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  • KD/KO Validated

NQO1 Polyclonal antibody

NQO1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (5)

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11451-1-AP

Synonyms

Azoreductase, DHQU, DIA4, DT diaphorase, DTD, Menadione reductase, NQO1, Phylloquinone reductase, QR1, Quinone reductase 1



Tested Applications

Positive WB detected inHepG2 cells, K-562 cells, LNCaP cells, MCF-7 cells, NIH/3T3 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11451-1-AP targets NQO1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, goat, chicken, rabbit, pig, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NQO1 fusion protein Ag2009
Full Name NAD(P)H dehydrogenase, quinone 1
Calculated Molecular Weight 274 aa, 31 kDa
Observed Molecular Weight 31 kDa
GenBank Accession NumberBC007659
Gene Symbol NQO1
Gene ID (NCBI) 1728
RRIDAB_2298729
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NQO1, also named as DIA4, NMOR1, DTD and QR1, belongs to the NAD(P)H dehydrogenase (quinone) family. This enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis. It is known to be involved in benzene metabolism. In human studies of ozone exposure, polymorphisms in oxidative stress genes (NQO1, GSTM1, GSTP1) modify respiratory symptoms, lung function, biomarkers and risk of asthma. (PMID:18511640; 18848868 ) This antibody recognizes all the three isoforms (26-27 kDa and 31 kDa) of NQO1 and the homo-dimer form (66-70 kDa) of NQO1.

Protocols

Product Specific Protocols
WB protocol for NQO1 antibody 11451-1-APDownload protocol
IHC protocol for NQO1 antibody 11451-1-APDownload protocol
IF protocol for NQO1 antibody 11451-1-APDownload protocol
IP protocol for NQO1 antibody 11451-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nature

The CoQ oxidoreductase FSP1 acts parallel to GPX4 to inhibit ferroptosis.

Authors - Kirill Bersuker
  • KO Validated
mouseWB

Sci Transl Med

Activation of NRF2 ameliorates oxidative stress and cystogenesis in autosomal dominant polycystic kidney disease.

Authors - Yi Lu
humanWB

Mol Cell

Kynurenine importation by SLC7A11 propagates anti-ferroptotic signaling.

Authors - Alessandra Fiore
mouseWB

Nat Commun

Microglia clear neuron-released α-synuclein via selective autophagy and prevent neurodegeneration.

Authors - Insup Choi
humanWB

Nat Commun

Cordycepin prevents radiation ulcer by inhibiting cell senescence via NRF2 and AMPK in rodents.

Authors - Ziwen Wang
pigWB

Microbiome

Antioxidant potential of Pediococcus pentosaceus strains from the sow milk bacterial collection in weaned piglets.

Authors - Leli Wang