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  • KD/KO Validated

OBFC2A Polyclonal antibody

OBFC2A Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 16719-1-AP

Synonyms

hSSB2, OBFC2A, Sensor of ssDNA subunit B2, SOSS B2, SOSS complex subunit B2, SSB2



Tested Applications

Positive WB detected inCOLO 320 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Sample-dependent, check data in validation data gallery

Product Information

16719-1-AP targets OBFC2A in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen OBFC2A fusion protein Ag10158
Full Name oligonucleotide/oligosaccharide-binding fold containing 2A
Calculated molecular weight 124 aa, 14 kDa
Observed molecular weight 22 kDa
GenBank accession numberBC107723
Gene symbol OBFC2A
Gene ID (NCBI) 64859
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Single-stranded DNA (ssDNA)-binding proteins (SSBs) are ubiquitous and essential for a variety of DNA metabolic processes, including recombination, replication, and detection and repair of damage. SSBs have multiple roles in binding and sequestering ssDNA, detecting DNA damage, stimulating nucleases, helicases and strand-exchange proteins, activating transcription and mediating protein-protein interactions [PMID:18449195] Human single-stranded DNA-binding protein 1 (hSSB1), encoded by OBFC2B, is characterized as an essential factor for the initiation of DNA damage checkpoints and the maintenance of genomic stability [PMID:22940690].

Protocols

Product Specific Protocols
WB protocol for OBFC2A antibody 16719-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Immunol

SSB1/SSB2 Proteins Safeguard B Cell Development by Protecting the Genomes of B Cell Precursors.

Authors - Matthias Pfeifer
  • KO Validated
mouseWB

EMBO J

The hSSB1 orthologue Obfc2b is essential for skeletogenesis but dispensable for the DNA damage response in vivo.

Authors - Feldhahn Niklas N
  • KD Validated
humanWB

Cell Res

The Integrator complex controls the termination of transcription at diverse classes of gene targets.

Authors - Jeffrey R Skaar
  • KD Validated
mouseWB

Blood

Ssb1 and Ssb2 cooperate to regulate mouse hematopoietic stem and progenitor cells by resolving replicative stress.

Authors - Wei Shi