P2RX7 Polyclonal antibody

P2RX7 Polyclonal Antibody for IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 11144-1-AP


P2RX7, P2X7

Tested Applications

Positive IHC detected inhuman prostate cancer tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11144-1-AP targets P2RX7 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen P2RX7 fusion protein Ag1600
Full Name purinergic receptor P2X, ligand-gated ion channel, 7
Calculated Molecular Weight 69 kDa
GenBank Accession NumberBC011913
Gene Symbol P2RX7
Gene ID (NCBI) 5027
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Nucleotides, the structural subunits of the nucleic acids, are also important extracellular signaling molecules. P2 receptors are a family of cell surface receptors that mediate a wide variety of physiologic effects in response to extracellular nucleotides. These receptors fall into two classes: P2X receptors, which are ligand-gated ion channels that mediate calcium and potassium fluxes in response to ATP, and P2Y receptors, which are G protein-coupled receptors (GPCRs) (PMID: 21724990). P2RX7 functions as a ligand-gated ion channel and is responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules. P2RX7 is highly expressed by cells of the haemopoietic lineage and can mediate cell death, killing of infectious organisms, and regulation of the inflammatory response (PMID: 22102807).


Product Specific Protocols
IHC protocol for P2RX7 antibody 11144-1-APDownload protocol
IF protocol for P2RX7 antibody 11144-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



J Immunol

The danger signal, extracellular ATP, is a sensor for an airborne allergen and triggers IL-33 release and innate Th2-type responses.

Authors - Kouzaki Hideaki H

Front Cell Neurosci

Clemastine Alleviates Depressive-Like Behavior Through Reversing the Imbalance of Microglia-Related Pro-inflammatory State in Mouse Hippocampus.

Authors - Wen-Jun Su

J Leukoc Biol

HMG-CoA reductase inhibitors activate caspase-1 in human monocytes depending on ATP release and P2X7 activation.

Authors - Liao Yi-Hsiang YH

J Exp Clin Cancer Res

Shock wave-induced ATP release from osteosarcoma U2OS cells promotes cellular uptake and cytotoxicity of methotrexate.

Authors - Baochang Qi

J Biochem Mol Toxicol

Blockade of P2X7 receptor-mediated purinergic signaling with A438079 protects against LPS-induced liver injury in rats

Authors - Adem Kara

J Biochem Mol Toxicol

Assessment of toxicological effects of favipiravir (T-705) on the lung tissue of rats: An experimental study

Authors - Elif Erbaş