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P53 Monoclonal antibody

P53 Monoclonal Antibody for ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2D2F10

Cat no : 60283-1-Ig

Synonyms

Antigen NY CO 13, Cellular tumor antigen p53, LFS1, Phosphoprotein p53, TRP53, tumor protein p53, Tumor suppressor p53



Tested Applications

Recommended dilution

ApplicationDilution
Sample-dependent, check data in validation data gallery

Product Information

60283-1-Ig targets P53 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen P53 fusion protein Ag0698
Full Name tumor protein p53
Calculated molecular weight 44 kDa
GenBank accession numberBC003596
Gene symbol TP53
Gene ID (NCBI) 7157
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TP53, also named as P53 and NY-CO-13, belongs to the p53 family. It has 9 isoforms. In SDS-Page, the MW is about 53kd. TP53 acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. It is involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. The antibody reacts specifically with 53 kDa protein.

Protocols

Product Specific Protocols
WB protocol for P53 antibody 60283-1-IgDownload protocol
IHC protocol for P53 antibody 60283-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Front Endocrinol (Lausanne)

PMID: 31024445

Authors - Wei Ding
humanWB

J Exp Clin Cancer Res

IPO5 promotes the proliferation and tumourigenicity of colorectal cancer cells by mediating RASAL2 nuclear transportation.

Authors - Wenjuan Zhang
ratWB

Biomed Res Int

Uncovering the Pharmacology of Xiaochaihu Decoction in the Treatment of Acute Pancreatitis Based on the Network Pharmacology.

Authors - Lianghui Zhan
humanWB

Curr Med Sci

BOP1 Silencing Suppresses Gastric Cancer Proliferation through p53 Modulation.

Authors - Yu-Peng Yang
humanIF

Oncol Lett

14-3-3ε is a nuclear matrix protein, and its altered expression and localization are associated with curcumin-induced apoptosis of MG-63 cells.

Authors - Kun Lu
humanWB

Can J Gastroenterol Hepatol

TRIM29 Reverses Oxaliplatin Resistance of P53 Mutant Colon Cancer Cell.

Authors - Guoqiong Lei