NPTX2 Polyclonal antibody

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  WB analysis using 10889-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10889-1-AP (NPTX2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 10889-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 10889-1-AP (NPTX2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 10889-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 10889-1-AP (NPTX2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 10889-1-AP

  IP result of anti-NPTX2 (IP:10889-1-AP, 4ug; Detection:10889-1-AP 1:600) with mouse brain tissue lysate 4000ug.

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  IHC staining of human brain using 10889-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10889-1-AP (NPTX2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human brain using 10889-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10889-1-AP (NPTX2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 10889-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10889-1-AP (NPTX2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human pancreas cancer using 10889-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10889-1-AP (NPTX2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 10889-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10889-1-AP (NPTX2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of SH-SY5Y using 10889-1-AP

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using NPTX2 antibody (10889-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

• Featured Product
• KD/KO Validated

NPTX2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 10889-1-AP

Publications(20)

Reviews (2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

NP-II, NP2, Neuronal pentraxin-2, Neuronal pentraxin II

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Tested Applications

• Positive WB detected in: mouse brain tissue, SH-SY5Y cells, PC-3 cells, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human brain tissue, human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 11 publications below
• IHC: See 6 publications below
• IF: See 9 publications below
• FC: See 1 publications below
• CoIP: See 1 publications below

Product Information

10889-1-AP targets NPTX2 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1326

  Product name: Recombinant human NPTX2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-350 aa of BC009924

  Sequence: MLALLAASVALAVAAGAQDSPAPGSRFVCTALPPEAVHAGCPLPAMPMQGGAQSPEEELRAAVLQLRETVVQQKETLGAQREAIRELTGKLARCEGLAGGKARGAGATGKDTMGDLPRDPGHVVEQLSRSLQTLKDRLESLEHQLRANVSNAGLPGDFREVLQQRLGELERQLLRKVAELEDEKSLLHNETSAHRQKTESTLNALLQRVTELERGNSAFKSPDAFKVSLPLRTNYLYGKIKKTLPELYAFTICLWLRSSASPGIGTPFSYAVPGQANEIVLIEWGNNPIELLINDKVAQLPLFVSDGKWHHICVTWTTRDGMWEAFQDGEKLGTGENLAPWHPIKPGGVL

• Full Name: neuronal pentraxin II
• Calculated Molecular Weight: 47 kDa
• Observed Molecular Weight: 47-50 kDa
• GenBank Accession Number: BC009924
• Gene Symbol: NPTX2
• Gene ID (NCBI): 4885
• RRID: AB_2153875
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P47972
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Neuronal pentraxins constitute a family of proteins that are homologous to C-reactive protein (CRP) and serum amyloid P component (SAP), including NPTX1, NPTX2, and the neuronal pentraxin receptor (NPTXR). NPTX2, also known as NARP (neuronal activity-regulated pentaxin), is a secreted protein involved in excitatory synapse formation. It also plays a role in the clustering of alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)-type glutamate receptors at established synapses, resulting in non-apoptotic cell death of dopaminergic nerve cells. NPTX2 is highly up-regulated in Parkinson's disease (PD), suggesting it may be involved in the pathology of PD. (PMID: 12895424; 17987278; 10748068)

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IHC,IF	J Invest Dermatol; NPTX2 Promotes EMT in Cutaneous Squamous Cell Carcinoma via Mettl3-Mediated m6A Methylation of Snail; Authors - Tong Kang
mouse	IHC,IF	Neuropsychopharmacology; NPTX2 is a key component in the regulation of anxiety.; Authors - Simon Chang ; KO Validated
mouse	IF	Front Immunol; Identification of Neuronal Pentraxins as Synaptic Binding Partners of C1q and the Involvement of NP1 in Synaptic Pruning in Adult Mice.; Authors - Réka Á Kovács
human	IF	J Neurochem; Quantitative proteomic analysis of the frontal cortex in Alzheimer's disease.; Authors - Gajanan Sathe
human	WB,IHC	J Cancer; Promoter Methylation-Mediated NPTX2 Silencing Promotes Tumor Growth in Human Prostate Cancer.; Authors - Yixi Su
human	WB,IHC	Mol Biol Rep; The neuronal pentraxin II gene (NPTX2) inhibit proliferation and invasion of pancreatic cancer cells in vitro.; Authors - Zhang Ling L

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Xin (Verified Customer) (10-10-2022)

Very good with WB to detect endogenous NPTX2 with a clean and clear band with the right MW.

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: mouse brain lysate

NPTX2 Antibody Western Blot validation (1:3000 dilution) in mouse brain lysate (Cat no:10889-1-AP)

FH

Jerome (Verified Customer) (05-07-2019)

This protein should be in the secretory pathway but instead the antibody stains the nucleus

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 100
• Cell Tissue Type: Hela Cells

NPTX2 Antibody Immunofluorescence, validation (100 dilution) in Hela Cells (Cat no:10889-1-AP)