NOTCH1 Polyclonal antibody

• Featured Product
• KD/KO Validated

NOTCH1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (3)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

84

Cat no : 20687-1-AP

Synonyms

Translocation-associated notch protein TAN-1, TAN1, Notch 1, hN1

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Validation Data Gallery

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  WB analysis using 20687-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20687-1-AP (NOTCH1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 20687-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20687-1-AP (NOTCH1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 20687-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20687-1-AP (NOTCH1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 20687-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20687-1-AP (NOTCH1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 20687-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20687-1-AP (NOTCH1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 20687-1-AP

  IP result of anti-NOTCH1 (IP:20687-1-AP, 4ug; Detection:20687-1-AP 1:600) with HEK-293 cells lysate 1480 ug.

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  IP experiment of HepG2 using 20687-1-AP

  IP result of anti-NOTCH1 (IP:20687-1-AP, 4ug; Detection:20687-1-AP 1:500) with HepG2 cells lysate 1360 ug.

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  IHC staining of human breast cancer using 20687-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20687-1-AP (NOTCH1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 20687-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20687-1-AP (NOTCH1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 20687-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 20687-1-AP (NOTCH1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 20687-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 20687-1-AP (NOTCH1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 20687-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 20687-1-AP (NOTCH1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 20687-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 20687-1-AP (NOTCH1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 20687-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 20687-1-AP (NOTCH1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 20687-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NOTCH1 antibody (20687-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: HEK-293 cells, A2780 cells, HeLa cells, HepG2 cells, Jurkat cells
• Positive IP detected in: HEK-293 cells, HepG2 cells
• Positive IHC detected in: human breast cancer tissue, human ovary tumor tissue, human lymphoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 70 publications below
• IHC: See 12 publications below
• IF: See 10 publications below
• IP: See 1 publications below
• FC: See 1 publications below
• CoIP: See 5 publications below

Product Information

20687-1-AP targets NOTCH1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, pig, zebrafish, mink
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: Notch homolog 1, translocation-associated (Drosophila)
• Calculated Molecular Weight: 273 kDa
• Observed Molecular Weight: 273-300 kDa, 120 kDa
• GenBank Accession Number: NM_017617
• Gene Symbol: NOTCH1
• Gene ID (NCBI): 4851
• RRID: AB_10700012
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NOTCH1, also named as TAN1, belongs to the NOTCH family. NOTCH1 functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBP-J kappa and activates genes of the enhancer of split locus. NOTCH1 affects the implementation of differentiation, proliferation and apoptotic programs. It may be important for normal lymphocyte function. In altered form, may contribute to transformation or progression in some T-cell neoplasms. NOTCH1 is involved in the maturation of both CD4+ and CD8+ cells in the thymus. May be important for follicular differentiation and possibly cell fate selection within the follicle. During cerebellar development, may function as a receptor for neuronal DNER and may be involved in the differentiation of Bergmann glia. Defects in NOTCH1 are a cause of bicuspid aortic valve (BAV).

Notch is synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase (S1 cleavage) in the trans-golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase (S3 cleavage) to release the intracellular domain (NICD) from the membrane. The antibody is specific to NOTCH1. It can recognize the full length NOTCH1(270 kDa) and cleaved NOTCH1 form (120 kDa).

Publications

Species	Application	Title
	IHC	Nature; Divergent transcriptional regulation of astrocyte reactivity across disorders.; Authors - Joshua E Burda
mouse	WB	Cell Metab; Mitochondrial Dynamics Is Critical for the Full Pluripotency and Embryonic Developmental Potential of Pluripotent Stem Cells.; Authors - Xiuying Zhong
	IF	Mol Psychiatry; Medial prefrontal cortex Notch1 signalling mediates methamphetamine-induced psychosis via Hes1-dependent suppression of GABAB1 receptor expression.; Authors - Tong Ni
human	IHC	EBioMedicine; Immunological profiles of human oligodendrogliomas define two distinct molecular subtypes; Authors - Fan Wu
human	WB	Cell Rep; Transcriptional control of brain tumor stem cells by a carbohydrate binding protein; Authors - Ahmad Sharanek
human,mouse	WB,IHC	Int J Cancer; Modification of α2,6-sialylation mediates the invasiveness and tumorigenicity of non-small cell lung cancer cells in vitro and in vivo via Notch1/Hes1/MMPs pathway.; Authors - Qingmin Yuan

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