P2RY2 Polyclonal antibody

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  WB analysis using 20297-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20297-1-AP (P2RY2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

P2RY2 Polyclonal Antibody for WB, ELISA

Cat No. 20297-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

P2U receptor 1, P2U purinoceptor 1, P2U, P2RU1, HP2U

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Tested Applications

• Positive WB detected in: HL-60 cells, MCF-7 cells, PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

20297-1-AP targets P2RY2 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: P2RY2 fusion protein Ag14107
• Full Name: purinergic receptor P2Y, G-protein coupled, 2
• Calculated Molecular Weight: 377 aa, 42 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC012104
• Gene Symbol: P2RY2
• Gene ID (NCBI): 5029
• RRID: AB_3669332
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P41231
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

P2RY2, also named P2RU1, P2U1, and P2Y2, belongs to the G-protein coupled receptor 1 family. It is a receptor for ATP and UTP coupled to G-proteins that activate a phosphatidylinositol-calcium second messenger system. Extracellular ATP then acts on purinergic receptors, including P2RY2, to activate proline-rich tyrosine kinase 2 (Pyk2) kinase and transient plasma membrane depolarization.