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- KD/KO Validated
P21 Monoclonal antibody
P21 Monoclonal Antibody for WB, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
human
Applications
WB,ELISA
Conjugate
Unconjugated
CloneNo.
5F2F3
Cat no : 60214-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | MCF-7 cells, HEK-293 cells, HeLa cells, HUVEC cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 13 publications below |
Product Information
The immunogen of 60214-1-Ig is P21 Fusion Protein expressed in E. coli.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | P21 fusion protein Ag0308 |
| Full Name | cyclin-dependent kinase inhibitor 1A (p21, Cip1) |
| Calculated molecular weight | 21 kDa |
| Observed molecular weight | 21 kDa |
| GenBank accession number | BC000312 |
| Gene symbol | CDKN1A |
| Gene ID (NCBI) | 1026 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CDKN1A (p21, CIP1, WAF1), a cyclin-dependent kinase inhibitor, is necessary for proper control of the cell cycle and premature senescence. P21 is a cyclin-dependent kinase (CDK) inhibitor that suppresses proliferation by inhibiting CDK2 and CDK1 activity at the G1/S and G2/M transitions. P21 is a major mediator of p53 to induce cell cycle arrest in G1. p21 can interact with proliferating cell nuclear antigen (PCNA), and plays a regulatory role in S phase DNA replication and DNA damage repair. P21 was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Overexpression of p21 decreases the expression levels of cell cycle progression genes and upregulates senescence-inducing genes.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for P21 antibody 60214-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Exp Clin Cancer Res GTSE1 is involved in breast cancer progression in p53 mutation-dependent manner. | ||
J Cell Physiol HOXC13-AS promotes breast cancer cell growth through regulating miR-497-5p/PTEN axis. | ||
Bosn J Basic Med Sci CircRNA_014511 affects the radiosensitivity of bone marrow mesenchymal stem cells by binding to miR-29b-2-5p. | ||
Oncol Res Treat GAS5 Inhibits Gastric Cancer Cell Proliferation Partly by Modulating CDK6. | ||
Biomed Pharmacother MiRNA-20a-5p promotes the growth of triple-negative breast cancer cells through targeting RUNX3. | ||
Food Nutr Res Cyanidin-3-O-glucoside inhibits epithelial-to-mesenchymal transition, and migration and invasion of breast cancer cells by upregulating KLF4. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Ying (Verified Customer) (02-02-2021) | it is very good antibody.
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FH Chunxia (Verified Customer) (02-02-2021) | Tissue culture
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