Tested Applications
| Positive WB detected in | mouse brain tissue, SH-SY5Y cells, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human brain tissue, human pancreas cancer tissue, rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | SH-SY5Y cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10889-1-AP targets NPTX2 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1326 Product name: Recombinant human NPTX2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-354 aa of BC009924 Sequence: MLALLAASVALAVAAGAQDSPAPGSRFVCTALPPEAVHAGCPLPAMPMQGGAQSPEEELRAAVLQLRETVVQQKETLGAQREAIRELTGKLARCEGLAGGKARGAGATGKDTMGDLPRDPGHVVEQLSRSLQTLKDRLESLEHQLRANVSNAGLPGDFREVLQQRLGELERQLLRKVAELEDEKSLLHNETSAHRQKTESTLNALLQRVTELERGNSAFKSPDAFKVSLPLRTNYLYGKIKKTLPELYAFTICLWLRSSASPGIGTPFSYAVPGQANEIVLIEWGNNPIELLINDKVAQLPLFVSDGKWHHICVTWTTRDGMWEAFQDGEKLGTGENLAPWHPIKPGGVLILGQ Predict reactive species |
| Full Name | neuronal pentraxin II |
| Calculated Molecular Weight | 47 kDa |
| Observed Molecular Weight | 47-50 kDa, 55-60 kDa |
| GenBank Accession Number | BC009924 |
| Gene Symbol | NPTX2 |
| Gene ID (NCBI) | 4885 |
| RRID | AB_2153875 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P47972 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Neuronal pentraxins constitute a family of proteins that are homologous to C-reactive protein (CRP) and serum amyloid P component (SAP), including NPTX1, NPTX2, and the neuronal pentraxin receptor (NPTXR). NPTX2, also known as NARP (neuronal activity-regulated pentaxin), is a secreted protein involved in excitatory synapse formation. It also plays a role in the clustering of alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)-type glutamate receptors at established synapses, resulting in non-apoptotic cell death of dopaminergic nerve cells. NPTX2 is highly up-regulated in Parkinson's disease (PD), suggesting it may be involved in the pathology of PD. (PMID: 12895424; 17987278; 10748068)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for NPTX2 antibody 10889-1-AP | Download protocol |
| IHC protocol for NPTX2 antibody 10889-1-AP | Download protocol |
| IP protocol for NPTX2 antibody 10889-1-AP | Download protocol |
| WB protocol for NPTX2 antibody 10889-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-NPTX2 antibody (Cat# 10889-1-AP) has accumulated 23 citations published in high-impact journals including Nature, Cell Research, Science Translational Medicine, Alzheimer's & Dementia, Cell Death & Disease, Molecular Psychiatry, Cell Reports, Neuropsychopharmacology, and PLoS Biology. Associated research fields encompass neurodegeneration (ALS, FTLD, Alzheimer's disease), synaptic plasticity and memory, cancer biology (colorectal, prostate, pancreatic, renal), neuroimmunology and complement-mediated synaptic pruning, psychiatric disorders (schizophrenia, bipolar disorder, autism spectrum), and neurodevelopmental processes.
| Species | Application | Title |
|---|---|---|
Nature A model of human neural networks reveals NPTX2 pathology in ALS and FTLD
| ||
Cell Res Disturbed engram network caused by NPTX downregulation underlies aging-related contextual fear memory deficits.
| ||
Sci Transl Med The neuronal pentraxin Nptx2 regulates complement activity and restrains microglia-mediated synapse loss in neurodegeneration
| ||
Alzheimers Dement Reduced synaptic proteins and SNARE complexes in Down syndrome with Alzheimer's disease and the Dp16 mouse Down syndrome model: Impact of APP gene dose | ||
Cell Death Dis NPTX2 promotes colorectal cancer growth and liver metastasis by the activation of the canonical Wnt/β-catenin pathway via FZD6. | ||
Mol Psychiatry Loss of schizophrenia risk gene XPO7 disrupts neuronal excitability and network regularity via altered Na(+) channel dynamics in human neurons. |
Reviews
What customers say
The two reviews for this anti-NPTX2 antibody are mixed. One user (5/5) reported excellent Western Blot performance on mouse brain lysate at 1:3000 dilution, noting a clean and clear band at the expected molecular weight. Another user (1/5) was dissatisfied with Immunofluorescence results on HeLa cells at 1:100 dilution, stating that the antibody stained the nucleus despite NPTX2 being a secretory pathway protein. Overall, the antibody appears reliable for WB but may show unexpected localization in IF applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Xin (Verified Customer) (10-10-2022) | Very good with WB to detect endogenous NPTX2 with a clean and clear band with the right MW.
 |
Jerome (Verified Customer) (05-07-2019) | This protein should be in the secretory pathway but instead the antibody stains the nucleus
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