• Featured Product
  • KD/KO Validated

P53 Polyclonal antibody

P53 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse and More (5)

Applications

WB, IP, IF, CoIP, chIP, ELISA

Conjugate

Unconjugated

Cat no : 10442-1-AP

Synonyms

Antigen NY CO 13, Cellular tumor antigen p53, LFS1, P53, Phosphoprotein p53, TP53, TRP53, tumor protein p53, Tumor suppressor p53



Tested Applications

Positive WB detected inSMMC-7721 cells, HEK-293 cells, MCF-7 cells, rat colon tissue, mouse spleen tissue, A431 cells, mouse colon tissue
Positive IP detected inA431 cells
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10442-1-AP targets P53 in WB, IP, IF, CoIP, chIP, ELISA applications and shows reactivity with human, rat, mouse samples.

Tested Reactivity human, rat, mouse
Cited Reactivity Artemia, Artemia sinica, human, pig, rat, sheep, swine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen P53 fusion protein Ag0698
Full Name tumor protein p53
Calculated molecular weight 44 kDa
Observed molecular weight 53 kDa
GenBank accession numberBC003596
Gene symbol TP53
Gene ID (NCBI) 7157
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

1.       What is p53?

P53 is a tumor suppressor gene that plays a role in maintaining genomic stability and controlling apoptosis. During the cell cycle, it can arrest cells at the G1/S checkpoint and activate DNA repair mechanisms. It is the most mutated gene in cancer. In unstressed cells, p53 usually exists at low levels in an inactive form, being bound to Mdm2.

2.       FAQs and p53

a.       I fail to detect p53 by western blotting

Basal levels of wild-type p53 in untreated cells can be low. Try to load more cell lysate and use a positive control – a lysate of cells treated with DNA-damaging agents should increase p53 levels.

b.      I fail to detect p53 in some cell lines by western blotting

Various p53 mutations are present in cancer cell types. If mutations cause truncations/deletions some monoclonal antibodies may no longer recognize mutated p53. You have more chances of detecting various p53 mutants with our polyclonal antibody.

c.       I can detect more than one band ~50 kDa size / different cell lines give bands at slightly different size

p53 is a subject of post-translational modifications (http://p53.free.fr/p53_info/p53_modifications.html) and more than one isoform may be expressed (http://p53.free.fr/p53_info/p53_isoforms.html). Also, it is possible that your cell line of interest expresses one allele with mutated p53 with altered molecular weight.


Protocols

Product Specific Protocols
WB protocol for P53 antibody 10442-1-APDownload protocol
IF protocol for P53 antibody 10442-1-APDownload protocol
IP protocol for P53 antibody 10442-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Toxicol Appl Pharmacol

PMID: 30597158

Authors - Jichen Sha
humanWB

Cancer Sci

PMID: 30417588

Authors - Chao Qu
humanWB,IF

Int J Biochem Cell Biol

Sinoporphyrin sodium based sonodynamic therapy induces anti-tumor effects in hepatocellular carcinoma and activates p53/caspase 3 axis.

Authors - Enze Li
WB

Biochem Cell Biol

Knockdown of RNF2 enhances radiosensitivity of lung squamous cell carcinoma cells.

Authors - Jie Yang
humanWB,IP

EBioMedicine

Ubiquinol-cytochrome C reductase core protein II promotes tumorigenesis by facilitating p53 degradation.

Authors - Yingyan Han
humanWB

Cancer Lett

Anterior Gradient-2 monoclonal antibody inhibits lung cancer growth and metastasis by upregulating p53 pathway and without exerting any toxicological effects: A preclinical study.

Authors - Hema Negi

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

WEI (Verified Customer) (01-17-2020)

Weak band in heart lysates

  • Applications: Western Blot,
  • Cell Tissue Type: Mouse Heart
FH

Ben (Verified Customer) (09-26-2019)

It worked very well for our westerns.

  • Applications: Western Blot,
FH

Sid (Verified Customer) (04-12-2019)

Product performed well in western for human samples, moderate background/non specific binding

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: Human embryonic kidney
FH

Josiah (Verified Customer) (02-27-2019)

Antibody performed superb in a Western Blot application. Used Licor IR secondary antibodies to label the p53 antibody along with ß-actin as a duplex assay and imaged on a Licor Odyssey FC.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: T8
P53 Antibody Western Blot, validation (1:1000 dilution) in T8 (Cat no:10442-1-AP)