NR1H3 Polyclonal antibody

• Featured Product
• KD/KO Validated

NR1H3 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, ChIP, ELISA

Conjugate

Unconjugated

Publications

75

Reviews (1)

Cat no : 14351-1-AP

Synonyms

Liver X receptor alpha, LXR a, LXRA, LXRα, NR1H3, Oxysterols receptor LXR alpha, RLD 1

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Validation Data Gallery

• 

  WB analysis using 14351-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14351-1-AP (NR1H3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14351-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14351-1-AP (NR1H3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 14351-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 14351-1-AP (NR1H3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat colon using 14351-1-AP

  rat colon tissue were subjected to SDS PAGE followed by western blot with 14351-1-AP (NR1H3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Sp2/0 using 14351-1-AP

  Sp2/0 cells were subjected to SDS PAGE followed by western blot with 14351-1-AP (NR1H3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HL-60 cells, Sp2/0 cells, rat colon tissue, COLO 320 cells, SH-SY5Y cells, THP-1 cells, mouse liver tissue, rat liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 10 publications below
• WB: See 74 publications below
• IHC: See 3 publications below
• IF: See 3 publications below
• IP: See 1 publications below
• ChIP: See 1 publications below

Product Information

14351-1-AP targets NR1H3 in WB, IP, IHC, IF, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NR1H3 fusion protein Ag5697
• Full Name: nuclear receptor subfamily 1, group H, member 3
• Calculated molecular weight: 50 kDa
• Observed molecular weight: 45-50 kDa
• GenBank accession number: BC041172
• Gene symbol: NR1H3
• Gene ID (NCBI): 10062
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NR1H3, also known as LXRA, is a liver X receptors, which form a subfamily of the nuclear receptor superfamily and are key regulators of macrophage function, controlling transcriptional programs involved in lipid homeostasis and inflammation. It's also a ligand-activated LXRs and forms heterodimers with retinoid X receptor, then participates in regulating expression of target genes containing LXR response elements. NR1H3 exists some isoforms with MV 51 kDa and 43-45 kDa.

Publications

Species	Application	Title
	WB	Nat Commun; p21-activated kinase 4 suppresses fatty acid β-oxidation and ketogenesis by phosphorylating NCoR1; Authors - Min Yan Shi
human	WB	Br J Pharmacol; Functional interplay between LXR and AMPKα inhibits atherosclerosis in apoE deficient mice - A new anti-atherogenic strategy.; Authors - Chuanrui Ma
mouse	WB	Theranostics; Encapsulation of LXR ligand by D-Nap-GFFY hydrogel enhances anti-tumorigenic actions of LXR and removes LXR-induced lipogenesis.; Authors - Ke Feng
human	WB	EMBO Rep; Lin28 enhances de novo fatty acid synthesis to promote cancer progression via SREBP-1.; Authors - Yang Zhang
mouse	WB,IF	Food Res Int; Cabernet sauvignon dry red wine ameliorates atherosclerosis in mice by regulating inflammation and endothelial function, activating AMPK phosphorylation, and modulating gut microbiota; Authors - Xinlong Cheng
mouse	WB	Br J Pharmacol; Targeting macrophage liver X receptors by hydrogel-encapsulated T0901317 reduces atherosclerosis without effect on hepatic lipogenesis.; Authors - Chuanrui Ma

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

K (Verified Customer) (06-10-2021)

works good in rat and human cells form western blotting.

• Applications: Western Blot
• Primary Antibody Dilution: 1;1000
• Cell Tissue Type: rat and human liver cells

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