NRF1/nuclear respiratory factor 1 Polyclonal antibody

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  WB analysis using 12482-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12482-1-AP (NRF1/nuclear respiratory factor 1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of human lung using 12482-1-AP

  human lung tissue were subjected to SDS PAGE followed by western blot with 12482-1-AP (NRF1/nuclear respiratory factor 1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse skeletal muscle using 12482-1-AP

  IP result of anti-NRF1/nuclear respiratory factor 1 (IP:12482-1-AP, 4ug; Detection:12482-1-AP 1:300) with mouse skeletal muscle tissue lysate 3000ug.

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  IHC staining of human stomach using 12482-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 12482-1-AP (NRF1/nuclear respiratory factor 1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

NRF1/nuclear respiratory factor 1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 12482-1-AP

Publications(100)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (7)

Applications

WB, IHC, IF, ELISA

Synonyms

NRF1, nuclear respiratory factor 1, ALPHA PAL, Alpha palindromic-binding protein, Alpha-pal

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Tested Applications

• Positive WB detected in: A549 cells, human lung tissue, HEK-293T cells
• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 97 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

12482-1-AP targets NRF1/nuclear respiratory factor 1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, chicken, zebrafish, bovine, goat, duck, ducks
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3172

  Product name: Recombinant human NRF1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 213-522 aa of BC016925

  Sequence: LKYSTGRGKPGWGKESCKPIWWPEDIPWANVRSDVRTEEQKQRVSWTQALRTIVKNCYKQHGREDLLYAFEDQQTQTQATATHSIAHLVPSQTVVQTFSNPDGTVSLIQVGTGATVATLADASELPTTVTVAQVNYSAVADGEVEQNWATLQGGEMTIQTTQASEATQAVASLAEAAVAASQEMQQGATVTMALNSEAAAHAVATLAEATLQGGGQIVLSGETAAAVGALTGVQDANGLFMADRAGRKWILTDKATGLVQIPVSMYQTVVTSLAQGNGPVQVAMAPVTTRISDSAVTMDGQAVEVVTLEQ

• Full Name: nuclear respiratory factor 1
• Calculated Molecular Weight: 522 aa, 56 kDa
• Observed Molecular Weight: 67 kDa, 45 kDa
• GenBank Accession Number: BC016925
• Gene Symbol: NRF1
• Gene ID (NCBI): 4899
• RRID: AB_2282876
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q16656
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NRF1, also named as Alpha-pal and NRF-1, belongs to the NRF1/Ewg family. It is a transcription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene. NRF1 is implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. It plays a critical role in regulating the expression of many antioxidant response element (ARE)-dependent genes. NRF1 exists various isoforms and long isoform is about 67 kDa and short isoform is about 45 kDa.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Nature; A plant-derived natural photosynthetic system for improving cell anabolism; Authors - Pengfei Chen
chicken	WB,IHC	J Hazard Mater; Mitochondrial miR-12294-5p regulated copper-induced mitochondrial oxidative stress and mitochondrial quality control imbalance by targeted inhibition of CISD1 in chicken livers; Authors - Gaolong Zhong
	WB	J Autoimmun; Autoantibodies against the NineTeen complex and U5 RNP in systemic sclerosis; Authors - Jean-Baptiste Vulsteke
rat,human	WB	J Adv Res; A dual absorption pathway of novel oyster-derived peptide-zinc complex enhances zinc bioavailability and restores mitochondrial function; Authors - Ximing Yang
human	WB	Redox Biol; Defective mitochondrial ISCs biogenesis switches on IRP1 to fine tune selective mitophagy.; Authors - Hao Wu
chicken	WB	Cell Commun Signal; Local GHR roles in regulation of mitochondrial function through mitochondrial biogenesis during myoblast differentiation; Authors - Bowen Hu