P21 Polyclonal antibody

• Featured Product
• KD/KO Validated

P21 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (3)

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Publications

582

Reviews (4)

Cat no : 10355-1-AP

Synonyms

CAP20, CDK interacting protein 1, CDKN1, CDKN1A, CIP1, MDA 6, MDA6, P21, p21CIP1, PIC1, SDI1, WAF1

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Validation Data Gallery

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  WB analysis using 10355-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10355-1-AP (P21 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10355-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10355-1-AP (P21 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 10355-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 10355-1-AP (P21 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10355-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10355-1-AP (P21 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10355-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10355-1-AP (P21 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10355-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10355-1-AP (P21 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of MCF-7 using 10355-1-AP

  IP result of anti-P21;CDKN1A (IP:10355-1-AP, 4ug; Detection:10355-1-AP 1:1000) with MCF-7 cells lysate 2800 ug.

• 

  IHC staining of human cervical cancer using 10355-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10355-1-AP (P21;CDKN1A Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 10355-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10355-1-AP (P21;CDKN1A Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10355-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10355-1-AP (P21;CDKN1A antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10355-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10355-1-AP (P21;CDKN1A antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 10355-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 10355-1-AP (P21;CDKN1A antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 10355-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 10355-1-AP (P21;CDKN1A antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 10355-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10355-1-AP (P21;CDKN1A antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 10355-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10355-1-AP (P21;CDKN1A antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 10355-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 10355-1-AP (P21 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 10355-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 10355-1-AP (P21 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 10355-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10355-1-AP (P21;CDKN1A antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10355-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10355-1-AP (P21;CDKN1A antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 10355-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 10355-1-AP (P21;CDKN1A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of myotome of a somite at E11.5 tissue using 10355-1-AP

  IF result of anti-p21 (CDKN1A, 10355-1-AP) in the myotome of a somite at E11.5 by Dr. Zalc A. and Dr. Relaix F. (Red = p21; Green=Pax3 from a GFP reporter; Blue=DAPI).

• 

  IF Staining of MCF-7 using 10355-1-AP

  Immunofluorescent analysis of MCF-7 cells using 10355-1-AP (P21;CDKN1A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of MCF-7 using 10355-1-AP

  1X10^6 MCF-7 cells were intracellularly stained with 0.2 ug Anti-Human P21 (10355-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive WB detected in: HUVEC cells, MCF-7 cells, HeLa cells, HEK-293 cells, HepG2 cells, THP-1 cells, LNCaP cells, NCCIT cells, U2OS cells
• Positive IP detected in: MCF-7 cells
• Positive IHC detected in: human cervical cancer tissue, human breast cancer tissue, human colon cancer tissue, human thyroid cancer tissue, human stomach cancer tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: MCF-7 cells, myotome of a somite at E11.5 tissue
• Positive FC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
• Immunohistochemistry (IHC): IHC : 1:200-1:600
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 9 publications below
• WB: See 542 publications below
• IHC: See 44 publications below
• IF: See 27 publications below
• IP: See 1 publications below
• FC: See 1 publications below
• CoIP: See 2 publications below

Product Information

10355-1-AP targets P21 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, Artemia sinica, canine, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: P21 fusion protein Ag0368
• Full Name: cyclin-dependent kinase inhibitor 1A (p21, Cip1)
• Calculated molecular weight: 21 kDa
• Observed molecular weight: 21 kDa
• GenBank accession number: BC000275
• Gene symbol: CDKN1A
• Gene ID (NCBI): 1026
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CDKN1A (p21, CIP1, WAF1) is a cyclin-dependent kinase inhibitor. CDKN1A binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at the G1 phase. The expression of CDKN1A is induced by wild-type but not mutant p53 protein, through which CDKN1A mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. CDKN1A can interact with proliferating cell nuclear antigen (PCNA), and plays a regulatory role in S phase DNA replication and DNA damage repair. CDKN1A was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Two alternatively spliced variants, which encode an identical protein, have been reported.

Publications

Species	Application	Title
human	WB,IHC	Gastroenterology; Intestinal PPARα Protects Against Colon Carcinogenesis via Regulation of Methyltransferases DNMT1 and PRMT6.; Authors - Yuhong Luo
human,mouse	WB	Nat Commun; Phosphoglycerate dehydrogenase activates PKM2 to phosphorylate histone H3T11 and attenuate cellular senescence; Authors - Yinsheng Wu
human	WB	Mol Cancer; CircVAPA promotes small cell lung cancer progression by modulating the miR-377-3p and miR-494-3p/IGF1R/AKT axis.; Authors - Jinghan Hua
human	WB	Mol Cancer; LncRNA TROJAN promotes proliferation and resistance to CDK4/6 inhibitor via CDK2 transcriptional activation in ER+ breast cancer.; Authors - Xi Jin
human	WB,IHC	Mol Cancer; Circular RNA hsa_circ_0004872 inhibits gastric cancer progression via the miR-224/Smad4/ADAR1 successive regulatory circuit.; Authors - Cunying Ma
mouse	WB	Dev Cell; Stem cell competition driven by the Axin2-p53 axis controls brain size during murine development; Authors - Xue-Lian Sun

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Hadil (Verified Customer) (05-24-2023)

I prepared this antibody in odyssey blocker at 1:1000 and incubated at 4 C overnight and seemed to get a good signal for p21. There were some non-specific bands properly caused by long incubation periods. So, i would recommend using it at this concentration for 1.5-2 hr at RT.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mammalian cell lines

FH

Malak (Verified Customer) (04-22-2021)

The best incubation period for this antibody is 1.5 hr at room temperature, longer incubations (especially overnight at 4°C) may result in aspecific bands.

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: Hepatic cancer cell extract

FH

Susan (Verified Customer) (12-03-2019)

We had been having issues with our old P21 antibody and this one worked great.

• Applications: Western Blot,
• Cell Tissue Type: keratinocytes, dog

FH

Béatrice (Verified Customer) (09-04-2019)

This is an excellent and specific antibody, without background.When prepared in 3%BSA, it can be reused several times and kept at-20°C

• Applications: Western Blot,
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HCT116

P21 Antibody Western Blot, validation (1/1000 dilution) in HCT116 (Cat no:10355-1-AP)

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