• Featured Product
  • KD/KO Validated

NPTX2 Polyclonal antibody

NPTX2 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 10889-1-AP

Synonyms

NARP, Neuronal pentraxin 2, neuronal pentraxin II, NPTX2



Tested Applications

Positive WB detected inmouse brain tissue, PC-3 cells, SH-SY5Y cells
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman brain tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10889-1-AP targets NPTX2 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NPTX2 fusion protein Ag1326
Full Name neuronal pentraxin II
Calculated molecular weight 47 kDa
Observed molecular weight 47 kDa
GenBank accession numberBC009924
Gene symbol NPTX2
Gene ID (NCBI) 4885
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Neuronal pentraxins constitute a family of proteins that are homologous to C-reactive protein (CRP) and serum amyloid P component (SAP), including NPTX1, NPTX2, and the neuronal pentraxin receptor (NPTXR). NPTX2, also known as NARP (neuronal activity-regulated pentaxin), is secreted protein involved in excitatory synapse formation. It also plays a role in clustering of alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)-type glutamate receptors at established synapses, resulting in non-apoptotic cell death of dopaminergic nerve cells. NPTX2 is highly up-regulated in the Parkinson's disease (PD), suggesting it may be involved in the pathology of PD. (PMID: 12895424; 17987278; 10748068)

Protocols

Product Specific Protocols
WB protocol for NPTX2 antibody 10889-1-APDownload protocol
IHC protocol for NPTX2 antibody 10889-1-APDownload protocol
IP protocol for NPTX2 antibody 10889-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanCoIP

Cell Death Dis

NPTX2 promotes colorectal cancer growth and liver metastasis by the activation of the canonical Wnt/β-catenin pathway via FZD6.

Authors - Chunjie Xu
mouseWB

Neuroscience

Profiling the Gene Expression and DNA Methylation in the Mouse Brain after Ischemic Preconditioning.

Authors - Menghua Cai
humanWB

Bioengineered

Silencing circular RNA circ_0054537 and upregulating microRNA-640 suppress malignant progression of renal cell carcinoma via regulating neuronal pentraxin-2 (NPTX2).

Authors - Long Pei
humanIHC

Dig Dis Sci

Neuronal pentraxin II (NPTX2) is frequently down-regulated by promoter hypermethylation in pancreatic cancers.

Authors - Zhang Ling L
humanWB,IHC

Mol Biol Rep

The neuronal pentraxin II gene (NPTX2) inhibit proliferation and invasion of pancreatic cancer cells in vitro.

Authors - Zhang Ling L
humanIF

J Neurochem

Quantitative proteomic analysis of the frontal cortex in Alzheimer's disease.

Authors - Gajanan Sathe

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Jerome (Verified Customer) (05-07-2019)

This protein should be in the secretory pathway but instead the antibody stains the nucleus

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 100
  • Cell Tissue Type: Hela Cells
NPTX2 Antibody Immunofluorescence, validation (100 dilution) in Hela Cells (Cat no:10889-1-AP)