NQO1 Polyclonal antibody

• Featured Product
• KD/KO Validated

NQO1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (3)

Applications

WB, IP, IHC, IF, FC

Conjugate

Unconjugated

Publications

149

Cat no : 11451-1-AP

Synonyms

Azoreductase, DHQU, DIA4, DT diaphorase, DTD, Menadione reductase, NQO1, Phylloquinone reductase, QR1, Quinone reductase 1

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Validation Data Gallery

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  WB analysis of HepG2 using 11451-1-AP

  WB result of NQO1 antibody (11451-1-AP, 1:1000) with si-control and si-NQO1 transfected HepG2 cells.

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  IF Staining of MCF-7 using 11451-1-AP

  Immunofluorescent analysis of MCF-7 cells, using NQO1 antibody 11451-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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  IHC staining of human colon cancer using 11451-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11451-1-AP (NQO1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  WB analysis of K-562 using 11451-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 11451-1-AP (NQO1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 11451-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11451-1-AP (NQO1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 11451-1-AP

  IP result of anti-NQO1 (IP:11451-1-AP, 4ug; Detection:11451-1-AP 1:300) with HepG2 cells lysate 3000 ug.

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  FC experiment of MCF-7 using 11451-1-AP

  1X10^6 MCF-7 cells were stained with 0.2ug NQO1 antibody (11451-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Tested Applications

• Positive WB detected in: K-562 cells, HepG2 cells
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: MCF-7 cells
• Positive FC detected in: MCF-7 cells

Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®555 conjugated versions of this antibody.

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:150-1:600 for WB
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 3 publications below
• WB: See 138 publications below
• IHC: See 8 publications below
• IF: See 6 publications below

Product Information

11451-1-AP targets NQO1 in WB, IP, IHC, IF, FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: bovine, human, pig, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NQO1 fusion protein Ag2009
• Full Name: NAD(P)H dehydrogenase, quinone 1
• Calculated molecular weight: 274 aa, 31 kDa
• Observed molecular weight: 31 kDa
• GenBank accession number: BC007659
• Gene symbol: NQO1
• Gene ID (NCBI): 1728
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

NQO1, also named as DIA4, NMOR1, DTD and QR1, belongs to the NAD(P)H dehydrogenase (quinone) family. This enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis. It is known to be involved in benzene metabolism. In human studies of ozone exposure, polymorphisms in oxidative stress genes (NQO1, GSTM1, GSTP1) modify respiratory symptoms, lung function, biomarkers and risk of asthma. (PMID:18511640; 18848868 ) This antibody recognizes all the three isoforms (26-27 kDa and 31 kDa) of NQO1 and the homo-dimer form (66-70 kDa) of NQO1.

Publications

Species	Application	Title
human	WB	Cancer Lett; NAD(P)H: Quinone oxidoreductase 1 overexpression in hepatocellular carcinoma potentiates apoptosis evasion through regulating stabilization of X-linked inhibitor of apoptosis protein.; Authors - Wan-Yu Li ; KD Validated
rat	WB	J Sci Food Agric; l-Methionine activates Nrf2-ARE pathway to induce endogenous antioxidant activity for depressing ROS-derived oxidative stress in growing rats.; Authors - Zhengxuan Wang
rat	WB	Molecules; Neuroprotective Effect of Dioscin on the Aging Brain.; Authors - Yan Qi
mouse	WB	J Agric Food Chem; Comparison of the Hepatoprotective Effects of the Three Main Stilbenes from Mulberry Twigs.; Authors - Ya-Nan Jia
human	WB	ACS Chem Neurosci; Selenepezil, a Selenium-Containing Compound, Exerts Neuroprotective Effect via Modulation of the Keap1-Nrf2-ARE Pathway and Attenuates Aβ-Induced Cognitive Impairment in Vivo.; Authors - Jun Yan
mouse	WB	Biochem Biophys Res Commun; Curcumin upregulates the Nrf2 system by repressing inflammatory signaling-mediated Keap1 expression in insulin-resistant conditions.; Authors - Liwei Ren

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