• Featured Product
  • KD/KO Validated

NR2E3 Polyclonal antibody

NR2E3 Polyclonal Antibody for FC, IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF, FC,ELISA

Conjugate

Unconjugated

Cat no : 14246-1-AP

Synonyms

ESCS, NR2E3, PNR, rd7, RNR, RP37



Tested Applications

Positive WB detected inY79 cells, HepG2 cells, mouse eye tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inphotoreceptor progenitor cells
Positive FC detected inphotoreceptor progenitor cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:100
Sample-dependent, check data in validation data gallery

Product Information

14246-1-AP targets NR2E3 in WB, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NR2E3 fusion protein Ag5503
Full Name nuclear receptor subfamily 2, group E, member 3
Calculated molecular weight 45 kDa
Observed molecular weight 43-45 kDa
GenBank accession numberBC041421
Gene symbol NR2E3
Gene ID (NCBI) 10002
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NR2E3, also known as PNR, encodes a retinal nuclear receptor that is a ligand-dependent transcription factor. This protein is part of a large family of nuclear receptor transcription factors involved in signaling pathways. NR2E3 influences the development of photoreceptors and their differentiation into rod and cone types, and acts as a ranscriptional factor that is an activator of rod development and repressor of cone development [PMID:20725840]. It binds the promoter region of a number of rod- and cone-specific genes, including rhodopsin, M- and S-opsin and rod-specific phosphodiesterase beta subunit. [PMID:15689355]

Protocols

Product Specific Protocols
WB protocol for NR2E3 antibody 14246-1-APDownload protocol
IHC protocol for NR2E3 antibody 14246-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

FASEB J

NR2E3 is a key component in p53 activation by regulating a long noncoding RNA DINO in acute liver injuries.

Authors - Tilak Khanal
  • KO Validated
humanWB

Sci Rep

Loss of NR2E3 represses AHR by LSD1 reprogramming, is associated with poor prognosis in liver cancer.

Authors - Tilak Khanal
mouseWB

J Biol Chem

Differential Loss of Prolyl-Isomerase or Chaperone Activity of Ran-binding protein 2 (Ranbp2) Unveils Distinct Physiological Roles of Its Cyclophilin Domain in Proteostasis.

Authors - Kyoung-In Cho
mouseIHC

PLoS Genet

Distinct and Atypical Intrinsic and Extrinsic Cell Death Pathways between Photoreceptor Cell Types upon Specific Ablation of Ranbp2 in Cone Photoreceptors.

Authors - Cho Kyoung-In KI