|Positive WB detected in||HL-60 cells, Sp2/0 cells, rat colon tissue, COLO 320 cells, SH-SY5Y cells, THP-1 cells, mouse liver tissue, rat liver tissue|
|Western Blot (WB)||WB : 1:2000-1:10000|
|Sample-dependent, check data in validation data gallery|
14351-1-AP targets NR1H3 in WB, IP, IHC, IF, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||NR1H3 fusion protein Ag5697|
|Full Name||nuclear receptor subfamily 1, group H, member 3|
|Calculated molecular weight||50 kDa|
|Observed molecular weight||45-50 kDa|
|GenBank accession number||BC041172|
|Gene ID (NCBI)||10062|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
NR1H3, also known as LXRA, is a liver X receptors, which form a subfamily of the nuclear receptor superfamily and are key regulators of macrophage function, controlling transcriptional programs involved in lipid homeostasis and inflammation. It's also a ligand-activated LXRs and forms heterodimers with retinoid X receptor, then participates in regulating expression of target genes containing LXR response elements. NR1H3 exists some isoforms with MV 51 kDa and 43-45 kDa.
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
K (Verified Customer) (06-10-2021)
works good in rat and human cells form western blotting.