PABPC1,PABP Polyclonal antibody

• Featured Product
• KD/KO Validated

PABPC1,PABP Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA

Conjugate

Unconjugated

Publications

35

Reviews (3)

Cat no : 10970-1-AP

Synonyms

PABP, PABP1, PABPC1, PABPC 1, PABP-1

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Validation Data Gallery

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  WB analysis using 10970-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10970-1-AP (PABPC1,PABP antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10970-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10970-1-AP (PABPC1,PABP antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10970-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10970-1-AP (PABPC1,PABP antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10970-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10970-1-AP (PABPC1,PABP antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10970-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10970-1-AP (PABPC1,PABP antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat testis using 10970-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 10970-1-AP (PABPC1,PABP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 10970-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 10970-1-AP (PABPC1,PABP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 10970-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 10970-1-AP (PABPC1,PABP antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 10970-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 10970-1-AP (PABPC1,PABP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10970-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10970-1-AP (PABPC1,PABP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse testis using 10970-1-AP

  IP result of anti-PABPC1,PABP (IP:10970-1-AP, 4ug; Detection:10970-1-AP 1:700) with mouse testis tissue lysate 4000ug.

• 

  IHC staining of human pancreas using 10970-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 10970-1-AP (PABPC1,PABP antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human pancreas using 10970-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 10970-1-AP (PABPC1,PABP antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 10970-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10970-1-AP (PABPC1,PABP Antibody) at dilution of 1:50.

• 

  IHC staining of human testis using 10970-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10970-1-AP (PABPC1,PABP Antibody) at dilution of 1:50.

• 

  IF Staining of MCF-7 using 10970-1-AP

  Immunofluorescent analysis of MCF-7 cells, using PABPC1 antibody 10970-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: HCT 116 cells, mouse testis tissue, PC-3 cells, HeLa cells, A549 cells, rat testis tissue, HT-29 cells, MCF-7 cells, NCI-H1299 cells, HCT-116 cells
• Positive IP detected in: mouse testis tissue
• Positive IHC detected in: human pancreas tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 25 publications below
• IHC: See 3 publications below
• IF: See 8 publications below
• IP: See 5 publications below
• CoIP: See 1 publications below
• RIP: See 2 publications below

Product Information

10970-1-AP targets PABPC1,PABP in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PABPC1,PABP fusion protein Ag1422
• Full Name: poly(A) binding protein, cytoplasmic 1
• Calculated Molecular Weight: 71 kDa
• Observed Molecular Weight: 71 kDa
• GenBank Accession Number: BC015958
• Gene Symbol: PABPC1
• Gene ID (NCBI): 26986
• RRID: AB_10596918
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The poly(A)-binding protein (PABP), which is found complexed to the 3-prime poly(A) tail of eukaryotic mRNA, is required for poly(A) shortening and translation initiation [PMID: 21989405]. Polyadenylate-binding protein 1 (PABPC1) is a cytoplasmic-nuclear shuttling protein important for protein translation initiation, and both RNA processing and stability. In the cytoplasm, PABPC1 binds to the 3' poly(A) tail of eukaryotic mRNAs through its RNA-recognition motifs (RRM) and interacts with the N-terminus of eIF4G, part of the eIF4F complex associated with the 5' cap structure [PMID:20009508, 17381337].

Publications

Species	Application	Title
mouse	IF	Brain Behav Immun; Transcriptomic and proteomic profiling of bi-partite and tri-partite murine iPSC-derived neurospheroids under steady-state and inflammatory condition; Authors - Julia Di Stefano
human	WB	Exp Mol Med; The deubiquitinating enzyme STAMBP is a newly discovered driver of triple-negative breast cancer progression that maintains RAI14 protein stability; Authors - Qianqian Yang
human	WB	Nat Commun; An oncopeptide regulates m6A recognition by the m6A reader IGF2BP1 and tumorigenesis.; Authors - Song Zhu
mouse	WB	Dev Cell; DDX20 is required for cell-cycle reentry of prospermatogonia and establishment of spermatogonial stem cell pool during testicular development in mice; Authors - Dingfeng Zou
human	WB	J Exp Med; E3 ligase MKRN3 is a tumor suppressor regulating PABPC1 ubiquitination in non-small cell lung cancer.; Authors - Ke Li
human	WB	Clin Transl Med; LncRNA BC promotes lung adenocarcinoma progression by modulating IMPAD1 alternative splicing; Authors - Qi Wen Chen

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Elisa (Verified Customer) (01-13-2023)

PABPC1 (Poly(A) Binding Protein Cytoplasmic 1) staining (in magenta) and Hoechst (nuclear staining) in blue. PABPC1 ab shows a clear citoplasma staining. Method: RPE1 cells were fixed in cold methanol for 10' at -20C. Cells were then rehydrated with PBS for 5'. Membrane permeabilization was then performed with 0.1% Triton + 0.1% Tween +0.01%SDS in PBS for 10'. Cells were finally incubated with blocking buffer (5% BSA+ 0.1% Tween in PBS) for 30' at RT. Primary antibody was diluted in blocking buffer 1:200 and incubated for 1h at room temperature. Alexa-488-Anti-rabbit was used as secondary antibody (1:600 dilution) (1h at room temperature).

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Human retinal pigment epithelial-1 (RPE-1)

PABPC1,PABP Antibody Immunofluorescence validation (1:200 dilution) in Human retinal pigment epithelial-1 (RPE-1) (Cat no:10970-1-AP)

FH

Zee (Verified Customer) (01-28-2020)

It worked very well when I performed western blot.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse heart tissue

FH

George (Verified Customer) (09-11-2019)

Rb PABP Ig shows very little endogenous background staining with clear dispersed staining seen both in the nucleus and cytoplasm. 90mins heat shock at 42oC led to granular formation in the cytoplasm

• Applications: Immunohistochemistry, Other,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Spinal Cord/Cortical Slice Culture