P2RX7 Polyclonal antibody

P2RX7 Polyclonal Antibody for FC, IF, IHC,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, FC,ELISA

Conjugate

Unconjugated

Cat no : 11144-1-AP

Synonyms

P2RX7, P2X7



Tested Applications

Positive IHC detected inhuman prostate cancer tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

11144-1-AP targets P2RX7 in WB, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen P2RX7 fusion protein Ag1600
Full Name purinergic receptor P2X, ligand-gated ion channel, 7
Calculated molecular weight 69 kDa
GenBank accession numberBC011913
Gene symbol P2RX7
Gene ID (NCBI) 5027
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Nucleotides, the structural subunits of the nucleic acids, are also important extracellular signaling molecules. P2 receptors are a family of cell surface receptors that mediate a wide variety of physiologic effects in response to extracellular nucleotides. These receptors fall into two classes: P2X receptors, which are ligand-gated ion channels that mediate calcium and potassium fluxes in response to ATP, and P2Y receptors, which are G protein-coupled receptors (GPCRs) (PMID: 21724990). P2RX7 functions as a ligand-gated ion channel and is responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules. P2RX7 is highly expressed by cells of the haemopoietic lineage and can mediate cell death, killing of infectious organisms, and regulation of the inflammatory response (PMID: 22102807).

Protocols

Product Specific Protocols
IHC protocol for P2RX7 antibody 11144-1-APDownload protocol
IF protocol for P2RX7 antibody 11144-1-APDownload protocol
FC protocol for P2RX7 antibody 11144-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Oncol Rep

Emodin attenuated severe acute pancreatitis via the P2X ligand‑gated ion channel 7/NOD‑like receptor protein 3 signaling pathway.

Authors - Qingkai Zhang
mouseWB

Biol Pharm Bull

Ketamine Alleviates Depressive-Like Behaviors via Down-Regulating Inflammatory Cytokines Induced by Chronic Restraint Stress in Mice.

Authors - Sijie Tan
mouseWB,IHC

Biochem Biophys Res Commun

Muscle death participates in myofibrillar abnormalities in FHL1 knockout mice.

Authors - Jingjing Ding
mouseWB

Life Sci

Trichostatin A inhibits skeletal muscle atrophy induced by cigarette smoke exposure in mice.

Authors - Jingjing Ding
humanIF

Stem Cell Res

The P2X7 receptor is an upstream regulator of dynamic blebbing and a pluripotency marker in human embryonic stem cells.

Authors - Nikki Jo-Hao Weng
mouseWB

Am J Physiol Cell Physiol

Regulatory function of praja ring finger ubiquitin ligase 2 mediated by the P2rx3/P2rx7 axis in mouse hippocampal neuronal cells.

Authors - Mengting Gong