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NRF1/nuclear respiratory factor 1 Polyclonal antibody

NRF1/nuclear respiratory factor 1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 12482-1-AP

Synonyms

ALPHA PAL, NRF 1, NRF1, NRF1/nuclear respiratory factor 1, nuclear respiratory factor 1



Tested Applications

Positive WB detected inA549 cells, human lung tissue, A431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Sample-dependent, check data in validation data gallery

Product Information

12482-1-AP targets NRF1/nuclear respiratory factor 1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, chicken, ducks, goat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NRF1/nuclear respiratory factor 1 fusion protein Ag3172
Full Name nuclear respiratory factor 1
Calculated molecular weight 522 aa, 56 kDa
Observed molecular weight 67 kDa, 45 kDa
GenBank accession numberBC016925
Gene symbol NRF1
Gene ID (NCBI) 4899
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NRF1, also named as Alpha-pal and NRF-1, belongs to the NRF1/Ewg family. It is a transcription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene. NRF1 is implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. It plays a critical role in regulating the expression of many antioxidant response element (ARE)-dependent genes. NRF1 exists various isoforms and long isoform is about 67 kDa and short isoform is about 45 kDa.

Protocols

Product Specific Protocols
WB protocol for NRF1/nuclear respiratory factor 1 antibody 12482-1-APDownload protocol
IP protocol for NRF1/nuclear respiratory factor 1 antibody 12482-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nature

A plant-derived natural photosynthetic system for improving cell anabolism

Authors - Pengfei Chen
humanWB

Mater Today Bio

Platelet-derived extracellular vesicles ameliorate intervertebral disc degeneration by alleviating mitochondrial dysfunction

Authors - Zhanqiu Dai
humanWB

Redox Biol

Defective mitochondrial ISCs biogenesis switches on IRP1 to fine tune selective mitophagy.

Authors - Hao Wu
mouseWB

EBioMedicine

DsbA-L interacts with VDAC1 in mitochondrion-mediated tubular cell apoptosis and contributes to the progression of acute kidney disease.

Authors - Xiaozhou Li
mouseWB

Aging (Albany NY)

Folic acid delays age-related cognitive decline in senescence-accelerated mouse prone 8: alleviating telomere attrition as a potential mechanism.

Authors - Xin Lv
ratWB

Aging (Albany NY)

Spermidine alleviates cardiac aging by improving mitochondrial biogenesis and function.

Authors - Junying Wang