• Featured Product
  • KD/KO Validated

NR5A2 Polyclonal antibody

NR5A2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (1)





Cat no : 22460-1-AP


B1 binding factor, B1F, B1F2, CPF, CYP7A promoter binding factor, FTF, FTZ F1, FTZ F1beta, hB1F, hB1F 2, Liver receptor homolog 1, LRH 1, NR5A2

Tested Applications

Positive WB detected inA549 cells, rat testis tissue, mouse ovary tissue, BxPC-3 cells, mouse liver tissue, mouse pancreas tissue

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22460-1-AP targets NR5A2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, sheep
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NR5A2 fusion protein Ag18001
Full Name nuclear receptor subfamily 5, group A, member 2
Calculated Molecular Weight 541 aa, 61 kDa
Observed Molecular Weight 61-67 kDa
GenBank Accession NumberBC118652
Gene Symbol NR5A2
Gene ID (NCBI) 2494
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NR5A2 belongs to the nuclear hormone receptor family NR5 subfamily.It binds to the sequence element 5'-AACGACCGACCTTGAG-3' of the enhancer II of hepatitis B virus genes, a critical cis-element of their expression and regulation.NR5A2 may be responsible for the liver-specific activity of enhancer II, probably in combination with other hepatocyte transcription factors. Key regulator of cholesterol 7-alpha-hydroxylase gene (CYP7A) expression in liver. It may also contribute to the regulation of pancreas-specific genes and play important roles in embryonic development. LRH-1 is phosphorylated to display its normal function, including its role in the intriguing potential responses to phospholipid or other ligands. The hinge domain serine residues at 238 and 243 are required for effective phosphorylation, both in vitro and in cells (16439367).NR5A1 is abundantly expressed in pancreas, less in liver, very low levels in heart and lung. Expressed in hepG2. Isoform 1 and isoform 2 seem to be present in fetal and adult liver and hepG2 cells.The observed molecular weight of 65-70 kDa is the posphorylation of NR5A2 protein.


Product Specific Protocols
WB protocol for NR5A2 antibody 22460-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Aging (Albany NY)

NR5A2 as a potential target for exercise to improve metabolic syndrome

Authors - Lingxiu Meng

Cell Prolif

LRH-1 activation alleviates diabetes-induced podocyte injury by promoting GLS2-mediated glutaminolysis

Authors - Jijia Hu

Mol Ther Nucleic Acids

MicroRNA-3613-5p Promotes Lung Adenocarcinoma Cell Proliferation through a RELA and AKT/MAPK Positive Feedback Loop.

Authors - Tao He
  • KD Validated

Pharmacol Res

Geniposide reduces cholesterol accumulation and increases its excretion by regulating the FXR-mediated liver-gut crosstalk of bile acids.

Authors - Jinxin Liu

Front Oncol

NR5A2 Is One of 12 Transcription Factors Predicting Prognosis in HNSCC and Regulates Cancer Cell Proliferation in a p53-Dependent Manner.

Authors - Kun Zhang
  • KD Validated
humanWB, IHC

Cell Death Discov

NR5A2 transcriptional activation by BRD4 promotes pancreatic cancer progression by upregulating GDF15.

Authors - Feng Guo