|Positive WB detected in||HCT 116 cells, mouse testis tissue, rat testis tissue, PC-3 cells, HeLa cells|
|Positive IP detected in||mouse testis tissue|
|Positive IHC detected in||human pancreas tissue, human testis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||MCF-7 cells|
|Positive FC detected in||MCF-7 cells|
|Western Blot (WB)||WB : 1:1000-1:5000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
10970-1-AP targets PABPC1,PABP in WB, IP, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||PABPC1,PABP fusion protein Ag1422|
|Full Name||poly(A) binding protein, cytoplasmic 1|
|Calculated molecular weight||71 kDa|
|Observed molecular weight||71 kDa|
|GenBank accession number||BC015958|
|Gene ID (NCBI)||26986|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
The poly(A)-binding protein (PABP), which is found complexed to the 3-prime poly(A) tail of eukaryotic mRNA, is required for poly(A) shortening and translation initiation [PMID: 21989405]. Polyadenylate-binding protein 1 (PABPC1) is a cytoplasmic-nuclear shuttling protein important for protein translation initiation, and both RNA processing and stability. In the cytoplasm, PABPC1 binds to the 3' poly(A) tail of eukaryotic mRNAs through its RNA-recognition motifs (RRM) and interacts with the N-terminus of eIF4G, part of the eIF4F complex associated with the 5' cap structure [PMID:20009508, 17381337].
|Product Specific Protocols|
|WB protocol for PABPC1,PABP antibody 10970-1-AP||Download protocol|
|IHC protocol for PABPC1,PABP antibody 10970-1-AP||Download protocol|
|IF protocol for PABPC1,PABP antibody 10970-1-AP||Download protocol|
|IP protocol for PABPC1,PABP antibody 10970-1-AP||Download protocol|
|FC protocol for PABPC1,PABP antibody 10970-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Purification of cross-linked RNA-protein complexes by phenol-toluol extraction.
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Zee (Verified Customer) (01-28-2020)
It worked very well when I performed western blot.
George (Verified Customer) (09-11-2019)
Rb PABP Ig shows very little endogenous background staining with clear dispersed staining seen both in the nucleus and cytoplasm. 90mins heat shock at 42oC led to granular formation in the cytoplasm