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  • KD/KO Validated

NQO1 Antibody

NQO1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (3)

Applications

FC, IF, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat no : 11451-1-AP

Synonyms

Azoreductase, DHQU, DIA4, DT diaphorase, DTD, Menadione reductase, NQO1, Phylloquinone reductase, QR1, Quinone reductase 1



Tested Applications

Positive WB detected inK-562 cells, HepG2 cells, MDA-MB-453s cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman pancreas cancer tissue, human colon tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells
Positive FC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:150-1:600 for WB
Immunohistochemistry (IHC)IHC : 1:100-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

11451-1-AP targets NQO1 in FC, IF, IHC, IP, WB, ELISA applications and shows reactivity with human samples.

Reactivity human
Cited Species calf, human, pig, swine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NQO1 fusion protein Ag2009
Full Name NAD(P)H dehydrogenase, quinone 1
Calculated molecular weight 274aa,31 kDa
Observed molecular weight 31 kDa
GenBank accession number BC007659
Gene symbol NQO1
Gene ID (NCBI) 1728
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20oC. Aliquoting is unnecessary for -20oC storage.

Background Information

NQO1, also named as DIA4, NMOR1, DTD and QR1, belongs to the NAD(P)H dehydrogenase (quinone) family. This enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis. It is known to be involved in benzene metabolism. In human studies of ozone exposure, polymorphisms in oxidative stress genes (NQO1, GSTM1, GSTP1) modify respiratory symptoms, lung function, biomarkers and risk of asthma. (PMID:18511640; 18848868 ) This antibody recognizes all the three isoforms (26-27 kDa and 31 kDa) of NQO1 and the homo-dimer form (66-70 kDa) of NQO1.

Protocols

Product Specific Protocols
WB protocol for NQO1 antibody 11451-1-APDownload protocol
IHC protocol for NQO1 antibody 11451-1-APDownload protocol
IF protocol for NQO1 antibody 11451-1-APDownload protocol
IP protocol for NQO1 antibody 11451-1-APDownload protocol
FC protocol for NQO1 antibody 11451-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancer Lett

NAD(P)H: Quinone oxidoreductase 1 overexpression in hepatocellular carcinoma potentiates apoptosis evasion through regulating stabilization of X-linked inhibitor of apoptosis protein.

Authors - Wan-Yu Li
  • KD Validated
ratWB

J Sci Food Agric

l-Methionine activates Nrf2-ARE pathway to induce endogenous antioxidant activity for depressing ROS-derived oxidative stress in growing rats.

Authors - Zhengxuan Wang
ratWB

Molecules

Neuroprotective Effect of Dioscin on the Aging Brain.

Authors - Yan Qi
mouseWB

J Agric Food Chem

Comparison of the Hepatoprotective Effects of the Three Main Stilbenes from Mulberry Twigs.

Authors - Ya-Nan Jia
humanWB

ACS Chem Neurosci

Selenepezil, a Selenium-Containing Compound, Exerts Neuroprotective Effect via Modulation of the Keap1-Nrf2-ARE Pathway and Attenuates Aβ-Induced Cognitive Impairment in Vivo.

Authors - Jun Yan
mouseWB

Biochem Biophys Res Commun

Curcumin upregulates the Nrf2 system by repressing inflammatory signaling-mediated Keap1 expression in insulin-resistant conditions.

Authors - Liwei Ren