NY-ESO-1 Recombinant antibody

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  WB analysis of NCI-H1299 using 85355-1-RR

  NCI-H1299 cells were subjected to SDS PAGE followed by western blot with 85355-1-RR (NY-ESO-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 85355-1-RR

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 85355-1-RR (NY-ESO-1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 85355-1-RR

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 85355-1-RR (NY-ESO-1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

NY-ESO-1 Uni-rAb^TM Recombinant Antibody for WB, IHC, ELISA

Cat No. 85355-1-RR

Clone No.242671C11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

CTAG1A, 242671C11, Autoimmunogenic cancer/testis antigen NY-ESO-1, CTAG, CTAG1

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Tested Applications

• Positive WB detected in: NCI-H1299 cells
• Positive IHC detected in: human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85355-1-RR targets NY-ESO-1 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: cancer/testis antigen 1A
• Calculated Molecular Weight: 18 kDa
• Observed Molecular Weight: 20 kDa
• GenBank Accession Number: NM_139250
• Gene Symbol: NY-ESO-1
• Gene ID (NCBI): 246100
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P78358
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CTAG1A, also named as NY-ESO-1, CTAG, CTAG1, ESO1, LAGE2, LAGE2A, CTAG1B, LAGE2B and CT6.1, belongs to the CTAG family.