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P53 Monoclonal antibody
P53 Monoclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
Human, rat, mouse and More (4)
Applications
WB, IP, IHC, IF, CoIP, ELISA
Conjugate
Unconjugated
124
CloneNo.
6C4B6
Cat no : 60283-2-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | Neuro-2a cells, A431 cells, A375 cells, COLO 320 cells, HSC-T6 cells, ROS1728 cells, Sp2/0 cells, HaCaT cells, HT-29 cells, HEK-293 cells |
| Positive IHC detected in | human colon cancer tissue, human lung cancer tissue, human breast cancer tissue, human stomach cancer tissue, human tonsillitis tissue, human gliomas tissue, human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:20000 |
| Immunohistochemistry (IHC) | IHC : 1:1600-1:6400 |
| Immunofluorescence (IF) | IF : 1:200-1:800 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 4 publications below |
| WB | See 108 publications below |
| IHC | See 15 publications below |
| IF | See 14 publications below |
| IP | See 4 publications below |
| CoIP | See 2 publications below |
Product Information
60283-2-Ig targets P53 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with Human, rat, mouse samples.
| Tested Reactivity | Human, rat, mouse |
| Cited Reactivity | human, mouse, rat, canine, pig, rabbit |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | P53 fusion protein Ag0698 |
| Full Name | tumor protein p53 |
| Calculated molecular weight | 44 kDa |
| Observed molecular weight | 53 kDa |
| GenBank accession number | BC003596 |
| Gene symbol | TP53 |
| Gene ID (NCBI) | 7157 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
1. What is p53?
P53 is a tumor suppressor gene that plays a role in maintaining genomic stability and controlling apoptosis. During the cell cycle, it can arrest cells at the G1/S checkpoint and activate DNA repair mechanisms. It is the most mutated gene in cancer. In unstressed cells, p53 usually exists at low levels in an inactive form, being bound to Mdm2.
2. FAQs and p53
a. I fail to detect p53 by western blotting
Basal levels of wild-type p53 in untreated cells can be low. Try to load more cell lysate and use a positive control - a lysate of cells treated with DNA-damaging agents should increase p53 levels.
b. I fail to detect p53 in some cell lines by western blotting
Various p53 mutations are present in cancer cell types. If mutations cause truncations/deletions some monoclonal antibodies may no longer recognize mutated p53. You have more chances of detecting various p53 mutants with our polyclonal antibody.
c. I can detect more than one band ~50 kDa size / different cell lines give bands at slightly different size
p53 is a subject of post-translational modifications (http://p53.free.fr/p53_info/p53_modifications.html) and more than one isoform may be expressed (http://p53.free.fr/p53_info/p53_isoforms.html). Also, it is possible that your cell line of interest expresses one allele with mutated p53 with altered molecular weight.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for P53 antibody 60283-2-Ig | Download protocol |
| IHC protocol for P53 antibody 60283-2-Ig | Download protocol |
| IF protocol for P53 antibody 60283-2-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Redox Biol Deficiency of S100 calcium binding protein A9 attenuates vascular dysfunction in aged mice | ||
Redox Biol SSBP1 drives high fructose-induced glomerular podocyte ferroptosis via activating DNA-PK/p53 pathway. | ||
J Cachexia Sarcopenia Muscle MyD88 deficiency ameliorates weight loss caused by intestinal oxidative injury in an autophagy-dependent mechanism. | ||
Oncogene The deubiquitinase USP7 regulates oxidative stress through stabilization of HO-1. | ||
Cell Mol Gastroenterol Hepatol Mannan-binding lectin via interaction with cell surface calreticulin promotes senescence of activated hepatic stellate cells to limit liver fibrosis progression. | ||
Int J Mol Sci Scd1 Deficiency in Early Embryos Affects Blastocyst ICM Formation through RPs-Mdm2-p53 Pathway |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Chiara (Verified Customer) (03-10-2023) | The antibody work well and was specific on the target
 |
FH Janelle (Verified Customer) (09-12-2022) | Specific to target
|
FH Allison (Verified Customer) (08-19-2019) | The antibody specifications online were easy to follow. I used the recommended dilution of antibody of 1:2000 which worked and could probably be diluted further.
|
