NT5DC2 Polyclonal antibody

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  WB analysis using 31739-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31739-1-AP (NT5DC2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

NT5DC2 Polyclonal Antibody for WB, ELISA

Cat No. 31739-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

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Tested Applications

• Positive WB detected in: NCI-H1299 cells, SW480 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31739-1-AP targets NT5DC2 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NT5DC2 fusion protein Ag35015
• Full Name: 5'-nucleotidase domain containing 2
• Calculated Molecular Weight: 61 kDa
• Observed Molecular Weight: 60-65 kDa
• GenBank Accession Number: NM_022908
• Gene Symbol: NT5DC2
• Gene ID (NCBI): 64943
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q9H857
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

5'-Nucleotidase domain containing 2 (NT5DC2) is a member of the NT5DC family and contains a haloacid dehalogenase motif localized in the N-terminus of these proteins (PMID: 32962856). NT5DC2 is associated with attention-deficit/hyperactivity disorder and bipolar disorder. NT5DC2 has been shown to interact with and stabilize Fyn, a Src family proto-oncogene, and plays a role in regulating glioblastoma progression. NT5DC2 promotes tumor cell proliferation by stabilizing EGFR in hepatocellular carcinoma (PMID: 32382041).