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NR1H3 Polyclonal antibody
NR1H3 Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IP, IHC, IF, ChIP, ELISA
Conjugate
Unconjugated
Cat no : 14351-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HL-60 cells, Sp2/0 cells, rat colon tissue, COLO 320 cells, SH-SY5Y cells, THP-1 cells, mouse liver tissue, rat liver tissue |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 10 publications below |
WB | See 74 publications below |
IHC | See 3 publications below |
IF | See 3 publications below |
IP | See 1 publications below |
ChIP | See 1 publications below |
Product Information
14351-1-AP targets NR1H3 in WB, IP, IHC, IF, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, pig |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | NR1H3 fusion protein Ag5697 |
Full Name | nuclear receptor subfamily 1, group H, member 3 |
Calculated molecular weight | 50 kDa |
Observed molecular weight | 45-50 kDa |
GenBank accession number | BC041172 |
Gene symbol | NR1H3 |
Gene ID (NCBI) | 10062 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
NR1H3, also known as LXRA, is a liver X receptors, which form a subfamily of the nuclear receptor superfamily and are key regulators of macrophage function, controlling transcriptional programs involved in lipid homeostasis and inflammation. It's also a ligand-activated LXRs and forms heterodimers with retinoid X receptor, then participates in regulating expression of target genes containing LXR response elements. NR1H3 exists some isoforms with MV 51 kDa and 43-45 kDa.
Protocols
Product Specific Protocols | |
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WB protocol for NR1H3 antibody 14351-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nat Commun p21-activated kinase 4 suppresses fatty acid β-oxidation and ketogenesis by phosphorylating NCoR1 | ||
Br J Pharmacol Functional interplay between LXR and AMPKα inhibits atherosclerosis in apoE deficient mice - A new anti-atherogenic strategy. | ||
Theranostics Encapsulation of LXR ligand by D-Nap-GFFY hydrogel enhances anti-tumorigenic actions of LXR and removes LXR-induced lipogenesis. | ||
EMBO Rep Lin28 enhances de novo fatty acid synthesis to promote cancer progression via SREBP-1. | ||
Food Res Int Cabernet sauvignon dry red wine ameliorates atherosclerosis in mice by regulating inflammation and endothelial function, activating AMPK phosphorylation, and modulating gut microbiota | ||
Br J Pharmacol Targeting macrophage liver X receptors by hydrogel-encapsulated T0901317 reduces atherosclerosis without effect on hepatic lipogenesis. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH K (Verified Customer) (06-10-2021) | works good in rat and human cells form western blotting.
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