OLFML1 Polyclonal antibody

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  WB analysis using 31165-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31165-1-AP (OLFML1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

OLFML1 Polyclonal Antibody for WB, ELISA

Cat No. 31165-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

UNQ564, olfactomedin like 1

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Tested Applications

• Positive WB detected in: HuH-7 cells, U-251 cells, mouse testis tissue, rat testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31165-1-AP targets OLFML1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: OLFML1 fusion protein Ag35125
• Full Name: olfactomedin-like 1
• Calculated Molecular Weight: 402 aa, 46 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC092488
• Gene Symbol: OLFML1
• Gene ID (NCBI): 283298
• RRID: AB_3669879
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q6UWY5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

OLFML1 (Olfactomedin-like protein 1) is a novel member of human OLF protein family with an olfectamine domain (143-397 aa) in its C-terminus. OLFML1 exhibits the promotion of cell proliferation, along with the increase of percentage of S phase cells. OLFML1 is considered a secreted glycoprotein that enhances cell cycle progression in human cancer cell lines in vitro (PMID: 30266405). OLFML1 contains three potential N-glycosylation sites (residues 66, 138 and 183) and could be modified by the N-glycosylation (PMID: 18708057).