OAS1 Polyclonal Antibody for IF, IHC, WB, ELISA
Cat no : 14955-1-AP
(2 5)oligo(A) synthase 1, 2 5 oligoadenylate synthase 1, 2 5A synthase 1, E18/E16, IFI 4, OAS1, OIAS, OIASI, p46/p42 OAS
|Positive WB detected in||HeLa cells, mouse brain tissue|
|Positive IHC detected in||human liver tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:100-1:400|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
14955-1-AP targets OAS1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||OAS1 fusion protein Ag6793|
|Full Name||2',5'-oligoadenylate synthetase 1, 40/46kDa|
|Calculated molecular weight||46 kDa|
|Observed molecular weight||48 kDa|
|GenBank accession number||BC000562|
|Gene ID (NCBI)||4938|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
The 2-prime,5-prime oligoadenylate synthetases (OASs), such as OAS1, are interferon-induced proteins characterized by their capacity to catalyze the synthesis of 2-prime,5-prime oligomers of adenosine (2-5As). OAS1 (type I enzymes) have molecular weights of 42, 44, 46, and 48 kDa, form tetramers, and contain a single consensus sequence(PMID:12590567). OAS1 is a strong candidate for determining susceptibility or resistance to viral infections(PMID:15732009). This antibody may have weak cross-reaction with OAS3 protein due to the highly homology.
Myxovirus resistance protein A inhibits hepatitis C virus replication through JAK-STAT pathway activation.
Int J Mol Sci
STAT3 Regulates the Type I IFN-Mediated Antiviral Response by Interfering with the Nuclear Entry of STAT1.
Mol Cancer Res
STAT1 and NF-κB Inhibitors Diminish Basal Interferon-Stimulated Gene Expression and Improve the Productive Infection of Oncolytic HSV in MPNST Cells.
Adv Sci (Weinh)
The CRISPR-Cas13a Gene-Editing System Induces Collateral Cleavage of RNA in Glioma Cells.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Seong (Verified Customer) (11-27-2018)
OAS1 polyclonal antibody (14955-1-AP) did work by Western Blot (1:1,500) of ARPE-19 cells induced with interferon gamma but the band was very weak in uninduced cells.