OPCML Polyclonal antibody

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  WB analysis using 17962-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17962-1-AP (OPCML antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

OPCML Polyclonal Antibody for WB, ELISA

Cat No. 17962-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

IgLON family member 1, IGLON1, OBCAM, OPCM, OPCML

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Tested Applications

• Positive WB detected in: mouse pancreas tissue, rat pancreas tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17962-1-AP targets OPCML in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: OPCML fusion protein Ag12560
• Full Name: opioid binding protein/cell adhesion molecule-like
• Calculated Molecular Weight: 345 aa, 38 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC074773
• Gene Symbol: OPCML
• Gene ID (NCBI): 4978
• RRID: AB_2935445
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q14982
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

OPCML, also called opioid-binding cell adhesion molecule (OBCAM), belongs to the IgLON limbic system-associated membrane protein (LSAMP, OPCML/OBCAM, neurotrimin) family of IgG domain containing glycosylphosphatidylinositol-anchored cell adhesion molecules. Originally isolated from brain, OPCMLis also expressed in stomach, ovary, heart, placenta, testis, kidney, liver, pancreas and colon. OPCML has been identified as a tumour suppressor, which inhibited tumor growth in some cancers including ovarian cancer, lung cancer and prostate cancer (PMID: 19176311; 28407749).