NRF1/nuclear respiratory factor 1 Polyclonal antibody

• Featured Product
• KD/KO Validated

NRF1/nuclear respiratory factor 1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

52

Cat no : 12482-1-AP

Synonyms

ALPHA PAL, NRF 1, NRF1, NRF1/nuclear respiratory factor 1, nuclear respiratory factor 1

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Validation Data Gallery

• 

  WB analysis using 12482-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12482-1-AP (NRF1/nuclear respiratory factor 1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 12482-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 12482-1-AP (NRF1/nuclear respiratory factor 1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human lung using 12482-1-AP

  human lung tissue were subjected to SDS PAGE followed by western blot with 12482-1-AP (NRF1/nuclear respiratory factor 1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse skeletal muscle using 12482-1-AP

  IP Result of anti-NRF1/nuclear respiratory factor 1 (IP:12482-1-AP, 4ug; Detection:12482-1-AP 1:300) with mouse skeletal muscle tissue lysate 3000ug.

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Tested Applications

• Positive WB detected in: A549 cells, human lung tissue, A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 2 publications below
• WB: See 50 publications below

Product Information

12482-1-AP targets NRF1/nuclear respiratory factor 1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, chicken, ducks, goat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NRF1/nuclear respiratory factor 1 fusion protein Ag3172
• Full Name: nuclear respiratory factor 1
• Calculated molecular weight: 522 aa, 56 kDa
• Observed molecular weight: 67 kDa, 45 kDa
• GenBank accession number: BC016925
• Gene symbol: NRF1
• Gene ID (NCBI): 4899
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NRF1, also named as Alpha-pal and NRF-1, belongs to the NRF1/Ewg family. It is a transcription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene. NRF1 is implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. It plays a critical role in regulating the expression of many antioxidant response element (ARE)-dependent genes. NRF1 exists various isoforms and long isoform is about 67 kDa and short isoform is about 45 kDa.

Publications

Species	Application	Title
mouse	WB	Nature; A plant-derived natural photosynthetic system for improving cell anabolism; Authors - Pengfei Chen
human	WB	Mater Today Bio; Platelet-derived extracellular vesicles ameliorate intervertebral disc degeneration by alleviating mitochondrial dysfunction; Authors - Zhanqiu Dai
human	WB	Redox Biol; Defective mitochondrial ISCs biogenesis switches on IRP1 to fine tune selective mitophagy.; Authors - Hao Wu
mouse	WB	EBioMedicine; DsbA-L interacts with VDAC1 in mitochondrion-mediated tubular cell apoptosis and contributes to the progression of acute kidney disease.; Authors - Xiaozhou Li
mouse	WB	Aging (Albany NY); Folic acid delays age-related cognitive decline in senescence-accelerated mouse prone 8: alleviating telomere attrition as a potential mechanism.; Authors - Xin Lv
rat	WB	Aging (Albany NY); Spermidine alleviates cardiac aging by improving mitochondrial biogenesis and function.; Authors - Junying Wang

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