NRF1/nuclear respiratory factor 1 Polyclonal antibody

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• NRF1/nuclear respiratory factor 1 Polyclonal antibody (12482-1-AP)

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NRF1/nuclear respiratory factor 1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 12482-1-AP

Publications(143)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (6)

Applications

WB, IHC, IF, ELISA

Synonyms

NRF1, nuclear respiratory factor 1, ALPHA PAL, Alpha palindromic-binding protein, Alpha-pal

• 

  WB analysis using 12482-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12482-1-AP (NRF1/nuclear respiratory factor 1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human lung using 12482-1-AP

  human lung tissue were subjected to SDS PAGE followed by western blot with 12482-1-AP (NRF1/nuclear respiratory factor 1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of mouse skeletal muscle using 12482-1-AP

  IP result of anti-NRF1/nuclear respiratory factor 1 (IP:12482-1-AP, 4ug; Detection:12482-1-AP 1:300) with mouse skeletal muscle tissue lysate 3000ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human stomach using 12482-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 12482-1-AP (NRF1/nuclear respiratory factor 1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: A549 cells, human lung tissue, HEK-293T cells
• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12482-1-AP targets NRF1/nuclear respiratory factor 1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, chicken, zebrafish, goat, duck, ducks
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3172

  Product name: Recombinant human NRF1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 213-522 aa of BC016925

  Sequence: LKYSTGRGKPGWGKESCKPIWWPEDIPWANVRSDVRTEEQKQRVSWTQALRTIVKNCYKQHGREDLLYAFEDQQTQTQATATHSIAHLVPSQTVVQTFSNPDGTVSLIQVGTGATVATLADASELPTTVTVAQVNYSAVADGEVEQNWATLQGGEMTIQTTQASEATQAVASLAEAAVAASQEMQQGATVTMALNSEAAAHAVATLAEATLQGGGQIVLSGETAAAVGALTGVQDANGLFMADRAGRKWILTDKATGLVQIPVSMYQTVVTSLAQGNGPVQVAMAPVTTRISDSAVTMDGQAVEVVTLEQ

• Full Name: nuclear respiratory factor 1
• Calculated Molecular Weight: 522 aa, 56 kDa
• Observed Molecular Weight: 67 kDa, 45 kDa
• GenBank Accession Number: BC016925
• Gene Symbol: NRF1
• Gene ID (NCBI): 4899
• RRID: AB_2282876
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q16656
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NRF1, also named as Alpha-pal and NRF-1, belongs to the NRF1/Ewg family. It is a transcription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene. NRF1 is implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. It plays a critical role in regulating the expression of many antioxidant response element (ARE)-dependent genes. NRF1 exists various isoforms and long isoform is about 67 kDa and short isoform is about 45 kDa.

Publications

What published studies show

Automatically generated from the publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Signal Transduct Target Ther; Short-chain acyl-CoA dehydrogenase initiates mtDNA demethylation and leakage to fuel antitumor immunity in colorectal cancer.; Authors - Fang Yang
mouse	WB	Nature; A plant-derived natural photosynthetic system for improving cell anabolism; Authors - Pengfei Chen
		Autophagy; Porcine reproductive and respiratory syndrome virus hijacks the non-canonical enzymatic function of PHGDH to arrest autophagic flux for viral replication.; Authors - Zhangping Yu
		Autophagy; HTLV-1 Tax induces PINK1-PRKN/parkin-dependent mitophagy to mitigate activation of the CGAS-STING1 pathway.; Authors - Suchitra Mohanty
human	WB,IF	Nat Commun; Single-cell exon deletion profiling reveals splicing events that shape gene expression and cell state dynamics.; Authors - Bandana Kumari
human	WB	Nat Commun; ZHX2 emerges as a negative regulator of mitochondrial oxidative phosphorylation during acute liver injury; Authors - Yankun Zhang