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- KD/KO Validated
NQO1 Polyclonal antibody
NQO1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (2)
Applications
WB, IP, IHC, IF, FC,ELISA
Conjugate
Unconjugated
102
Cat no : 11451-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | K-562 cells, HepG2 cells, MDA-MB-453s cells |
Positive IP detected in | HepG2 cells |
Positive IHC detected in | human pancreas cancer tissue, human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | MCF-7 cells |
Positive FC detected in | MCF-7 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:150-1:600 for WB |
Immunohistochemistry (IHC) | IHC : 1:100-1:500 |
Immunofluorescence (IF) | IF : 1:20-1:200 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 3 publications below |
WB | See 92 publications below |
IHC | See 7 publications below |
IF | See 6 publications below |
Product Information
11451-1-AP targets NQO1 in WB, IP, IHC, IF, FC,ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Cited Reactivity | bovine, human, swine |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | NQO1 fusion protein Ag2009 |
Full Name | NAD(P)H dehydrogenase, quinone 1 |
Calculated molecular weight | 274 aa, 31 kDa |
Observed molecular weight | 31 kDa |
GenBank accession number | BC007659 |
Gene symbol | NQO1 |
Gene ID (NCBI) | 1728 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
NQO1, also named as DIA4, NMOR1, DTD and QR1, belongs to the NAD(P)H dehydrogenase (quinone) family. This enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis. It is known to be involved in benzene metabolism. In human studies of ozone exposure, polymorphisms in oxidative stress genes (NQO1, GSTM1, GSTP1) modify respiratory symptoms, lung function, biomarkers and risk of asthma. (PMID:18511640; 18848868 ) This antibody recognizes all the three isoforms (26-27 kDa and 31 kDa) of NQO1 and the homo-dimer form (66-70 kDa) of NQO1.
Protocols
Product Specific Protocols | |
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WB protocol for NQO1 antibody 11451-1-AP | Download protocol |
IHC protocol for NQO1 antibody 11451-1-AP | Download protocol |
IF protocol for NQO1 antibody 11451-1-AP | Download protocol |
IP protocol for NQO1 antibody 11451-1-AP | Download protocol |
FC protocol for NQO1 antibody 11451-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cancer Lett NAD(P)H: Quinone oxidoreductase 1 overexpression in hepatocellular carcinoma potentiates apoptosis evasion through regulating stabilization of X-linked inhibitor of apoptosis protein.
| ||
J Sci Food Agric l-Methionine activates Nrf2-ARE pathway to induce endogenous antioxidant activity for depressing ROS-derived oxidative stress in growing rats. | ||
J Agric Food Chem Comparison of the Hepatoprotective Effects of the Three Main Stilbenes from Mulberry Twigs. | ||
ACS Chem Neurosci Selenepezil, a Selenium-Containing Compound, Exerts Neuroprotective Effect via Modulation of the Keap1-Nrf2-ARE Pathway and Attenuates Aβ-Induced Cognitive Impairment in Vivo. | ||
Biochem Biophys Res Commun Curcumin upregulates the Nrf2 system by repressing inflammatory signaling-mediated Keap1 expression in insulin-resistant conditions. |