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  • KD/KO Validated

OAS1 Polyclonal antibody

OAS1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 14955-1-AP

Synonyms

(2 5)oligo(A) synthase 1, 2 5 oligoadenylate synthase 1, 2 5A synthase 1, E18/E16, IFI 4, OAS1, OIAS, OIASI, p46/p42 OAS



Tested Applications

Positive WB detected inHeLa cells, mouse brain tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

14955-1-AP targets OAS1 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen OAS1 fusion protein Ag6793
Full Name 2',5'-oligoadenylate synthetase 1, 40/46kDa
Calculated molecular weight 46 kDa
Observed molecular weight 48 kDa
GenBank accession numberBC000562
Gene symbol OAS1
Gene ID (NCBI) 4938
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The 2-prime,5-prime oligoadenylate synthetases (OASs), such as OAS1, are interferon-induced proteins characterized by their capacity to catalyze the synthesis of 2-prime,5-prime oligomers of adenosine (2-5As). OAS1 (type I enzymes) have molecular weights of 42, 44, 46, and 48 kDa, form tetramers, and contain a single consensus sequence(PMID:12590567). OAS1 is a strong candidate for determining susceptibility or resistance to viral infections(PMID:15732009). This antibody may have weak cross-reaction with OAS3 protein due to the highly homology.

Protocols

Product Specific Protocols
WB protocol for OAS1 antibody 14955-1-APDownload protocol
IHC protocol for OAS1 antibody 14955-1-APDownload protocol
IF protocol for OAS1 antibody 14955-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Science

A prenylated dsRNA sensor protects against severe COVID-19.

Authors - Arthur Wickenhagen
  • KO Validated
human

Adv Sci (Weinh)

The CRISPR-Cas13a Gene-Editing System Induces Collateral Cleavage of RNA in Glioma Cells.

Authors - Qixue Wang
mouseWB

Autophagy

USP19 (ubiquitin specific peptidase 19) promotes TBK1 (TANK-binding kinase 1) degradation via chaperone-mediated autophagy.

Authors - Xibao Zhao
humanWB

J Invest Dermatol

OAS1, OAS2, and OAS3 Contribute to Epidermal Keratinocyte Proliferation by Regulating Cell Cycle and Augment Type I Interferon-Induced JAK1-STAT1 Phosphorylation in Psoriasis.

Authors - Yan-Zhou Huang
  • KD Validated
humanWB,IHC

Elife

A systematic CRISPR screen reveals an IL-20/IL20RA-mediated immune crosstalk to prevent the ovarian cancer metastasis.

Authors - Jia Li
humanWB

FASEB J

Exosomal miR-145-5p derived from orthohantavirus-infected endothelial cells inhibits HTNV infection.

Authors - Xin Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Seong (Verified Customer) (11-27-2018)

OAS1 polyclonal antibody (14955-1-AP) did work by Western Blot (1:1,500) of ARPE-19 cells induced with interferon gamma but the band was very weak in uninduced cells.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1,500
  • Cell Tissue Type: human ARPE-19 cells