Validation Data Gallery
|Positive WB detected in||K-562 cells, HepG2 cells|
|Positive IP detected in||HepG2 cells|
|Positive IHC detected in||human colon cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||MCF-7 cells|
|Positive FC detected in||MCF-7 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:150-1:600 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
11451-1-AP targets NQO1 in WB, IP, IHC, IF, FC applications and shows reactivity with human samples.
|Cited Reactivity||bovine, human, pig, zebrafish|
|Host / Isotype||Rabbit / IgG|
|Immunogen||NQO1 fusion protein Ag2009|
|Full Name||NAD(P)H dehydrogenase, quinone 1|
|Calculated molecular weight||274 aa, 31 kDa|
|Observed molecular weight||31 kDa|
|GenBank accession number||BC007659|
|Gene ID (NCBI)||1728|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
NQO1, also named as DIA4, NMOR1, DTD and QR1, belongs to the NAD(P)H dehydrogenase (quinone) family. This enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis. It is known to be involved in benzene metabolism. In human studies of ozone exposure, polymorphisms in oxidative stress genes (NQO1, GSTM1, GSTP1) modify respiratory symptoms, lung function, biomarkers and risk of asthma. (PMID:18511640; 18848868 ) This antibody recognizes all the three isoforms (26-27 kDa and 31 kDa) of NQO1 and the homo-dimer form (66-70 kDa) of NQO1.
|Product Specific Protocols|
|WB protocol for NQO1 antibody 11451-1-AP||Download protocol|
|IHC protocol for NQO1 antibody 11451-1-AP||Download protocol|
|IF protocol for NQO1 antibody 11451-1-AP||Download protocol|
|IP protocol for NQO1 antibody 11451-1-AP||Download protocol|
|Click here to view our Standard Protocols|
NAD(P)H: Quinone oxidoreductase 1 overexpression in hepatocellular carcinoma potentiates apoptosis evasion through regulating stabilization of X-linked inhibitor of apoptosis protein.
J Sci Food Agric
l-Methionine activates Nrf2-ARE pathway to induce endogenous antioxidant activity for depressing ROS-derived oxidative stress in growing rats.
Neuroprotective Effect of Dioscin on the Aging Brain.
J Agric Food Chem
Comparison of the Hepatoprotective Effects of the Three Main Stilbenes from Mulberry Twigs.
ACS Chem Neurosci
Selenepezil, a Selenium-Containing Compound, Exerts Neuroprotective Effect via Modulation of the Keap1-Nrf2-ARE Pathway and Attenuates Aβ-Induced Cognitive Impairment in Vivo.
Biochem Biophys Res Commun
Curcumin upregulates the Nrf2 system by repressing inflammatory signaling-mediated Keap1 expression in insulin-resistant conditions.