|Positive WB detected in||HepG2 cells, MCF-7 cells, MDA-MB-231 cells|
|Positive IHC detected in||human kidney tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:1000-1:6000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of 25055-1-AP is NR1H4 Fusion Protein expressed in E. coli.
|Cited Reactivity||mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||NR1H4 fusion protein Ag21878|
|Full Name||nuclear receptor subfamily 1, group H, member 4|
|Calculated molecular weight||486 aa, 56 kDa|
|Observed molecular weight||48 kDa|
|GenBank accession number||BC130573|
|Gene ID (NCBI)||9971|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Nuclear Receptor subfamily 1, group H, member 4 (NR1H4, also known as FXR) is a receptor for bile acids and has an important role in regulating energy metabolism in liver, muscle and adipose tissues in humans and animals. NR1H4 is highly expressed in liver and has a role in lipid metabolism, whereas none of the other protein-coding genes in the region has known biological links with cholesterol, further supporting a role for NR1H4 in regulation of cholesterol levels. NR1H4 have four isoforms, i.e., FXRalpha1, FXRalpha2, FXRbeta1, and FXRbeta2, and each isoform has a different expressional level and transcriptional activity depending on its location within specific tissues. (PMID: 14733360, PMID: 30787420)
Protective effect of dioscin against thioacetamide-induced acute liver injury via FXR/AMPK signaling pathway in vivo.
Toxicol Appl Pharmacol
Picroside II alleviates liver injury induced by alpha-naphthylisothiocyanate through AMPK-FXR pathway.
Probiotic-fermented black tartary buckwheat alleviates hyperlipidemia and gut microbiota dysbiosis in rats fed with a high-fat diet.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Bryce (Verified Customer) (04-16-2021)
Antibody did not work for mouse kidney homogenates. There was no observable difference between kidneys from wild type and knockout mice. Genotypes of blotted samples were confirmed by RT-qPCR.