles of human oligodendrogliomas define two distinct molecular subtypes
Transcriptional control of brain tumor stem cells by a carbohydrate binding protein
Int J Cancer
Modification of α2,6-sialylation mediates the invasiveness and tumorigenicity of non-small cell lung cancer cells in vitro and in vivo via Notch1/Hes1/MMPs pathway.