P21 Monoclonal antibody

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  WB analysis of MCF-7 using 60214-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 60214-1-Ig (P21 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 60214-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 60214-1-Ig (P21 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• Featured Product
• KD/KO Validated

P21 Monoclonal Antibody for ELISA

Cat No. 60214-1-Ig

Clone No.5F2F3

Publications(31)

Reviews (2)

Host / Isotype

Mouse / IgG2b

Reactivity

monkey and More (1)

Applications

WB, ELISA

Synonyms

CAP20, CDK interacting protein 1, CDKN1, CDKN1A, CIP1, MDA 6, MDA6, P21, p21CIP1, PIC1, SDI1, WAF1

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Published Applications

• KD/KO: See 1 publications below
• WB: See 29 publications below

Product Information

60214-1-Ig targets P21 in WB, ELISA applications and shows reactivity with monkey samples.

• Tested Reactivity: monkey
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: P21 fusion protein Ag0308
• Full Name: cyclin-dependent kinase inhibitor 1A (p21, Cip1)
• Calculated Molecular Weight: 21 kDa
• GenBank Accession Number: BC000312
• Gene Symbol: p21
• Gene ID (NCBI): 1026
• RRID: AB_11042450
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P38936
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CDKN1A (p21, CIP1, WAF1), a cyclin-dependent kinase inhibitor, is necessary for proper control of the cell cycle and premature senescence. P21 is a cyclin-dependent kinase (CDK) inhibitor that suppresses proliferation by inhibiting CDK2 and CDK1 activity at the G1/S and G2/M transitions. P21 is a major mediator of p53 to induce cell cycle arrest in G1. p21 can interact with proliferating cell nuclear antigen (PCNA), and plays a regulatory role in S phase DNA replication and DNA damage repair. P21 was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Overexpression of p21 decreases the expression levels of cell cycle progression genes and upregulates senescence-inducing genes.

Publications

Species	Application	Title
human	WB	Adv Sci (Weinh); Identification of PRDX5 as A Target for The Treatment of Castration-Resistant Prostate Cancer; Authors - Rong Wang
human	WB	Biomed Pharmacother; MiRNA-20a-5p promotes the growth of triple-negative breast cancer cells through targeting RUNX3.; Authors - Xiangdong Bai
human	WB	J Pathol; NFIB promotes cell survival by directly suppressing p21 transcription in p53-mutated triple-negative breast cancer.; Authors - Rong-Zong Liu ; KD Validated
human	WB	Virulence; Inhibition of cell proliferation by Tas of foamy viruses through cell cycle arrest or apoptosis underlines the different mechanisms of virus-host interactions.; Authors - Wei Jie
		Int J Biol Sci; Discovery of a novel HDACi structure that inhibits the proliferation of ovarian cancer cells in vivo and in vitro.; Authors - Miao Bai
	WB	Aging (Albany NY); Transgenic mice overexpressing glia maturation factor-β, an oxidative stress inducible gene, show premature aging due to Zmpste24 down-regulation.; Authors - Rika Imai

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Ying (Verified Customer) (02-02-2021)

it is very good antibody.

• Applications: Western Blot

FH

Chunxia (Verified Customer) (02-02-2021)

Tissue culture

• Applications: Western Blot, Cell culture