NR1H3 Polyclonal antibody

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  WB analysis using 14351-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14351-1-AP (NR1H3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 14351-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 14351-1-AP (NR1H3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of rat liver using 14351-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 14351-1-AP (NR1H3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat liver using 14351-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 14351-1-AP (NR1H3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

NR1H3 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 14351-1-AP

Publications(96)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF, IP, ChIP, ELISA

Synonyms

RLD 1, Oxysterols receptor LXR alpha, LXRα, LXRA, LXR a

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Tested Applications

• Positive WB detected in: HL-60 cells, COLO 320 cells, mouse liver tissue, rat liver tissue, THP-1 cells
• Positive IHC detected in: rat liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 10 publications below
• WB: See 94 publications below
• IHC: See 6 publications below
• IF: See 3 publications below
• IP: See 1 publications below
• ChIP: See 1 publications below

Product Information

14351-1-AP targets NR1H3 in WB, IHC, IF, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, rabbit
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NR1H3 fusion protein Ag5697
• Full Name: nuclear receptor subfamily 1, group H, member 3
• Calculated Molecular Weight: 50 kDa
• Observed Molecular Weight: 45-50 kDa
• GenBank Accession Number: BC041172
• Gene Symbol: NR1H3
• Gene ID (NCBI): 10062
• RRID: AB_10640525
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q13133
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NR1H3, also known as LXRA, is a liver X receptors, which form a subfamily of the nuclear receptor superfamily and are key regulators of macrophage function, controlling transcriptional programs involved in lipid homeostasis and inflammation. It's also a ligand-activated LXRs and forms heterodimers with retinoid X receptor, then participates in regulating expression of target genes containing LXR response elements. NR1H3 exists some isoforms with MV 51 kDa and 43-45 kDa.

Publications

Species	Application	Title
	WB	Nat Commun; p21-activated kinase 4 suppresses fatty acid β-oxidation and ketogenesis by phosphorylating NCoR1; Authors - Min Yan Shi
human	WB	Br J Pharmacol; Functional interplay between LXR and AMPKα inhibits atherosclerosis in apoE deficient mice - A new anti-atherogenic strategy.; Authors - Chuanrui Ma
mouse	WB	Theranostics; Encapsulation of LXR ligand by D-Nap-GFFY hydrogel enhances anti-tumorigenic actions of LXR and removes LXR-induced lipogenesis.; Authors - Ke Feng
human	WB	EMBO Rep; Lin28 enhances de novo fatty acid synthesis to promote cancer progression via SREBP-1.; Authors - Yang Zhang
mouse	WB,IF	Food Res Int; Cabernet sauvignon dry red wine ameliorates atherosclerosis in mice by regulating inflammation and endothelial function, activating AMPK phosphorylation, and modulating gut microbiota; Authors - Xinlong Cheng
human,mouse	WB	Phytomedicine; Rice bran active peptide (RBAP) inhibited macrophage differentiation to foam cell and atherosclerosis in mice via regulating cholesterol efflux; Authors - Jianfei Mu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

K (Verified Customer) (06-10-2021)

works good in rat and human cells form western blotting.

• Applications: Western Blot
• Primary Antibody Dilution: 1;1000
• Cell Tissue Type: rat and human liver cells