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  • KD/KO Validated

P21 Monoclonal antibody

P21 Monoclonal Antibody for ELISA

Host / Isotype

Mouse / IgG2b


monkey and More (1)







Cat no : 60214-1-Ig


CAP20, CDK interacting protein 1, CDKN1, CDKN1A, CIP1, MDA 6, MDA6, P21, p21CIP1, PIC1, SDI1, WAF1

Tested Applications

Recommended dilution

Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 60214-1-Ig is P21 Fusion Protein expressed in E. coli.

Tested Reactivity monkey
Cited Reactivityhuman
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen P21 fusion protein Ag0308
Full Name cyclin-dependent kinase inhibitor 1A (p21, Cip1)
Calculated molecular weight 21 kDa
GenBank accession numberBC000312
Gene symbol CDKN1A
Gene ID (NCBI) 1026
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CDKN1A (p21, CIP1, WAF1), a cyclin-dependent kinase inhibitor, is necessary for proper control of the cell cycle and premature senescence. P21 is a cyclin-dependent kinase (CDK) inhibitor that suppresses proliferation by inhibiting CDK2 and CDK1 activity at the G1/S and G2/M transitions. P21 is a major mediator of p53 to induce cell cycle arrest in G1. p21 can interact with proliferating cell nuclear antigen (PCNA), and plays a regulatory role in S phase DNA replication and DNA damage repair. P21 was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Overexpression of p21 decreases the expression levels of cell cycle progression genes and upregulates senescence-inducing genes.


Product Specific Protocols
WB protocol for P21 antibody 60214-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



J Exp Clin Cancer Res

GTSE1 is involved in breast cancer progression in p53 mutation-dependent manner.

Authors - Fen Lin

J Cell Physiol

HOXC13-AS promotes breast cancer cell growth through regulating miR-497-5p/PTEN axis.

Authors - Xiaowei Li

Bosn J Basic Med Sci

CircRNA_014511 affects the radiosensitivity of bone marrow mesenchymal stem cells by binding to miR-29b-2-5p.

Authors - Yanjie Wang

Ann Clin Lab Sci

Yeast Extract Induces Apoptosis and Cell Cycle Arrest via Activating p38 Signal Pathway in Colorectal Cancer Cells.

Authors - Daeun Moon

Cell Death Dis

N-glycosylation of GDF15 abolishes its inhibitory effect on EGFR in AR inhibitor-resistant prostate cancer cells

Authors - Rong Wang

Open Med (Wars)

S100A6 promotes proliferation and migration of HepG2 cells via increased ubiquitin-dependent degradation of p53.

Authors - Dongqiang Song


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Ying (Verified Customer) (02-02-2021)

it is very good antibody.

  • Applications: Western Blot

Chunxia (Verified Customer) (02-02-2021)

Tissue culture

  • Applications: Western Blot, Cell culture