P2RX7 Polyclonal antibody

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  WB analysis using 11144-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11144-1-AP (P2RX7 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 11144-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11144-1-AP (P2RX7 Antibody) at dilution of 1:400 (under 40x lens).

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  FC experiment of RAW 264.7 using 11144-1-AP

  1x10^6 RAW 264.7 cells were intracellularly stained with 0.5 ug P2RX7 Polyclonal antibody (11144-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.5 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

P2RX7 Polyclonal Antibody for WB, FC (Intra), ELISA

Cat No. 11144-1-AP

Publications(21)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, FC (Intra), ELISA

Synonyms

P2X7, Purinergic receptor

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Tested Applications

• Positive WB detected in: mouse brain tissue, mouse cerebellum tissue
• Positive FC (Intra) detected in: RAW 264.7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 17 publications below
• IHC: See 3 publications below
• IF: See 4 publications below

Product Information

11144-1-AP targets P2RX7 in WB, IHC, IF, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: P2RX7 fusion protein Ag1600
• Full Name: purinergic receptor P2X, ligand-gated ion channel, 7
• Calculated Molecular Weight: 69 kDa
• Observed Molecular Weight: 70-80 kDa
• GenBank Accession Number: BC011913
• Gene Symbol: P2RX7
• Gene ID (NCBI): 5027
• RRID: AB_2158371
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q99572
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Nucleotides, the structural subunits of the nucleic acids, are also important extracellular signaling molecules. P2 receptors are a family of cell surface receptors that mediate a wide variety of physiologic effects in response to extracellular nucleotides. These receptors fall into two classes: P2X receptors, which are ligand-gated ion channels that mediate calcium and potassium fluxes in response to ATP, and P2Y receptors, which are G protein-coupled receptors (GPCRs) (PMID: 21724990). P2RX7 functions as a ligand-gated ion channel and is responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules. P2RX7 is highly expressed by cells of the haemopoietic lineage and can mediate cell death, killing of infectious organisms, and regulation of the inflammatory response (PMID: 22102807).

Publications

Species	Application	Title
human	IF	J Immunol; The danger signal, extracellular ATP, is a sensor for an airborne allergen and triggers IL-33 release and innate Th2-type responses.; Authors - Kouzaki Hideaki H
mouse	WB	Front Cell Neurosci; Clemastine Alleviates Depressive-Like Behavior Through Reversing the Imbalance of Microglia-Related Pro-inflammatory State in Mouse Hippocampus.; Authors - Wen-Jun Su
		J Leukoc Biol; HMG-CoA reductase inhibitors activate caspase-1 in human monocytes depending on ATP release and P2X7 activation.; Authors - Liao Yi-Hsiang YH
human	IF	J Exp Clin Cancer Res; Shock wave-induced ATP release from osteosarcoma U2OS cells promotes cellular uptake and cytotoxicity of methotrexate.; Authors - Baochang Qi
rat	WB,IHC	J Biochem Mol Toxicol; Blockade of P2X7 receptor-mediated purinergic signaling with A438079 protects against LPS-induced liver injury in rats; Authors - Adem Kara
rat	WB	J Biochem Mol Toxicol; Assessment of toxicological effects of favipiravir (T-705) on the lung tissue of rats: An experimental study; Authors - Elif Erbaş