OCT4 Antibody 35 Publications

Rabbit Polyclonal| Catalog number: 11263-1-AP

Featured Product KD/KO validated

  • Print page
  • Download PDF

Be the first to review this product


-_-

Freight/Packing

Con: 54 μg/150 μl

Choose size:

Please visit your regions distributor:


Species specificity:
human, mouse, rat

Positive WB detected in:
H7ES cells, HEK-293 cells, hESC cells, human placenta tissue, mouse brain tissue, mouse embryo tissue, mouse kidney tissue, NCCIT cell

Positive IHC detected in:
human colon cancer tissue

Positive IF detected in:
human embronic stem cells

Recommended dilution:
WB : 1:500-1:2000
IHC : 1:50-1:200
IF : 1:20-1:200

Product Information


Source:
Rabbit

Purification method:
Antigen affinity purification

Isotype:
IgG

Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
POU class 5 homeobox 1

Calculated molecular weight:
39 kDa

Observed molecular weight:
50 kDa

GenBank accession number:

Gene ID (NCBI):

Gene symbol
POU5F1

Synonyms
Oct 3, Oct 4, OCT3, OCT3/4, OCT4, Octamer binding protein 3, Octamer binding protein 4, OTF 3, OTF3, OTF4, POU class 5 homeobox 1, POU5F1
Background

Expression of the Oct-4 transcription factor is associated with an undifferentiated phenotype of human and mouse embryonic stem cells and germ cells. Oct-4 (Octamer-4) is a homeodomain transcription factor of the POU family (POU5F1) involved in regulation of pluripotency during normal development and is detectable in embryonic stem (ES) and germ cells. Its expression is associated with an undifferentiated phenotype and tumors. In fact, gene knockdown of Oct4 promotes differentiation, thereby demonstrating a role for this factor in human ES cell self-renewal. As such, it is frequently used as a marker for undifferentiated ES cells or iPS (induced pluripotent stem) cells. Oct4 is one of the four key transcription factors (Oct4, Sox2, c-Myc, and KLF4) used to reprogram mouse and human fibroblasts to a pluripotent state as well as generate patient-specific iPS cells. Affinity purified rabbit anti-Oct4 can be used to demonstrate pluripotency of ES and iPS cells. The calculated molecular weight of OCT4 is 38 kDa, but the modified OCT4 protein is about 50 kDa.


Back
to top