Notch1 antibody (mAb) (Clone ICN 5B5)
Host / Isotype
Rat / IgG2b
Reactivity
Human, Rat
Applications
WB
CloneNo.
ICN 5B5
Cat No : 61147,61148 61147
Synonyms
Validation Data Gallery
Product Information
| Tested Applications |
WB
Applications Validated by Active Motif: WB: 1 - 2 ug/ml dilution For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all blocking solutions may also reduce background. Individual optimization may be required. This antibody is also available as an AbFlex engineered recombinant antibody. For details on the corresponding AbFlex Recombinant Antibody, see Catalog No. 91243. |
| Tested Reactivity | Human, Rat |
| Host / Isotype | Rat / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | This Notch1 antibody was raised against a recombinant protein corresponding to the intracellular domain of human Notch1. |
| Full Name | Notch1 antibody (mAb) (Clone ICN 5B5) |
| Synonyms | Notch1, Notch, transcription factor, dbdabs, Delta, Serrate, RBP-J, RBP-SUH, SUH, MAML, MAML1, ESC, stem cell, monoclonal, clone ICN 5B5, sample |
| Molecular weight | 120 kDa |
| GenBank accession number | NP_060087 |
| RRID | AB_2793526 |
| Purification Method | Protein G Chromatography |
| Buffer | Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| Storage | Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage. |
Background Information
Notch1 is a member of the Notch family of receptors, which make up a highly conserved cell signaling system. Notch1 functions as a ligand-activated transcription factor that directly transduces extracellular signals in the cell surface into changes in gene expression in the nucleus. Notch1 is membrane bound, serving as an extracellular receptor for the Delta and Serrate family of ligands. Upon activation, the intracellular domain is cleaved and rapidly translocates into the nucleus where it forms a transcriptional complex with the RBP-J DNA binding protein and activates gene expression via recruitment of co-activators of the MAML family. Notch receptors play a critical role in cell fate specification during development and participate in multiple biological processes. Notch signaling is required for undifferentiated embryonic stem cells (ESCs) to participate in the formation of all three embryonic germ layers.