OLAH Polyclonal antibody

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  WB analysis using 17676-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17676-1-AP (OLAH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 17676-1-AP

  MCF-10A cells were subjected to SDS PAGE followed by western blot with 17676-1-AP (OLAH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

OLAH Polyclonal Antibody for WB, ELISA

Cat No. 17676-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Augmented in rheumatoid arthritis 1, AURA1, EC:3.1.2.14, Oleoyl-ACP hydrolase, S-acyl fatty acid synthase thioesterase, medium chain

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Tested Applications

• Positive WB detected in: DU 145 cells, MCF-10A cells, LNCap cells, PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17676-1-AP targets OLAH in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: OLAH fusion protein Ag11694
• Full Name: oleoyl-ACP hydrolase
• Calculated Molecular Weight: 265 aa, 30 kDa
• Observed Molecular Weight: 36 kDa
• GenBank Accession Number: BC050372
• Gene Symbol: OLAH
• Gene ID (NCBI): 55301
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NV23
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

OLAH protein, also known as oleoyl-ACP hydrolase, is an enzyme involved in fatty acid production. It has been found to play a role in various biological processes and diseases. For instance, placental OLAH levels are altered in fetal growth restriction and preeclampsia, suggesting its potential involvement in placental dysfunction. Additionally, OLAH has been linked to severe respiratory diseases, where it mediates life-threatening inflammation associated with viral infections. Its expression is also associated with fatal outcomes in certain viral infections. These findings highlight the importance of OLAH in both reproductive health and infectious diseases (PMID: 36139751, PMID: 39137778, PMID: 39178832). OLAH has two isoforms with molecular masses of 30 kDa and 36 kDa.