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P53 Monoclonal antibody
P53 Monoclonal Antibody for ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugate
Unconjugated
CloneNo.
2D2F10
Cat no : 60283-1-Ig
Synonyms
Validation Data Gallery
Published Applications
KD/KO | See 2 publications below |
WB | See 23 publications below |
IHC | See 1 publications below |
IF | See 1 publications below |
IP | See 1 publications below |
Product Information
60283-1-Ig targets P53 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | P53 fusion protein Ag0698 |
Full Name | tumor protein p53 |
Calculated Molecular Weight | 44 kDa |
GenBank Accession Number | BC003596 |
Gene Symbol | P53 |
Gene ID (NCBI) | 7157 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TP53, also named as P53 and NY-CO-13, belongs to the p53 family. It has 9 isoforms. In SDS-Page, the MW is about 53kd. TP53 acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. It is involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. The antibody reacts specifically with 53 kDa protein.
Protocols
Product Specific Protocols | |
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WB protocol for P53 antibody 60283-1-Ig | Download protocol |
IHC protocol for P53 antibody 60283-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Mol Ther Nucleic Acids miR-552 Regulates Liver Tumor-Initiating Cell Expansion and Sorafenib Resistance. | ||
Oncogenesis MeCP2 facilitates breast cancer growth via promoting ubiquitination-mediated P53 degradation by inhibiting RPL5/RPL11 transcription. | ||
Cancer Lett TAZ regulates cell proliferation and sensitivity to vitamin D3 in intrahepatic cholangiocarcinoma.
| ||
Am J Cancer Res Asparaginyl endopeptidase (AEP) regulates myocardial apoptosis in response to radiation exposure via alterations in NRF2 activation. | ||
Am J Cancer Res Loss of BRMS2 induces cell growth inhibition and translation capacity reduction in colorectal cancer cells. | ||
Biochem Pharmacol Ketoplatin in triple-negative breast cancer cells MDA-MB-231: High efficacy and low toxicity, and positive impact on inflammatory microenvironment. |