NUP62 Polyclonal antibody

• Home
• Products
• Primary Antibodies
• NUP62 Polyclonal antibody (13916-1-AP)

• Featured Product
• KD/KO Validated

NUP62 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 13916-1-AP Copied!

Publications (23)

Download datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF, IP, ELISA

Synonyms

SNDI, Nucleoporin Nup62, nucleoporin 62kDa, Nuclear pore glycoprotein p62, IBSN

• 

  WB analysis of PC-3 using 13916-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 13916-1-AP (NUP62 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of NIH/3T3 using 13916-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 13916-1-AP (NUP62 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of NIH/3T3 using 13916-1-AP

  IP result of anti-NUP62 (IP:13916-1-AP, 4ug; Detection:13916-1-AP 1:1000) with NIH/3T3 cells lysate 1200ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human stomach using 13916-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13916-1-AP (NUP62 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Able AI

What's the best dilution for NUP62 in ?

Chat with Able

---

Tested Applications

• Positive WB detected in: PC-3 cells, NIH/3T3 cells
• Positive IP detected in: NIH/3T3 cells
• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13916-1-AP targets NUP62 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, monkey, chicken
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4912

  Product name: Recombinant human NUP62 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 173-522 aa of BC014842

  Sequence: IGSAGNSAQPTAPATLPFTPATPAATTAGATQPAAPTPTATITSTGPSLFASIATAPTSSATTGLSLCTPVTTAGAPTAGTQGFSLKAPGAASGTSTTTSTAATATATTTSSSSTTGFALNLKPLAPAGIPSNTAAAVTAPPGPGAAAGAAASSAMTYAQLESLINKWSLELEDQERHFLQQATQVNAWDRTLIENGEKITSLHREVEKVKLDQKRLDQELDFILSQQKELEDLLSPLEELVKEQSGTIYLQHADEEREKTYKLAENIDAQLKRMAQDLKDIIEHLNTSGAPADTSDPLQQICKILNAHMDSLQWIDQNSALLQRKVEEVTKVCEGRRKEQERSFRITFD

• Full Name: nucleoporin 62kDa
• Calculated Molecular Weight: 522 aa, 53 kDa
• Observed Molecular Weight: 62 kDa
• GenBank Accession Number: BC014842
• Gene Symbol: NUP62
• Gene ID (NCBI): 23636
• RRID: AB_2267660
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P37198
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NUP62 belongs to the nucleoporin NSP1/NUP62 family. It is essential component of the nuclear pore complex. The N-terminal is probably involved in nucleocytoplasmic transport. The C-terminal is probably involved in protein-protein interaction via coiled-coil formation and may function in anchorage of p62 to the pore complex. Defects in NUP62 are the cause of infantile striatonigral degeneration (SNDI). NUP62 consists of a single promoter with a CpG island and three transcribed exons. It is ubiquitously expressed, an observation compatible with its essential role in transporting cargo across the nuclear envelope. (PMID:16029492)

Publications

What published studies show

Anti-Nup62 (Cat# 13916-1-AP) has 23 citations published in 21 journals including Science, Nature Microbiology, Nature Physics, Molecular Cell, Journal of Clinical Investigation, Cell Reports, PLoS Pathogens, and Nature Communications. Research fields span nuclear pore complex biology, nucleocytoplasmic trafficking, phase separation, cancer, neurology, virology/immunology, lipid metabolism, autophagy, and RNA biology.

Automatically generated from the 23 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IF	Science; The mechanism of acentrosomal spindle assembly in human oocytes; Authors - Tianyu Wu
human	WB,IF	Nat Microbiol; Nuclear pore blockade reveals that HIV-1 completes reverse transcription and uncoating in the nucleus.; Authors - Adarsh Dharan
human	WB	Nat Phys; Structural anisotropy results in mechano-directional transport of proteins across nuclear pores; Authors - Fani Panagaki ; KD Validated
mouse	WB	Mol Cell; RAPIDASH: Tag-free enrichment of ribosome-associated proteins reveals composition dynamics in embryonic tissue, cancer cells, and macrophages; Authors - Teodorus Theo Susanto
mouse	WB	J Clin Invest; Sec13 promotes oligodendrocyte differentiation and myelin repair through autocrine pleiotrophin signaling.; Authors - Zhixiong Liu
		Cell Death Discov; Exon junction complex and nuclear pore complex components preferentially govern the nuclear export of spliced lncRNAs.; Authors - Sondas Saeed