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- KD/KO Validated
NOTCH1 Polyclonal antibody
NOTCH1 Polyclonal Antibody for IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
human and More (5)
WB, IHC, IF, FC, CoIP, ELISA
Cat no : 20687-1-AP
Validation Data Gallery
|Positive WB detected in||HEK-293 cells, A2780 cells, HeLa cells, HepG2 cells, Jurkat cells|
|Positive IHC detected in||human breast cancer tissue, human ovary tumor tissue, mouse brain tissue, human lymphoma tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
|KD/KO||See 1 publications below|
|WB||See 55 publications below|
|IHC||See 10 publications below|
|IF||See 8 publications below|
|FC||See 1 publications below|
|CoIP||See 1 publications below|
20687-1-AP targets NOTCH1 in WB, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human samples.
|Cited Reactivity||human, mouse, rat, mink, pig, zebrafish|
|Host / Isotype||Rabbit / IgG|
|Full Name||Notch homolog 1, translocation-associated (Drosophila)|
|Calculated molecular weight||273 kDa|
|Observed molecular weight||273-300 kDa, 120 kDa|
|GenBank accession number||NM_017617|
|Gene ID (NCBI)||4851|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
NOTCH1, also named as TAN1, belongs to the NOTCH family. NOTCH1 functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBP-J kappa and activates genes of the enhancer of split locus. NOTCH1 affects the implementation of differentiation, proliferation and apoptotic programs. It may be important for normal lymphocyte function. In altered form, may contribute to transformation or progression in some T-cell neoplasms. NOTCH1 is involved in the maturation of both CD4+ and CD8+ cells in the thymus. May be important for follicular differentiation and possibly cell fate selection within the follicle. During cerebellar development, may function as a receptor for neuronal DNER and may be involved in the differentiation of Bergmann glia. Defects in NOTCH1 are a cause of bicuspid aortic valve (BAV).
Notch is synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase (S1 cleavage) in the trans-golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase (S3 cleavage) to release the intracellular domain (NICD) from the membrane. The antibody is specific to NOTCH1. It can recognize the full length NOTCH1(270 kDa) and cleaved NOTCH1 form (120 kDa).
|Product Specific Protocols|
|WB protocol for NOTCH1 antibody 20687-1-AP||Download protocol|
|IHC protocol for NOTCH1 antibody 20687-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Divergent transcriptional regulation of astrocyte reactivity across disorders.
Medial prefrontal cortex Notch1 signalling mediates methamphetamine-induced psychosis via Hes1-dependent suppression of GABAB1 receptor expression.
Immunological profiles of human oligodendrogliomas define two distinct molecular subtypes
Transcriptional control of brain tumor stem cells by a carbohydrate binding protein
Int J Cancer
Modification of α2,6-sialylation mediates the invasiveness and tumorigenicity of non-small cell lung cancer cells in vitro and in vivo via Notch1/Hes1/MMPs pathway.
Vasorin-containing small extracellular vesicles retard intervertebral disc degeneration utilizing an injectable thermoresponsive delivery system