Validation Data Gallery
|Positive WB detected in||HUVEC cells, HeLa cells, HEK-293 cells, MCF-7 cells, THP-1 cells, HepG2 cells, LNCaP cells, NCCIT cells, U2OS cells|
|Positive IP detected in||MCF-7 cells|
|Positive IHC detected in||human cervical cancer tissue, human breast cancer tissue, human colon cancer tissue, human thyroid cancer tissue, human stomach cancer tissue, human kidney tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||MCF-7 cells, myotome of a somite at E11.5 tissue|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:200-1:600|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
10355-1-AP targets P21 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human samples.
|Cited Reactivity||human, Artemia sinica, canine, pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||P21 fusion protein Ag0368|
|Full Name||cyclin-dependent kinase inhibitor 1A (p21, Cip1)|
|Calculated molecular weight||21 kDa|
|Observed molecular weight||21 kDa|
|GenBank accession number||BC000275|
|Gene ID (NCBI)||1026|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
CDKN1A (p21, CIP1, WAF1) is a cyclin-dependent kinase inhibitor. CDKN1A binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at the G1 phase. The expression of CDKN1A is induced by wild-type but not mutant p53 protein, through which CDKN1A mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. CDKN1A can interact with proliferating cell nuclear antigen (PCNA), and plays a regulatory role in S phase DNA replication and DNA damage repair. CDKN1A was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Two alternatively spliced variants, which encode an identical protein, have been reported.
|Product Specific Protocols|
|WB protocol for P21 antibody 10355-1-AP||Download protocol|
|IHC protocol for P21 antibody 10355-1-AP||Download protocol|
|IF protocol for P21 antibody 10355-1-AP||Download protocol|
|IP protocol for P21 antibody 10355-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Antrocin Sensitizes Prostate Cancer Cells to Radiotherapy through Inhibiting PI3K/AKT and MAPK Signaling Pathways.
Ubiquinol-cytochrome C reductase core protein II promotes tumorigenesis by facilitating p53 degradation.
Aging (Albany NY)
lncRNA-ES3/miR-34c-5p/BMF axis is involved in regulating high-glucose-induced calcification/senescence of VSMCs.
J Cell Physiol
Histone demethylase KDM6B regulates 1,25-dihydroxyvitamin D3-induced senescence in glioma cells.
Toxicol In Vitro
SIRT1 suppresses p53-dependent apoptosis by modulation of p21 in osteoblast-like MC3T3-E1 cells exposed to fluoride.
p53/Lactate dehydrogenase A axis negatively regulates aerobic glycolysis and tumor progression in breast cancer expressing wild-type p53.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Malak (Verified Customer) (04-22-2021)
The best incubation period for this antibody is 1.5 hr at room temperature, longer incubations (especially overnight at 4°C) may result in aspecific bands.
Susan (Verified Customer) (12-03-2019)
We had been having issues with our old P21 antibody and this one worked great.
Béatrice (Verified Customer) (09-04-2019)
This is an excellent and specific antibody, without background.When prepared in 3%BSA, it can be reused several times and kept at-20°C