OIT3 Polyclonal antibody

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  WB analysis using 32301-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32301-1-AP (OIT3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

OIT3 Polyclonal Antibody for WB, ELISA

Cat No. 32301-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Liver-specific zona pellucida domain-containing protein, LZP, Oncoprotein-induced transcript 3 protein, UNQ826/PRO1753

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Tested Applications

• Positive WB detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

32301-1-AP targets OIT3 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: OIT3 fusion protein Ag36932
• Full Name: oncoprotein induced transcript 3
• Calculated Molecular Weight: 60 kDa，545aa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: NM_152635.2
• Gene Symbol: OIT3
• Gene ID (NCBI): 170392
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q8WWZ8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Oncoprotein-induced transcript 3 protein (OIT3) is a liver-specific protein. It is involved in various biological processes, including lipid metabolism and ferroptosis regulation. OIT3 has been shown to mediate macrophage polarization and facilitate hepatocellular carcinoma (HCC) progression by regulating the arachidonic acid metabolism and reactive oxygen species (ROS) accumulation (PMID: 35353239). Additionally, OIT3 is implicated in the regulation of lipid metabolism, particularly in the context of triacylglycerol and very low-density lipoproteins (VLDLs) in the liver (PMID: 36132142).