- Featured Product
- KD/KO Validated
P53 Monoclonal antibody
P53 Monoclonal Antibody for ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
CloneNo.
2D2F10
Cat no : 60283-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Recommended dilution
| Application | Dilution |
|---|---|
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 20 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
| IP | See 1 publications below |
Product Information
60283-1-Ig targets P53 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | P53 fusion protein Ag0698 |
| Full Name | tumor protein p53 |
| Calculated molecular weight | 44 kDa |
| GenBank accession number | BC003596 |
| Gene symbol | TP53 |
| Gene ID (NCBI) | 7157 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TP53, also named as P53 and NY-CO-13, belongs to the p53 family. It has 9 isoforms. In SDS-Page, the MW is about 53kd. TP53 acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. It is involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. The antibody reacts specifically with 53 kDa protein.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for P53 antibody 60283-1-Ig | Download protocol |
| IHC protocol for P53 antibody 60283-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Exp Clin Cancer Res IPO5 promotes the proliferation and tumourigenicity of colorectal cancer cells by mediating RASAL2 nuclear transportation. | ||
Biomed Res Int Uncovering the Pharmacology of Xiaochaihu Decoction in the Treatment of Acute Pancreatitis Based on the Network Pharmacology. | ||
Curr Med Sci BOP1 Silencing Suppresses Gastric Cancer Proliferation through p53 Modulation. | ||
Oncol Lett 14-3-3ε is a nuclear matrix protein, and its altered expression and localization are associated with curcumin-induced apoptosis of MG-63 cells. | ||
Can J Gastroenterol Hepatol TRIM29 Reverses Oxaliplatin Resistance of P53 Mutant Colon Cancer Cell. |
