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NQO1 Antibody 53 Publications

Rabbit Polyclonal| Catalog number: 11451-1-AP

Featured Product KD/KO validated

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Con: 28 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, FC, ELISA
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Images See all

NQO1 Antibody WB HepG2 cells 11451-1-AP

WB result of NQO1 antibody (11451-1-AP, 1:1000) with si-control and si-NQO1 transfected HepG2 cells.

NQO1 Antibody IF MCF-7 cells 11451-1-AP

Immunofluorescent analysis of MCF-7 cells, using NQO1 antibody 11451-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

NQO1 Antibody WB K-562 cells 11451-1-AP

K-562 cells were subjected to SDS PAGE followed by western blot with 11451-1-AP( NQO1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

NQO1 Antibody WB HepG2 cells 11451-1-AP

HepG2 cells were subjected to SDS PAGE followed by western blot with 11451-1-AP( NQO1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

NQO1 Antibody IP HepG2 cells 11451-1-AP

IP result of anti-NQO1(IP:11451-1-AP, 4ug; Detection:11451-1-AP 1:300) with HepG2 cells lysate 3000 ug

NQO1 Antibody IHC human pancreas cancer tissue 11451-1-AP

Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11451-1-AP( NQO1 Antibody) at dilution of 1:200 (under 10x lens).

NQO1 Antibody IHC human pancreas cancer tissue 11451-1-AP

Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11451-1-AP( NQO1 Antibody) at dilution of 1:200 (under 40x lens).

NQO1 Antibody FC MCF-7 cells 11451-1-AP

1X10^6 MCF-7 cells were stained with 0.2ug NQO1 antibody (11451-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

Species specificity:
human, pig
Positive WB detected in:
K-562 cells, HepG2 cells, MDA-MB-453s cells
Positive IP detected in:
HepG2 cells
Positive IHC detected in:
human pancreas cancer tissue, human colon tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
MCF-7 cells
Positive FC detected in:
MCF-7 cells
Recommended dilution:
WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:150-1:600 for WB
IHC : 1:100-1:500
IF : 1:20-1:200
Sample-dependent, check data in Validation tab

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
NQO1 fusion protein Ag2009
Full name:
NAD(P)H dehydrogenase, quinone 1
Calculated molecular weight:
274aa,31 kDa
Observed molecular weight:
31 kDa
GenBank accession number:
BC007659
Gene ID (NCBI):
1728
Gene symbol
NQO1
Synonyms
Azoreductase, DHQU, DIA4, DT diaphorase, DTD, Menadione reductase, NQO1, Phylloquinone reductase, QR1, Quinone reductase 1

Background

NQO1, also named as DIA4, NMOR1, DTD and QR1, belongs to the NAD(P)H dehydrogenase (quinone) family. This enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis. It is known to be involved in benzene metabolism. In human studies of ozone exposure, polymorphisms in oxidative stress genes (NQO1, GSTM1, GSTP1) modify respiratory symptoms, lung function, biomarkers and risk of asthma. (PMID:18511640; 18848868 ) This antibody recognizes all the three isoforms (26-27 kDa and 31 kDa) of NQO1 and the homo-dimer form (66-70 kDa) of NQO1.


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