P21;CDKN1A Antibody 76 Publications

Rabbit Polyclonal| Catalog number: 10355-1-AP

Featured Product KD/KO validated

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Freight/Packing

Con: 58 μg/150 μl

Choose size:

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Species specificity:
human

Positive WB detected in:
MCF-7 cells,HUVEC cells, HEK-293 cells, HEK-293T cells, HeLa cells

Positive IP detected in:
MCF-7 cells

Positive IHC detected in:
human cervical cancer tissue,human breast cancer tissue, human colon cancer tissue, human thyroid cancer tissue, human stomach cancer tissue, human kidney tissue

Positive IF detected in:
MCF-7 cells,myotome of a somite at E11.5 tissue

Positive FC detected in:
HeLa cells

Recommended dilution:
WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
IHC : 1:50-1:500
IF : 1:50-1:500

Product Information


Source:
Rabbit

Purification method:
Antigen affinity purification

Isotype:
IgG

Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
cyclin-dependent kinase inhibitor 1A (p21, Cip1)

Calculated molecular weight:
21 kDa

Observed molecular weight:
21 kDa

GenBank accession number:

Gene ID (NCBI):

Gene symbol
CDKN1A

Synonyms
CAP20, CDK interacting protein 1, CDKN1, CDKN1A, CIP1, MDA 6, MDA6, P21, p21CIP1, PIC1, SDI1, WAF1
Background

CDKN1A (p21, CIP1, WAF1) is a cyclin-dependent kinase inhibitor. CDKN1A binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at the G1 phase. The expression of CDKN1A is induced by wild-type but not mutant p53 protein, through which CDKN1A mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. CDKN1A can interact with proliferating cell nuclear antigen (PCNA), and plays a regulatory role in S phase DNA replication and DNA damage repair. CDKN1A was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Two alternatively spliced variants, which encode an identical protein, have been reported.


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