OAS1 Polyclonal antibody

• Featured Product
• KD/KO Validated

OAS1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Publications

20

Reviews (1)

Cat no : 14955-1-AP

Synonyms

(2 5)oligo(A) synthase 1, 2 5 oligoadenylate synthase 1, 2 5A synthase 1, E18/E16, IFI 4, OAS1, OIAS, OIASI, p46/p42 OAS

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Validation Data Gallery

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  WB analysis of HeLa using 14955-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14955-1-AP (OAS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 14955-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14955-1-AP (OAS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 14955-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14955-1-AP (OAS1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 14955-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14955-1-AP (OAS1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human liver using 14955-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 14955-1-AP (OAS1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 14955-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 14955-1-AP (OAS1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HeLa using 14955-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 14955-1-AP (OAS1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, mouse brain tissue
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 2 publications below
• WB: See 17 publications below
• IHC: See 3 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Product Information

14955-1-AP targets OAS1 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: OAS1 fusion protein Ag6793
• Full Name: 2',5'-oligoadenylate synthetase 1, 40/46kDa
• Calculated molecular weight: 46 kDa
• Observed molecular weight: 48 kDa
• GenBank accession number: BC000562
• Gene symbol: OAS1
• Gene ID (NCBI): 4938
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The 2-prime,5-prime oligoadenylate synthetases (OASs), such as OAS1, are interferon-induced proteins characterized by their capacity to catalyze the synthesis of 2-prime,5-prime oligomers of adenosine (2-5As). OAS1 (type I enzymes) have molecular weights of 42, 44, 46, and 48 kDa, form tetramers, and contain a single consensus sequence(PMID:12590567). OAS1 is a strong candidate for determining susceptibility or resistance to viral infections(PMID:15732009). This antibody may have weak cross-reaction with OAS3 protein due to the highly homology.

Publications

Species	Application	Title
human	WB	Science; A prenylated dsRNA sensor protects against severe COVID-19.; Authors - Arthur Wickenhagen ; KO Validated
human		Adv Sci (Weinh); The CRISPR-Cas13a Gene-Editing System Induces Collateral Cleavage of RNA in Glioma Cells.; Authors - Qixue Wang
mouse	WB	Autophagy; USP19 (ubiquitin specific peptidase 19) promotes TBK1 (TANK-binding kinase 1) degradation via chaperone-mediated autophagy.; Authors - Xibao Zhao
human	WB	J Invest Dermatol; OAS1, OAS2, and OAS3 Contribute to Epidermal Keratinocyte Proliferation by Regulating Cell Cycle and Augment Type I Interferon-Induced JAK1-STAT1 Phosphorylation in Psoriasis.; Authors - Yan-Zhou Huang ; KD Validated
human	WB,IHC	Elife; A systematic CRISPR screen reveals an IL-20/IL20RA-mediated immune crosstalk to prevent the ovarian cancer metastasis.; Authors - Jia Li
human	WB	FASEB J; Exosomal miR-145-5p derived from orthohantavirus-infected endothelial cells inhibits HTNV infection.; Authors - Xin Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Seong (Verified Customer) (11-27-2018)

OAS1 polyclonal antibody (14955-1-AP) did work by Western Blot (1:1,500) of ARPE-19 cells induced with interferon gamma but the band was very weak in uninduced cells.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1,500
• Cell Tissue Type: human ARPE-19 cells