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  • KD/KO Validated

P27; KIP1 Polyclonal antibody

P27; KIP1 Polyclonal Antibody for WB, IP, IF, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse and More (4)





Cat no : 25614-1-AP


CDKN1B, CDKN4, KIP1, MEN1B, MEN4, P27, P27; KIP1, P27KIP1

Tested Applications

Positive WB detected inHeLa cells, NIH/3T3 cells, HepG2 cells, MCF-7 cells, Jurkat cells
Positive IP detected inNIH/3T3 cells
Positive IHC detected inhuman breast cancer tissue, human lung cancer tissue, human colon cancer tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, mouse testis tissue
Positive FC detected inMCF-7 cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25614-1-AP targets P27; KIP1 in WB, IP, IF, FC, IHC, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat, sheep, canine, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen P27; KIP1 fusion protein Ag22582
Full Name cyclin-dependent kinase inhibitor 1B (p27, Kip1)
Calculated Molecular Weight 198 aa, 22 kDa
Observed Molecular Weight 27 kDa
GenBank Accession NumberBC001971
Gene Symbol CDKN1B
Gene ID (NCBI) 1027
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CDKN1B, also named as P27 or KIP1, is a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. P27 binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controlling cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Downregulation of P27 has been implicated in the progression of several malignancies, including lung cancer, hepatocellular carcinoma, salivary cancer, oral squamous cell carcinomas, and gastric cancer.


Product Specific Protocols
WB protocol for P27; KIP1 antibody 25614-1-APDownload protocol
IHC protocol for P27; KIP1 antibody 25614-1-APDownload protocol
IF protocol for P27; KIP1 antibody 25614-1-APDownload protocol
IP protocol for P27; KIP1 antibody 25614-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Malak (Verified Customer) (04-22-2021)

The optimal incubation period is 1.5 hr at room temperature. Avoid incubating the antibody overnight as it may result in aspecific signals.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/2000
  • Cell Tissue Type: Hepatic cancer cell extract

Susmita (Verified Customer) (09-17-2020)

Antibody is working very good for WB

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000 WB;

Joshua (Verified Customer) (07-27-2019)

Cells fixed in 4% paraformaldehyde and stained at 4C overnight. Decent staining with some background in rabbit cells. Nuclear localization as expected.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Rabbit Corneal Epithelial Cells
P27; KIP1 Antibody Immunofluorescence, validation (1:500 dilution) in Rabbit Corneal Epithelial Cells (Cat no:25614-1-AP)