NPTX2 Polyclonal antibody

• Featured Product
• KD/KO Validated

NPTX2 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, CoIP

Conjugate

Unconjugated

Publications

9

Reviews (1)

Cat no : 10889-1-AP

Synonyms

NARP, Neuronal pentraxin 2, neuronal pentraxin II, NPTX2

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Validation Data Gallery

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  WB analysis of mouse brain using 10889-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10889-1-AP (NPTX2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 10889-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 10889-1-AP (NPTX2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 10889-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 10889-1-AP (NPTX2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 10889-1-AP

  IP Result of anti-NPTX2 (IP:10889-1-AP, 4ug; Detection:10889-1-AP 1:600) with mouse brain tissue lysate 4000ug.

• 

  IHC staining of human brain using 10889-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10889-1-AP (NPTX2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 10889-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10889-1-AP (NPTX2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 10889-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10889-1-AP (NPTX2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human pancreas cancer using 10889-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10889-1-AP (NPTX2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 10889-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10889-1-AP (NPTX2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse brain tissue, PC-3 cells, SH-SY5Y cells
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human brain tissue, human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 4 publications below
• IF: See 3 publications below
• CoIP: See 1 publications below

Product Information

10889-1-AP targets NPTX2 in WB, IP, IHC, IF, CoIP applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NPTX2 fusion protein Ag1326
• Full Name: neuronal pentraxin II
• Calculated molecular weight: 47 kDa
• Observed molecular weight: 47 kDa
• GenBank accession number: BC009924
• Gene symbol: NPTX2
• Gene ID (NCBI): 4885
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Neuronal pentraxins constitute a family of proteins that are homologous to C-reactive protein (CRP) and serum amyloid P component (SAP), including NPTX1, NPTX2, and the neuronal pentraxin receptor (NPTXR). NPTX2, also known as NARP (neuronal activity-regulated pentaxin), is secreted protein involved in excitatory synapse formation. It also plays a role in clustering of alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)-type glutamate receptors at established synapses, resulting in non-apoptotic cell death of dopaminergic nerve cells. NPTX2 is highly up-regulated in the Parkinson's disease (PD), suggesting it may be involved in the pathology of PD. (PMID: 12895424; 17987278; 10748068)

Publications

Species	Application	Title
human	CoIP	Cell Death Dis; NPTX2 promotes colorectal cancer growth and liver metastasis by the activation of the canonical Wnt/β-catenin pathway via FZD6.; Authors - Chunjie Xu
mouse	WB	Neuroscience; Profiling the Gene Expression and DNA Methylation in the Mouse Brain after Ischemic Preconditioning.; Authors - Menghua Cai
human	WB	Bioengineered; Silencing circular RNA circ_0054537 and upregulating microRNA-640 suppress malignant progression of renal cell carcinoma via regulating neuronal pentraxin-2 (NPTX2).; Authors - Long Pei
human	IHC	Dig Dis Sci; Neuronal pentraxin II (NPTX2) is frequently down-regulated by promoter hypermethylation in pancreatic cancers.; Authors - Zhang Ling L
human	WB,IHC	Mol Biol Rep; The neuronal pentraxin II gene (NPTX2) inhibit proliferation and invasion of pancreatic cancer cells in vitro.; Authors - Zhang Ling L
human	WB,IHC	J Cancer; Promoter Methylation-Mediated NPTX2 Silencing Promotes Tumor Growth in Human Prostate Cancer.; Authors - Yixi Su

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Jerome (Verified Customer) (05-07-2019)

This protein should be in the secretory pathway but instead the antibody stains the nucleus

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 100
• Cell Tissue Type: Hela Cells

NPTX2 Antibody Immunofluorescence, validation (100 dilution) in Hela Cells (Cat no:10889-1-AP)

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