NR1H3 Monoclonal antibody

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• NR1H3 Monoclonal antibody (60134-1-Ig)

NR1H3 Monoclonal Antibody for WB, ELISA

Cat No. 60134-1-Ig Copied!

Clone No. 4A4F7

Publications (3)

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Host / Isotype

Mouse / IgG1

Reactivity

human, rat, pig and More (1)

Applications

WB, IHC, IF, ELISA

Synonyms

Oxysterols receptor LXR alpha, LXRα, LXRA, LXR a, Liver X receptor alpha

• 

  WB analysis using 60134-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60134-1-Ig (NR1H3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of MCF-7 using 60134-1-Ig

  MCF7 cells were subjected to SDS PAGE followed by western blot with 60134-1-Ig (NR1H3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HepG2 using 60134-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 60134-1-Ig (NR1H3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: T-47D cells, HepG2 cells, MCF-7 cells, COLO 320 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

60134-1-Ig targets NR1H3 in WB, IHC, IF, ELISA applications and shows reactivity with human, rat, pig samples.

• Tested Reactivity: human, rat, pig
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag6184

  Product name: Recombinant human NR1H3 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 104-402 aa of BC041172

  Sequence: KCQECRLRKCRQAGMREECVLSEEQIRLKKLKRQEEEQAHATSLPPRASSPPQILPQLSPEQLGMIEKLVAAQQQCNRRSFSDRLRVTPWPMAPDPHSREARQQRFAHFTELAIVSVQEIVDFAKQLPGFLQLSREDQIALLKTSAIEVMLLETSRRYNPGSESITFLKDFSYNREDFAKAGLQVEFINPIFEFSRAMNELQLNDAEFALLIAISIFSADRPNVQDQLQVERLQHTYVEALHAYVSIHHPHDRLMFPRMLMKLVSLRTLSSVHSEQVFALRLQDKKLPPLLSEIWDVHE

• Full Name: nuclear receptor subfamily 1, group H, member 3
• Calculated Molecular Weight: 50 kDa
• Observed Molecular Weight: 44 kDa
• GenBank Accession Number: BC041172
• Gene Symbol: NR1H3
• Gene ID (NCBI): 10062
• RRID: AB_2154887
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q13133
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NR1H3, also known as LXRA, is a liver X receptors, which form a subfamily of the nuclear receptor superfamily and are key regulators of macrophage function, controlling transcriptional programs involved in lipid homeostasis and inflammation. It's also a ligand-activated LXRs and forms heterodimers with retinoid X receptor, then participates in regulating expression of target genes containing LXR response elements.

Publications

What published studies show

The NR1H3 Monoclonal antibody (4A4F7), catalog number 60134-1-Ig, has a total of 3 citations. These appear in Journal of Translational Medicine, Food & Function, and Respiratory Research. The associated research fields include cholesterol and lipid metabolism in gastric cancer immunotherapy, fatty acid uptake and triglyceride synthesis signaling, and LXR signaling in bronchopulmonary dysplasia.

Automatically generated from the 3 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IHC	J Transl Med; Multiplex immunohistochemistry defines two cholesterol metabolism patterns predicting immunotherapeutic outcomes in gastric cancer; Authors - Wei Tang
mouse	WB	Food Funct; CD36 and DGAT2 facilitate the lipid-lowering effect of chitooligosaccharides via fatty acid intake and triglyceride synthesis signaling.; Authors - Xin Shen
mouse	WB,IF	Respir Res; Activation of LXR signaling ameliorates apoptosis of alveolar epithelial cells in Bronchopulmonary dysplasia; Authors - Yizhe Ma