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  • KD/KO Validated

NR1H4 Polyclonal antibody

NR1H4 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 25055-1-AP

Synonyms

BAR, Bile acid receptor, Farnesoid X activated receptor, Farnesol receptor HRR 1, FXR, HRR 1, HRR1, NR1H4, RIP14, RXR interacting protein 14



Tested Applications

Positive WB detected inHepG2 cells, MCF-7 cells, MDA-MB-231 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 25055-1-AP is NR1H4 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NR1H4 fusion protein Ag21878
Full Name nuclear receptor subfamily 1, group H, member 4
Calculated molecular weight 486 aa, 56 kDa
Observed molecular weight 48 kDa
GenBank accession numberBC130573
Gene symbol NR1H4
Gene ID (NCBI) 9971
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Nuclear Receptor subfamily 1, group H, member 4 (NR1H4, also known as FXR) is a receptor for bile acids and has an important role in regulating energy metabolism in liver, muscle and adipose tissues in humans and animals. NR1H4 is highly expressed in liver and has a role in lipid metabolism, whereas none of the other protein-coding genes in the region has known biological links with cholesterol, further supporting a role for NR1H4 in regulation of cholesterol levels. NR1H4 have four isoforms, i.e., FXRalpha1, FXRalpha2, FXRbeta1, and FXRbeta2, and each isoform has a different expressional level and transcriptional activity depending on its location within specific tissues. (PMID: 14733360, PMID: 30787420)

Protocols

Product Specific Protocols
WB protocol for NR1H4 antibody 25055-1-APDownload protocol
IHC protocol for NR1H4 antibody 25055-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IHC

Biomater Sci

Mesenchymal stem cell-derived exosome mitigates colitis via the modulation of the gut metagenomics-metabolomics-farnesoid X receptor axis

Authors - Dickson Kofi Wiredu Ocansey
mouseWB

Food Chem Toxicol

Gut microbiota mediates methamphetamine-induced hepatic inflammation via the impairment of bile acid homeostasis.

Authors - Kai-Kai Zhang
human,ratWB

Phytomedicine

Dolomiaea souliei ethyl acetate extract protected against α-naphthylisothiocyanate-induced acute intrahepatic cholestasis through regulation of farnesoid x receptor-mediated bile acid metabolism

Authors - FanCheng Meng
mouseWB

Front Pharmacol

Hepatoprotective Effects of Glycyrrhetinic Acid on Lithocholic Acid-Induced Cholestatic Liver Injury Through Choleretic and Anti-Inflammatory Mechanisms.

Authors - Qian Wang
ratWB

Food Funct

Probiotic-fermented black tartary buckwheat alleviates hyperlipidemia and gut microbiota dysbiosis in rats fed with a high-fat diet.

Authors - Yunhong Ren
mouseWB,IF

Front Endocrinol (Lausanne)

DMRT2 Interacts With FXR and Improves Insulin Resistance in Adipocytes and a Mouse Model.

Authors - Jing Tao
  • KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Bryce (Verified Customer) (04-16-2021)

Antibody did not work for mouse kidney homogenates. There was no observable difference between kidneys from wild type and knockout mice. Genotypes of blotted samples were confirmed by RT-qPCR.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: mouse kidney
NR1H4 Antibody Western Blot validation (1:1000 dilution) in mouse kidney (Cat no:25055-1-AP)