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  • KD/KO Validated

NR2E3 Polyclonal antibody

NR2E3 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig and More (1)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 14246-1-AP

Synonyms

ESCS, NR2E3, PNR, rd7, RNR, RP37



Tested Applications

Positive WB detected inmouse retina tissue, Y79 cells, HepG2 cells, rat retina tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inphotoreceptor progenitor cells
Positive FC detected inphotoreceptor progenitor cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:100
Flow Cytometry (FC)FC : ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

14246-1-AP targets NR2E3 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Cited Reactivityhuman, mouse, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NR2E3 fusion protein Ag5503
Full Name nuclear receptor subfamily 2, group E, member 3
Calculated molecular weight 45 kDa
Observed molecular weight 43-45 kDa
GenBank accession numberBC041421
Gene symbol NR2E3
Gene ID (NCBI) 10002
RRIDAB_2155476
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NR2E3, also known as PNR, encodes a retinal nuclear receptor that is a ligand-dependent transcription factor. This protein is part of a large family of nuclear receptor transcription factors involved in signaling pathways. NR2E3 influences the development of photoreceptors and their differentiation into rod and cone types, and acts as a ranscriptional factor that is an activator of rod development and repressor of cone development [PMID:20725840]. It binds the promoter region of a number of rod- and cone-specific genes, including rhodopsin, M- and S-opsin and rod-specific phosphodiesterase beta subunit. [PMID:15689355]

Protocols

Product Specific Protocols
WB protocol for NR2E3 antibody 14246-1-APDownload protocol
IHC protocol for NR2E3 antibody 14246-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

PLoS Genet

Distinct and Atypical Intrinsic and Extrinsic Cell Death Pathways between Photoreceptor Cell Types upon Specific Ablation of Ranbp2 in Cone Photoreceptors.

Authors - Cho Kyoung-In KI
mouseWB

J Biol Chem

Differential Loss of Prolyl-Isomerase or Chaperone Activity of Ran-binding protein 2 (Ranbp2) Unveils Distinct Physiological Roles of Its Cyclophilin Domain in Proteostasis.

Authors - Kyoung-In Cho
humanWB

Sci Rep

Loss of NR2E3 represses AHR by LSD1 reprogramming, is associated with poor prognosis in liver cancer.

Authors - Tilak Khanal
mouseWB

FASEB J

NR2E3 is a key component in p53 activation by regulating a long noncoding RNA DINO in acute liver injuries.

Authors - Tilak Khanal
  • KO Validated
zebrafishIHC

Am J Pathol

Dysregulation of Spliceosomes Complex Induces Retinitis Pigmentosa-Like Characteristics in sf3b4-Depleted Zebrafish

Authors - Zulvikar Syambani Ulhaq