NR1H3 Monoclonal antibody

NR1H3 Monoclonal Antibody for IF, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, pig and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

2

CloneNo.

4A4F7

Cat no : 60134-1-Ig

Synonyms

Liver X receptor alpha, LXR a, LXRA, NR1H3, Oxysterols receptor LXR alpha, RLD 1

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Validation Data Gallery

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  WB analysis using 60134-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60134-1-Ig (NR1H3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 60134-1-Ig

  MCF7 cells were subjected to SDS PAGE followed by western blot with 60134-1-Ig (NR1H3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 60134-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 60134-1-Ig (NR1H3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 60134-1-Ig

  Immunofluorescent analysis of HepG2 cells using 60134-1-Ig (NR1H3 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).

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Tested Applications

• Positive WB detected in: T-47D cells, HepG2 cells, MCF-7 cells, COLO 320 cells
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

60134-1-Ig targets NR1H3 in WB, IHC, IF, ELISA applications and shows reactivity with human, rat, pig samples.

• Tested Reactivity: human, rat, pig
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: NR1H3 fusion protein Ag6184
• Full Name: nuclear receptor subfamily 1, group H, member 3
• Calculated Molecular Weight: 50 kDa
• Observed Molecular Weight: 44 kDa
• GenBank Accession Number: BC041172
• Gene Symbol: NR1H3
• Gene ID (NCBI): 10062
• RRID: AB_2154887
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NR1H3, also known as LXRA, is a liver X receptors, which form a subfamily of the nuclear receptor superfamily and are key regulators of macrophage function, controlling transcriptional programs involved in lipid homeostasis and inflammation. It's also a ligand-activated LXRs and forms heterodimers with retinoid X receptor, then participates in regulating expression of target genes containing LXR response elements.

Publications

Species	Application	Title
mouse	WB	Food Funct; CD36 and DGAT2 facilitate the lipid-lowering effect of chitooligosaccharides via fatty acid intake and triglyceride synthesis signaling.; Authors - Xin Shen
human	IHC	J Transl Med; Multiplex immunohistochemistry defines two cholesterol metabolism patterns predicting immunotherapeutic outcomes in gastric cancer; Authors - Wei Tang