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  • KD/KO Validated

OS9 Polyclonal antibody

OS9 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 10061-1-AP

Synonyms

Amplified in osteosarcoma 9, OS 9, OS9, Protein OS 9



Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells
Positive IHC detected inhuman lung cancer tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10061-1-AP targets OS9 in WB, IHC, IF,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen OS9 fusion protein Ag0106
Full Name osteosarcoma amplified 9, endoplasmic reticulum associated protein
Calculated molecular weight 76 kDa
Observed molecular weight 73 kDa
GenBank accession numberBC000532
Gene symbol OS9
Gene ID (NCBI) 10956
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

OS-9 is a ubiquitously expressed ensoplasmic reticulum (ER)-associated protein originally identified as being amplified in certain osteosarcomas. It functions in ER quality control and ER-associated degradation (ERAD). There are three isoforms of this protein: OS-9.1, OS-9.2 and OS-9.3. The longest isoform, OS-9.1, contains 667 aa, OS-9.2 lacks aa 535-589, whereas OS-9.3 lacks aa 456-470 and 535-589. OS-9.1 and OS-9.2 are N-glycosylated, ubiquitously expressed in human tissues, and amplified in tumors. Isoform 2 is the major isoform detected in all cell types examined. This antibody can recognize all three isoforms of OS-9.

Protocols

Product Specific Protocols
WB protocol for OS9 antibody 10061-1-APDownload protocol
IHC protocol for OS9 antibody 10061-1-APDownload protocol
IF protocol for OS9 antibody 10061-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Biosci

Proteomic characterization of endogenous substrates of mammalian ubiquitin ligase Hrd1.

Authors - Yilin Ye
humanWB

FEBS J

Endoplasmic reticulum lectin XTP3-B inhibits endoplasmic reticulum-associated degradation of a misfolded α1-antitrypsin variant.

Authors - Fujimori Tsutomu T
  • KD Validated
humanWB

J Biol Chem

SEL1L protein critically determines the stability of the HRD1-SEL1L endoplasmic reticulum-associated degradation (ERAD) complex to optimize the degradation kinetics of ERAD substrates.

Authors - Iida Yasutaka Y
  • KD Validated
humanWB,IF

J Biol Chem

OS-9 regulates the transit and polyubiquitination of TRPV4 in the endoplasmic reticulum.

Authors - Wang Yan Y
  • KD Validated
humanWB

J Biol Chem

Human OS-9, a lectin required for glycoprotein endoplasmic reticulum-associated degradation, recognizes mannose-trimmed N-glycans.

Authors - Hosokawa Nobuko N
humanWB

J Biol Chem

Human XTP3-B forms an endoplasmic reticulum quality control scaffold with the HRD1-SEL1L ubiquitin ligase complex and BiP.

Authors - Hosokawa Nobuko N